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Application of d-borneol serving as antitumor drug sensitizer

An anti-tumor drug, dextroborneol technology, applied in the direction of anti-tumor drugs, drug combinations, medical preparations containing active ingredients, etc., to achieve the effect of low cytotoxicity

Inactive Publication Date: 2015-12-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about D-borneol having the function of antitumor drug sensitizer

Method used

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  • Application of d-borneol serving as antitumor drug sensitizer
  • Application of d-borneol serving as antitumor drug sensitizer
  • Application of d-borneol serving as antitumor drug sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 D-borneol increases the sensitivity of non-drug-resistant tumor cells to chemical drugs

[0051] (1) Experimental materials

[0052] The malignant melanoma cells A375 used in this experiment were purchased from the American Type Culture Collection (ATCC, Manassas, VA); D-borneol was purchased from the National Institute for the Control of Pharmaceutical and Biological Products; Curcumin was purchased from Sigma Company; Methylazolium blue (MTT) powder and dimethyl sulfoxide (DMSO) were purchased from Sigma Corporation, USA; DMEM culture medium and trypsin were obtained from Invitrogen (Carlsbad, CA) Corporation, USA; 96-well plates were purchased from Corning Corporation, USA.

[0053] (2) Experimental method

[0054] A375 cells were cultured in DMEM medium containing 10% fetal bovine serum by volume. After the cells were cultured for 3 days, the growth status and cell density of the cells were observed. When the cells grew to 80%, the cells were subcultured. ...

Embodiment 2

[0059] Example 2 D-borneol increases the sensitivity of non-drug-resistant tumor cells to chemical drugs

[0060] (1) Experimental materials

[0061] The breast cancer cells MCF-7 used in this experiment were purchased from the American Type Culture Collection (ATCC, Manassas, VA); D-borneol was purchased from the National Institute for the Control of Pharmaceutical and Biological Products; curcumin was purchased from Sigma; Tetramethylazolium blue (MTT) powder and dimethyl sulfoxide (DMSO) were purchased from Sigma Corporation, USA; DMEM culture medium and trypsin were obtained from Invitrogen (Carlsbad, CA) Corporation, USA; 96-well plates were purchased from Corning Corporation, USA.

[0062] (2) Experimental method

[0063] Operation is with embodiment 1.

[0064] (3) Experimental results

[0065] The concentration of dextroborneol used in this experiment has no obvious growth inhibition on MCF-7. Through preliminary experiments, the concentration of d-borneol was dete...

Embodiment 340

[0068] Example 340 μg / ml NB+20 μM Cur acts on A375 cells

[0069] 1. Detection of curcumin content in A375 cells

[0070] (1) Experimental materials

[0071] NaOH solution containing 1% (v / v) TritionX-100 (the concentration of NaOH is 0.1mol / L), referred to as cell lysate.

[0072] (2) Experimental method

[0073] A375 cells in the logarithmic growth phase were taken, digested with trypsin, and counted to 8×10 4 Cells / well were seeded in a 96-well culture plate and placed in an incubator (37°C, 5% CO 2 ) after culturing for 24h. Add 40 μg / ml NB, 20 μM Cur and 40 μg / ml NB+20 μM Cur (this group was treated with NB for 12 h before adding Cur) for 0.5 h, 1 h, 2 h and 4 h, remove the culture medium, and add 1 mL of LPBS (0.01 M, pH 7 .2, the same below) Wash the cells 3 times to remove extracellular borneol and curcumin, then add 200 μL of cell lysate, vibrate for 10 min, and measure the content of curcumin with a fluorescent microplate reader. Wherein, the excitation wavelen...

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Abstract

The invention discloses an application of d-borneol serving as an antitumor drug sensitizer. According to the application of d-borneol serving as the antitumor drug sensitizer, d-borneol serving as the antitumor drug sensitizer is used for assisting in antitumor drugs. The inventor discovers that after the concentration of d-borneol which has no remarkable growth influence on cells is increased, the sensitivity of non-resistant cells to antitumor chemical drugs is greatly improved, that is, the concentration of the antitumor chemical drugs for killing tumor cells can be reduced on the basis of addition of d-borneol, the curative effect is not reduced, toxic and side effects of the chemical drugs are reduced along with reduction of the concentration of the chemical drugs, and normal cells of people are protected. D-borneol has low toxicity for normal cells of people and does not have remarkable influence basically, and accordingly, d-borneol is a potential high-efficient low-toxicity chemical drug sensitizer.

Description

technical field [0001] The invention relates to the use of d-borneol, in particular to the application of d-borneol as an antitumor drug sensitizer. Background technique [0002] At present, the treatment of cancer is mainly based on surgical resection. Since cancer cells are prone to metastasis, surgery cannot cure the disease. Radiotherapy and chemotherapy are often supplemented. Although radiotherapy has a faster effect than chemotherapy, it also brings a lot of side effects to patients. Therefore, chemical drug therapy (chemotherapy) is sent to be the main means to alleviate the pain of patients. [0003] Over the past half century, tumor chemotherapy has developed rapidly. Various antineoplastic drugs such as doxorubicin, daunorubicin, cisplatin, 5-fluorouracil, paclitaxel, etc. have been applied in clinic one after another, bringing good news to patients. However, chemotherapy will inevitably produce many toxic and side effects, such as antineoplastic drugs such as a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/045A61K45/06A61P35/00
CPCA61K31/045A61K45/06
Inventor 苏健裕陈建平李琳
Owner SOUTH CHINA UNIV OF TECH
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