Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nandina domestica fire power primary culture medium

A technique for cultivating Nandina japonica and the first generation, which is applied in the fields of horticulture, botanical equipment and methods, gardening tools/equipment, etc. It can solve the problems of slow cultivation speed and low multiplication coefficient, so as to increase the number of germinations, simplify the cultivation process, and improve the production of hormones. Reasonable effect

Active Publication Date: 2015-12-09
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the relevant literature on the tissue culture of flame nandina that has been published so far, the proliferation coefficient of tissue culture rapid propagation is low and the culture speed is slow, so it is particularly important to carry out the primary culture of flame nandina

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nandina domestica fire power primary culture medium
  • Nandina domestica fire power primary culture medium
  • Nandina domestica fire power primary culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Medium formula: improved MS+zeatin (0.6mg / L)+indole acetic acid (0.2mg / L)+glucose (15g / L)+agar powder (6g / L).

[0021] The formula for the improved MS is:

[0022] Macroelements: (NH 4 ) 2 SO 4 600mg / L, CO(NH 2 )500mg / L, KNO 3 950mg / L, CaCl 2 2H 2 O220mg / L, MgSO 4 ·7H 2 O185mg / L, KH 2 PO 4 85mg / L.

[0023] Trace elements: KI0.41mg / L, H 3 BO 3 3.1mg / L, MnSO 4 4H 2 o 2 1.15mg / L, ZnSO 4 ·7H 2 O4.3mg / L, Na 2 MoO 4 2H 2 O0.125mg / L, iron salt: FeSO 4 ·7H 2 O(27.8), Na 2 -EDTA·2H 2 O(37.3).

[0024] Organic substances: inositol 50mg / L, niacin 0.5mg / L, pyridoxine hydrochloride (vitamin B6) 0.5mg / L, thiamine hydrochloride (vitamin B1) 0.1mg / L, calcium pantothenate (vitamin B5) 1mg / L L, vitamin C 2mg / L, glycine 2.0mg / L.

[0025] Add each material according to the amount required by the above medium formula, and boil to fully mix and dissolve all the materials. Adjust the pH of the culture medium to 5.7, pack in required containers and sterilize at high...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a medium formula which is most suitable for nandina domestica fire power primary culture. The medium formula comprises modified MS+zeatin (0.6mg / L)+indole acetic acid (0.2mg / L)+glucose (15g / L)+agar powder (6g / L). The hormone combination in the medium of the invention is reasonable, primary culture and rooting are combined in one step, stem sections can take roots in a primary culture process, the culture process is simplified, and the culture time is shortened. Meanwhile, a bud number of primary culture plants can be improved, the plants grow robustly, which is conductive to next step transgenerational propagation, the medium can provide more high quality seedlings for the nandina domestica fire power, amd is conducive to widespread promotion, and greater economic benefits are created.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, in particular to the rapid propagation technology of flame nandina tissue culture. Background technique [0002] Flame nandina is a new type of greening seedlings. Its leaves are bright and bright like fire in winter, and are very popular among the people. Because the flame nandina plants are short, the stem nodes are short, the internodes are only 1 / 10 of ordinary nandina, and the branches are thin and weak, so cutting propagation is difficult and the propagation speed is slow. Since the Shanghai Botanical Garden introduced this species as a collection of resources in 1995, the research on flame nandina in my country has only begun. The cutting method is usually used in production, which has a long reproduction cycle and extremely low reproduction rate, and cannot achieve good results in production. With the continuous development of tissue culture technology in recent years, especially ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 贾思振张立伟王媛花颜志明
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com