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Application of a thermophilic chaetomium cellulase in pulp and papermaking process

A thermophilic Chaetomium and cellulase technology, which is applied in textiles and papermaking, fiber raw material treatment, enzymes, etc., can solve the problems of acid cellulase inactivation, low interlayer bonding strength, and poor fiber bonding force. Achieve the effects of reducing the content of fine fibers, no obvious changes in bulk and stiffness, and improving the bond strength and tensile strength between the paper layers

Active Publication Date: 2017-07-04
SHANDONG YOUTELL BIOCHEM +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is to provide a new application of Chaetomium thermophila cellulase, that is, the application in the pulping and papermaking process, which solves the following problems in the prior art: under neutral conditions, acid fiber Sulfase is seriously inactivated, and pH fluctuations have a greater impact on enzyme activity, resulting in poor fiber bonding, resulting in problems such as low paper strength, low interlayer bonding strength, and poor printability.

Method used

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  • Application of a thermophilic chaetomium cellulase in pulp and papermaking process
  • Application of a thermophilic chaetomium cellulase in pulp and papermaking process
  • Application of a thermophilic chaetomium cellulase in pulp and papermaking process

Examples

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Effect test

Embodiment 1

[0036] Example 1 Preparation of Chaetomium thermophila cellulase expressed by Trichoderma reesei (see Example 1 of the specification of the invention patent application publication CN103343111A with the patent application number 201310280267.6).

Embodiment 2

[0037] Example 2 Preparation of Chaetomium thermophila cellulase expressed by Pichia pastoris (see Example 3 of the specification of the invention patent application publication CN103343111A with the patent application number 201310280267.6).

Embodiment 3

[0038] Example 3 Qualitative test of Chaetomium thermophila cellulase as neutral cellulase in papermaking process (enzyme activity survival rate is above 90%, and enzyme activity is less affected by pH in the neutral range)

[0039] The specific steps and methods of cellulase CMC enzyme activity test are as follows:

[0040] 1. Principle

[0041] Cellulase hydrolyzes cellulose to produce monosaccharides and oligosaccharides under certain temperature and pH conditions. Oligosaccharides with reducing ends and monosaccharides with reducing groups undergo a color reaction with DNS reagent under boiling water bath conditions. The intensity of the color of the reaction solution is proportional to the amount of reducing sugar produced by enzymatic hydrolysis. The amount of reducing sugar produced is related to the reaction. The cellulase activity in the solution is proportional to the absorbance of the reaction solution by spectrocolorimetry, and the cellulase activity can be calculat...

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Abstract

The invention discloses application of chaetomium thermophilum cellulase in the pulping and papermaking process. The chaetomium thermophilum cellulase is expressed through trichoderma reesei or pichia pastoris. The chaetomium thermophilum cellulase is neutral cellulase. In the pulping and papermaking process, the optimal effect pH range of the chaetomium thermophilum cellulase is 6.0 to 7.0, and use cost is reduced. The influences of normal fluctuation of the pH of a neutral papermaking system on the chaetomium thermophilum cellulase are low. Frame needle pulp and frame width pulp are pre-treated before pulping of the chaetomium thermophilum cellulase, frame needle pulp and frame width pulp fibers can be modified, and therefore the binding force between the fibers is improved; the interlayer bonding strength of finished paper and the tensile strength are remarkably improved, and the bulkness and stiffness are not obviously changed.

Description

technical field [0001] The invention relates to the field of biological enzyme preparations, in particular to a new application of a chaetomyces thermophila cellulase, in particular to an application of a chaetomyces thermophila cellulase in pulp and papermaking processes. Background technique [0002] With the development of biological science and technology, biotechnology has been more and more widely used in the pulp and paper industry, and has become the focus of research. Biotechnology applied to raw material pulping can reduce energy consumption, reduce pulp hardness, and improve pulp performance; biotechnology applied to pulp bleaching can improve pulp bleachability, reduce the amount of chemical bleaching auxiliaries, and reduce the pollution load of bleaching wastewater. The application of biotechnology in the pulp and paper industry has largely solved a series of technical, pollution, energy consumption and other problems in the traditional pulp and paper process, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42D21C5/00
Inventor 慈元钊李新良张民
Owner SHANDONG YOUTELL BIOCHEM
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