Method for making transparent parusvenustulus skeleton specimen

A technology for making transparent bones and specimens, which is applied in the preservation, application, and animal husbandry of human or animal bodies. Production efficiency, good degreasing effect

Inactive Publication Date: 2015-12-16
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used transparent agents for transparent treatment are potassium hydroxide or sodium hydroxide. Potassium hydroxide and sodium hydroxide have good transparent effects, but they are highly corrosive. When the concentration exceeds 3.5% or the transparent time is too long, the bones of the specimen are likely to be brittle and cracked. due color and integrity, resulting in production failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for preparing a transparent skeleton specimen of a yellow-bellied titmouse, comprising the following steps:

[0020] Step 1, take the dead yellow-bellied tit and fix it with 4% formaldehyde solution for 10 days;

[0021] Step 2, using 50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethanol from low concentration to high concentration to carry out gradient dehydration to the yellow-bellied tit after the fixed treatment of step one, 7 days for each concentration gradient;

[0022] Step 3, degreasing the dehydrated yellow-bellied chickadee in a sodium hydroxide solution with a mass concentration of 1% for 3-4 days, and then degreasing with xylene for 1 day;

[0023] Step 4, put the yellow-bellied tit degreased in step 3 into a potassium hydroxide solution with a mass concentration of 1% for transparent treatment for 2 days, and then transfer to glycerol potassium hydroxide transparent solution for transparent treatment fo...

Embodiment 2

[0029] A method for preparing a transparent skeleton specimen of a yellow-bellied titmouse, comprising the following steps:

[0030] Step 1, take the dead yellow-bellied chickadee and fix it with 4% formaldehyde solution for 10 days, then rinse the yellow-bellied chickadee with fixed posture with running water, and then bleach it with 1% potassium hydroxide solution for 7 days;

[0031] Step 2, using 50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethanol from low concentration to high concentration to carry out gradient dehydration to the yellow-bellied tit after the fixed treatment of step one, 7 days for each concentration gradient;

[0032] Step 3, degreasing the dehydrated yellow-bellied chickadee in a sodium hydroxide solution with a mass concentration of 1% for 3-4 days, and then degreasing with xylene for 1 day;

[0033] Step 4, put the yellow-bellied tit degreased in step 3 into a potassium hydroxide solution with a mass conc...

Embodiment 3

[0039] A method for preparing a transparent skeleton specimen of a yellow-bellied titmouse, comprising the following steps:

[0040] Step 1, take the dead yellow-bellied tit and fix it with absolute ethanol for 7 days, then rinse the yellow-bellied tit with a fixed posture with running water, and then bleach it with 5% hydrogen peroxide for 7 days;

[0041] Step 2, using 50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethanol from low concentration to high concentration to carry out gradient dehydration to the yellow-bellied tit after the fixed treatment of step one, 7 days for each concentration gradient;

[0042] Step 3, degreasing the dehydrated yellow-bellied chickadee in a sodium hydroxide solution with a mass concentration of 1% for 3-4 days, and then degreasing with xylene for 1 day;

[0043] Step 4, put the yellow-bellied tit degreased in step 3 into a potassium hydroxide solution with a mass concentration of 1% for transparen...

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PUM

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Abstract

The invention relates to a method for making a transparent parusvenustulus skeleton specimen. Compared with the prior art, the dehydration gradient is increased, the gradient difference is reduced, dehydration is soft in intensity and thorough, and deformation or denaturation of the specimen can be prevented in the dehydration process. According to the method, sodium hydroxide and xylene are combined for degreasing, the degreasing effect is better and more thorough, degreasing time is short, specimen making time is shortened to a certain degree, and making efficiency is improved; a method that two kinds of transparency agents including potassium hydroxide and glycerin are used in cooperation is adopted, transparency is carried out for two days through a low-concentration potassium hydroxide solution and is soft, the specimen is not damaged, then the specimen is transferred into glycerin potassium hydroxide transparency liquid, and transparent treatment is carried out for 5 days; due to glycerin, corrosion of the specimen by potassium hydroxide is reduced, the concentration of the potassium hydroxide can be increased to be higher than 3.5%, and the transparency effect can be improved; as alcian blue dye liquor is used, cartilage is dyed into a blue color, and the transparent skeleton specimen is bright in color and more attractive.

Description

technical field [0001] The invention relates to the technical field of specimen preparation methods, in particular to a preparation method of a yellow-bellied tit transparent bone specimen. Background technique [0002] The scientific name of the yellow-bellied titmouse is Parusvenustulus. It is small in size. The head and upper back of the male bird are black, with a white patch on the cheeks and nape of the neck. The lower back and waist are bright blue-gray, and the lower chest to the undertail coverts are yellow. The upper body of the female bird is gray-green, the chin, throat, cheeks and ear feathers are gray-white, and the rest of the lower body is light yellow-green. The yellow-bellied chickadee is a special bird in China. It is distributed in the southwest of Gansu, Taibai Mountain in the Qinling Mountains in the south of Shaanxi, and Funiu Mountain in Henan. This species has been included in the "National Protected Terrestrial Wild Animals of Beneficial or Importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 李梦云刘秀英司丽芳李健孙珺李翔林霖
Owner HENAN UNIV OF SCI & TECH
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