Biological probe used for detecting tumour marker galectin-1 and preparation method thereof

A technology for galectin and tumor marker, which is applied in the field of biological probes for detecting tumor marker galectin-1 and its preparation, can solve the problems of low absolute value and the like, and achieves strong binding ability, high efficiency and specificity The effect of sex detection

Inactive Publication Date: 2015-12-16
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the blood galectin-1 concentration of cancer patients is higher than that of normal people, its absolute value is still very low (generally at the ng / ml level), so biological probes with very high sensitivity and specificity are required for detection

Method used

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  • Biological probe used for detecting tumour marker galectin-1 and preparation method thereof
  • Biological probe used for detecting tumour marker galectin-1 and preparation method thereof
  • Biological probe used for detecting tumour marker galectin-1 and preparation method thereof

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preparation example Construction

[0048] The second aspect of the present invention provides a method for preparing a biological probe for detecting the tumor marker galectin-1, comprising the following steps:

[0049] (1) Provide gold nanorods;

[0050] (2) Lactose is provided, and lactose is produced sulfhydryl-containing lactose after sulfhydrylation reaction;

[0051] (3) Mix gold nanorods and thiol-containing lactose, and let stand at room temperature for 8-15 hours to obtain a bioprobe for detecting the tumor marker galectin-1. The bioprobe is thiol-containing lactose passed through gold - Complexes formed by sulfur bonds connected to gold nanorods.

[0052] The thiol-containing lactose is

[0053] The molar ratio of thiol-containing lactose to gold nanorods is 1×10 4 :1-3×10 4 :1.

[0054] thiol-containing lactose The preparation method is:

[0055] (a) the structural formula is The compound 1 and the structure are Compound 2 was stirred and reacted at room temperature for 36-72 hours unde...

Embodiment 1

[0087] A method for preparing a biological probe for detecting tumor marker galectin-1, comprising the following steps:

[0088] (1) Preparation of gold nanorods by seed induction method:

[0089] 0.5mM HAuCl 4 Dissolve in 0.2M CTAB solution, stir vigorously, add appropriate amount of 10mM cold NaHB 4 solution, stirred at 37°C for 2 hours to prepare a gold nanoseed solution. Then in 5mM HAuCl 4 Add 0.2MCTAB, 0.1MAgNO to the solution 3 , 1.2M HCl and 10mM ascorbic acid (AA), when the solution turns from dark red to colorless, add the gold nano seed solution prepared above, incubate at 37°C for 12 hours and then centrifuge, remove the supernatant and rinse the residue with ultrapure water Redispersion was carried out to obtain an aqueous solution of gold nanorods (CTAB-GNRs) whose surface was covered by CTAB.

[0090] (2) Preparation of thiol-containing lactose using compound 1 as a raw material, comprising the following steps:

[0091] Wherein the preparation method of co...

Embodiment 2

[0107] A method for preparing a biological probe for detecting tumor marker galectin-1, comprising the following steps:

[0108] (1) Preparation of gold nanorods by seed induction method:

[0109] 0.5mM HAuCl 4 Dissolve in 0.2M CTAB solution, stir vigorously, add appropriate amount of 10mM cold NaHB 4 solution, stirred at 37°C for 2 hours to prepare a gold nanoseed solution. Then in 5mM HAuCl 4 Add 0.2MCTAB, 0.1MAgNO to the solution 3 , 1.2M HCl and 10mM ascorbic acid, when the solution turns from dark red to colorless, add the gold nano seed solution prepared above, incubate at 37°C for 12 hours and then centrifuge, remove the supernatant and redisperse the residue with ultrapure water , to obtain an aqueous solution of gold nanorods (CTAB-GNRs) whose surface is covered by CTAB.

[0110] (2) Preparation of thiol-containing lactose using compound 1 as a raw material, comprising the following steps:

[0111] Wherein the preparation method of compound 1 is:

[0112] Add s...

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Abstract

The invention provides a biological probe used for detecting tumour marker galectin-1, and the biological probe is a compound formed by connecting sulfhydryl-containing lactose with a gold nanorod via a gold-sulfur bond. The sulfhydryl-containing lactose is firstly used for modifying the gold nanorod, the sulfhydryl-containing lactose is connected with the gold nanorod via the gold-sulfur bond, and the combination capability is strong. The gold nanorod is loaded with the carbohydrate micromolecule lactose and is subjected to stable specific modification, the lactose part in the obtained probe possesses specific recognition effect on galectin-1, and additionally because of the precise optical response effect of the gold nanorod, finally the biological probe is capable of efficiently specifically detecting galectin-1. Furthermore, the invention also provides a preparation method for the biological probe used for detecting the tumour marker galectin-1, and the preparation method for the biological probe is simple and practicable, mild in conditions and relatively low in cost.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a biological probe for detecting tumor marker galectin-1 and a preparation method thereof. Background technique [0002] Cancer is a disease that seriously threatens human life and health around the world. An important reason why advanced cancer patients are difficult to cure is that cancer is prone to metastasis. The primary tumor can be removed surgically or treated with radiation, but cancer that has spread is often difficult to treat with these methods and can easily damage normal tissue during treatment. Tumor metastasis is accompanied by poor prognosis and low survival rate. If an effective detection method can be developed for early monitoring, it will help timely detection and early intervention of metastasis, which will help prolong the life of patients and improve their quality of life. . [0003] Detection of tumor markers in blood can help realize early diagnos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484
Inventor 赵岳涛喻学峰潘浩波王怀雨
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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