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Penthorum chinense pursh extract and preparing method and application thereof

An extract and extract technology of C. chinensis, which is applied in the field of C. chinensis extraction and its preparation, can solve the problems of insufficient enrichment of active ingredients, which have not been seen yet, and achieve good clinical application prospects, excellent effects, and preventive and the effect of treating liver damage

Active Publication Date: 2015-12-09
四川古蔺肝苏药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that C. chinensis has the pharmacological activity of preventing and treating hepatitis and protecting the liver, but so far there has been no report on the active ingredients of C. chinensis on the prevention and treatment of ischemia-reperfusion liver injury and chemical liver injury.
In addition, the "Gansu Granules" series of Ganhuangcaocheng prescription preparations currently in clinical use, because the production process is still the traditional water extraction and alcohol precipitation, the active ingredients are not fully enriched, and they are mostly used in clinical adjuvant treatment of liver diseases, so further research is needed. improvement

Method used

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  • Penthorum chinense pursh extract and preparing method and application thereof
  • Penthorum chinense pursh extract and preparing method and application thereof
  • Penthorum chinense pursh extract and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of the macrocyclic polyphenol extract of C. chinensis of the present invention

[0052] Crush the herb medicinal material, pass through No. 2 sieve, extract twice under reflux with 95% ethanol, each extraction time is 2 hours, add 95% ethanol V / W of 8 times the volume of the original medicinal material each time, combine the extracts, reduce Concentrate under pressure until there is no alcohol smell to obtain the crude extract extract (relative density is 1.12~1.18), the yield is 9.7%;

[0053] Add 2 times the volume of water to suspend the above-mentioned C. chinensis extract and filter it. The filtrate passes through the HPD-400 macroporous resin column. The loading volume is 2 / 3 of the volume of the macroporous resin column. Water, 10% ethanol, 30 % ethanol, 50% ethanol and 95% ethanol were eluted, and the eluent was 7 times the volume of the column to obtain water, 10% ethanol, 20% ethanol, 30% ethanol, 50% ethanol and 95% ethanol eluent respec...

Embodiment 2

[0054] Example 2 Preparation of the macrocyclic polyphenol extract of C. chinensis of the present invention

[0055] Crush the herb medicinal material, pass through No. 2 sieve, extract twice under reflux with 60% ethanol, each extraction time is 2 hours, add 60% ethanol V / W of 10 times the volume of the original medicinal material each time, combine the extracts, reduce Concentrate under pressure until there is no alcohol smell to obtain the crude extract extract (relative density is 1.12~1.18), the yield is 11.2%;

[0056] Add 2 times the volume of water to suspend the above-mentioned C. chinensis extract and filter it. The filtrate passes through the HPD-400 macroporous resin column. The loading volume is 2 / 3 of the volume of the macroporous resin column. Water, 10% ethanol, 30 % ethanol, 50% ethanol and 95% ethanol were eluted, and the eluent was 7 times the volume of the column to obtain water, 10% ethanol, 20% ethanol, 30% ethanol, 50% ethanol and 95% ethanol eluent resp...

Embodiment 3

[0057] Example 3 Preparation of the macrocyclic polyphenol extract of C. chinensis of the present invention

[0058] Crush yellow grass, pass through No. 2 sieve, add 30% ethanol and heat reflux to extract 3 times, each extraction time is 3 hours, each time add water V / W 15 times the volume of raw material weight, combine the extracts, concentrate under reduced pressure to No alcohol taste crude extract extract (relative density 1.12 ~ 1.18); yield 14.7%;

[0059] Add 1 times the volume of water to suspend the above-mentioned C. chinensis extract and centrifuge (5000r / min, 20min). The supernatant passes through the HPD-400 macroporous resin column, and the loading volume is 1 / 2 of the volume of the macroporous resin column. , sequentially eluted with water, 10% ethanol, 30% ethanol, 50% ethanol and 95% ethanol, and the eluent was 5 times the volume of the column to obtain water, 10% ethanol, 20% ethanol, 30% ethanol, 50% ethanol, respectively Ethanol and 95% ethanol eluate, t...

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Abstract

The invention provides a penthorum chinense pursh extract. The HPLC finger-print is characterized by comprising four characteristic peaks, wherein the retention time of the first peak is 10.4+ / -0.25 min, and the peak area of the first peak is 1682000+ / -8000; the retention time of the second peak is 12.5+ / -0.25 min, and the peak area of the second peak is 36750+ / -700; the retention time of the third peak is 13.0+ / -0.25 min, and the peak area of the third peak is 2461000+ / -3000; the retention time of the fourth peak is 14.9+ / -0.25 min, and the peak area of the fourth peak is 1831000+ / -5000. The treatment effect of the penthorum chinense pursh extract is excellent in treating hepatic ischemia reperfusion liver injuries and chemical liver injuries and has broad clinical application prospects.

Description

technical field [0001] The present invention relates to an extract of C. chinensis and its preparation method and use. Background technique [0002] Common liver injuries include ischemia-reperfusion liver injury and chemical liver injury. Among them, ischemia-reperfusion injury (ischemiareperfusioninjury, IRI) is one of the most common problems in clinical surgery. Ischemic injury occurs when the blood perfusion flow of tissues and organs is reduced due to various reasons, and after the blood perfusion is restored, the cell function metabolism and structural damage are aggravated, resulting in ischemia-reperfusion injury. Today, as the technology of liver transplantation is becoming more and more mature, after the transplanted liver undergoes ischemic injury, with the opening of the blood, it will experience the second blow of reperfusion injury. Asymptomatic, mild abnormal liver function to liver failure, etc.), but more and more studies have shown that ischemia-reperfus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P1/16
Inventor 江云陈万生孙连娜张大永黄豆豆兰泽伦刘静
Owner 四川古蔺肝苏药业有限公司
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