Method for efficiently breeding tissue culture seedlings of bletilla striata and planting method of bletilla striata

Abstract

The invention provides a method for efficiently breeding tissue culture seedlings of bletilla striata and a planting method of the bletilla striata. The method for efficiently breeding the tissue culture seedlings of the bletilla striata comprises the following steps: seeding bletilla striata seeds into a seed germination culture medium; carrying out cultivation and germination for 30-40d; and transferring the germinated seeds into a seedling culture medium to cultivate for 3-4 months, wherein the seed germination culture medium comprises 1 / 2 modified MS culture medium, 30-40g / L of potato juice and 20-30g / L of cane sugar; the seedling culture medium comprises the modified MS culture medium, 30-40g / L of a potato juice, 60-70g / L of a banana juice, 20-30g / L of cane sugar and 3.8-4.5g / L of agar powder. The planting method of the bletilla striata comprises the following steps: transplanting the tissue culture seedlings of the bletilla striata into a farmland; carrying out water and fertilizer management after transplanting; and carrying out weed control and diseases and pests prevention and control. The methods are liquid culture medium methods, and only need to transfer once. Compared with a traditional solid culture method, the methods are simple to operate, small in pollution, low in cost, high survival rate and short in culture cycle.

Description

technical field [0001] The invention relates to the field of plant seedling cultivation, in particular to a method for efficiently propagating bletilla striata tissue-cultured seedlings and a method for planting bletilla striata. Background technique [0002] Bletilla striata is a perennial herbaceous bulbous plant with a plant height of 18-60 cm. It is mainly distributed in China, Japan and northern Myanmar. The main flowering period is in spring, but depending on the climate of each place, it may bloom from late winter to early summer. Bletilla striata has a wide range of medicinal value and garden value. Bletilla striata is mainly used to astringe and stop bleeding, reduce swelling and promote muscle growth. It can be used as a potted plant for indoor viewing, or as a decoration in a relatively shaded flower bed, flower border or corner of a courtyard. [0003] The capsule of Bletilla striata contains tens of thousands of fine seeds. After disinfection and sterilization,...

AI Technical Summary

Problems solved by technology

For such a tissue culture procedure, the whole process needs to be transferred 4 times, the steps are cumbersome, the operation is complicated, and the chance of contamination is increased.

Images