Method for inducing Corsvenor Momordica Fruit parthenocarpy by using hormone

A technology of parthenocarpy and Luo Han Guo, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of not screening out parthenocarpic mutant varieties, unreasonable male and female ratio, and the need for artificial pollination, etc., to achieve Small cost, low equipment requirements, good appearance effect

Inactive Publication Date: 2015-12-09
怀化兴科创生物技术有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Luo Han Guo is a dioecious plant. Its sticky pollen is difficult to be pollinated by insect vectors and wind vectors. When planting, it needs to be equipped with male plants and manual pollination is required, which requires a lot of manpower and material resources.
And if the male and female do not meet at the flowering period or the ratio of male and female is un

Method used

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  • Method for inducing Corsvenor Momordica Fruit parthenocarpy by using hormone
  • Method for inducing Corsvenor Momordica Fruit parthenocarpy by using hormone
  • Method for inducing Corsvenor Momordica Fruit parthenocarpy by using hormone

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, preparation of different concentrations of hormones

[0027] In order to test the influence and effect of different concentrations, different types and different ratios of hormones on the parthenocarpic induction of Luo Han Guo, the present invention will IAA, 2,4-D, 6-BA, GA3, KT, CPPU, 2,4-D+ GA3, 6-BA+GA3, KT+GA3, CPPU+GA3 and other hormones were prepared according to the concentrations in Table 1 below.

[0028] Table 1: Types and concentrations of hormones used in the present invention

[0029]

[0030]

Embodiment 2

[0031] Embodiment 2, Momordica grosvenori parthenogenesis induction experiment

[0032] This experiment was carried out twice, respectively in Changde, Hunan and Shenzhen, Guangzhou.

[0033] The specific experimental methods and steps are as follows:

[0034] a) The female plant of the test material is planted in the field according to the conventional planting method of Luo Han Guo, and 90-150 plants are planted per mu;

[0035] b) During the flowering stage, different parts of the female flowers of Luo Han Guo were treated with different hormone concentrations and hormone ratios prepared in Example 1. In the present invention, three different parts of Luo Han Guo are treated, and the parts include the ovary wall and stigma of female flowers, or the ovary wall and stigma are subjected to hormone treatment at the same time; the treatment period is the day before flowering, the day of flowering and the day after flowering ; The different hormones are CPPU, 2,4-D, IAA, 6-BA, GA...

Embodiment 3

[0037] Embodiment 3, statistical analysis of data

[0038] After harvesting the fruit, the results of the statistical experiment (Table 2) were analyzed and the following conclusions were drawn: Both the hormone CPPU and 2,4-D have the effect of inducing parthenocarpy of Luo Han Guo, whether used alone or in combination with other hormones GA3 , but the time of hormone treatment needs to be the day of flowering, neither the day before flowering nor the day after flowering can induce parthenocarpy (see Table 3), and the part of hormone treatment is the ovary wall, and the application of hormones on the stigma not only does not increase the size of the fruit , There is also the risk of increasing deformed fruit (Table 4).

[0039] According to statistics, in the fruit of parthenocarpic induction, when the concentration of CPPU or 2,4-D is 10 mg / L, and combined with 20 mg / L GA3, the best effect can be obtained. The fruit is square and the size reaches the standard of medium-size...

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Abstract

The invention relates to a method for inducing Corsvenor Momordica Fruit parthenocarpy by using hormone. The method is characterized in that a combination of different hormones is applied to Corsvenor Momordica Fruit in different flowering phases in order to obtain the stable and effective parthenocarpy inducing method. The method omits tedious pollination work, and allows seedless Corsvenor Momordica Fruit convenient to process to be obtained. Compared with traditional triploid seedless Corsvenor Momordica Fruit preparation methods, the method has the advantages of no need of chromosome doubling or hybridization, short production cycle, simplicity, easy operation, and suitableness for large-area application.

Description

technical field [0001] The invention relates to a method for inducing parthenocarpy of Luo Han Guo by means of exogenous hormones, specifically, a method for finally obtaining parthenocarpic seedless Luo Han Guo by treating the ovary wall of female flowers of Luo Han Guo with different hormones. Background technique [0002] Luo Han Guo is a perennial herbaceous vine of Cucurbitaceae. Mangosteen is abundant in the mountainous area of ​​Yongfu County, Guilin City. It is a precious medicinal material, a high-end refreshing drink and a good condiment. Its effect of clearing the lungs and relieving the throat has become highly sought after due to the recent serious smog pollution. [0003] Parthenocarpy is the phenomenon in which an ovary forms a seedless fruit without fertilization. The parthenocarpic fruit is a real seedless fruit, which is convenient to eat and easy to process. The unique sweet glycosides in Luo Han Guo have high sweetness and low calorie value, and are id...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 张会新张蕾夏勉石九英
Owner 怀化兴科创生物技术有限公司
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