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Composition, applications thereof and moisture retention preparation

A technology of composition and enzymatic hydrolysis product, which is applied in the direction of drug combination, cosmetic preparations, skin care preparations, etc., and can solve the problems of unsatisfactory moisturizing effect and single product cost

Inactive Publication Date: 2015-12-23
伍曾利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently reported research on polypeptide moisturizing is to extract polypeptide products from crab, shellfish, seaweed or fish for moisturizing. The cost of the product is single, and the moisturizing effect is not ideal.

Method used

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  • Composition, applications thereof and moisture retention preparation
  • Composition, applications thereof and moisture retention preparation
  • Composition, applications thereof and moisture retention preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] The preparation of embodiment 1 shiitake mushroom extract

[0094] ①. Crush the fruiting bodies of dried shiitake mushrooms, add 10 times the weight of water, stir evenly, heat to 95°C, maintain for 90min, and cool down to room temperature;

[0095] ②, step ① Ultrasonic treatment of the aqueous solution, adjust the pH value to 8.5, add protease with a specific activity of 50,000 international units per kg of dried shiitake mushrooms, and hydrolyze at 50°C for 180 minutes;

[0096] ③. Adjustment step ②The pH value of the hydrolyzate is 5.0, add protease (alkaline protease, trypsin or papain) with a specific activity of 50,000 international units per kg of dried shiitake mushrooms, hydrolyze at 50°C for 360min; inactivate the enzyme, and cool it down to room temperature ;

[0097] ④. Adjust the pH value of the inactivated hydrolyzate to 7.0 in step ③, heat to 95°C, keep for 180min, cool down to room temperature, centrifugally filter, and collect the filtrate;

[0098] 5...

Embodiment 2

[0099] The preparation of embodiment 2 cucumber extract

[0100] ①. Take fresh cucumbers, squeeze the juice with a juicer, collect the cucumber juice, add 5 times the amount of deionized water to the cucumber dregs, stir evenly, heat to 80°C, keep for 1 hour, cool down to room temperature, and add 30,000 deionized water per kg of cucumber dregs. The plant compound hydrolase with international unit specific activity is used for enzymolysis, the enzymolysis temperature is 50°C, and the enzymolysis time is 1 hour. After the enzymolysis is completed, the enzyme is inactivated, filtered, and the filtrate is taken for use.

[0101] ②. Combine cucumber juice and filtrate, add 5 / 10,000 carrageenan by liquid weight, stir thoroughly, place at 4°C for 2 hours, centrifuge, and take clear liquid, which is cucumber extract.

Embodiment 3

[0102] The preparation of embodiment 3 collagen polypeptides

[0103] ①. Take the fish skin and scales as the leftovers of aquatic product processing, wash them with tap water, add purified water 8 times the weight of the fish skin and scales, and ultrafinely pulverize them. After pulverization, the fish skin and scales can pass through a 200-mesh sieve, and set aside;

[0104] ②. Adjust the pH value to 8.5 with 10.0 mol / L lye, maintain the pH value at 8.5, add alkaline protease (add 0.1% alkaline protease by weight of fish skin and scale) for enzymolysis for 120 minutes, and the enzymatic hydrolysis temperature is 55°C. After enzymatic hydrolysis, heat up to 90°C-100°C for 10 minutes to inactivate the enzyme, then cool down to 60°C for later use;

[0105] ③. Add 0.5% activated carbon for decolorization, maintain the temperature at 60°C for 60 minutes, stir for decolorization, filter, adjust the pH value to 6.0 with 10.0mol / L citric acid solution, and obtain collagen polypepti...

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Abstract

The invention relates to the biological technology field, and especially relates to a composition, applications thereof and a moisture retention preparation. The composition comprises mushrooms or mushroom extract products, cucumbers or cucumber extract products, collagen Mushroom polypeptides are employed as a characteristic raw material, natural cucumber extracting solution, collagen polypeptides and aloe extracting solution are added for blending, subpackaging is carried out and products are obtained. The provided product is suitable for people of all ages, and has obvious functions of moisture retention, whitening and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composition and its use, and a moisturizing preparation. Background technique [0002] Shiitake mushroom is the second largest edible fungus in the world, and it is known as "mountain delicacy" among Chinese people. According to analysis, the edible portion of dried shiitake mushrooms accounts for 72%, and every 100g edible portion contains 13g of protein. Shiitake mushrooms contain active ingredients such as lentinan and lentinan, which have high medicinal value. Many experts and scholars at home and abroad pay great attention to the research of lentinan. In order to pursue high-purity lentinan, lentinan peptides are often used in the preparation of lentinan Discard waste. [0003] With the rise of people's quality of life, people pay more and more attention to the research and application of pure natural moisturizing products. Lentinus edodes polypeptide is a polypeptide extra...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K8/97A61K8/64A23L1/29A61Q19/02A61Q19/00A61P17/00
Inventor 伍曾利
Owner 伍曾利
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