Method for identifying donkey skin from counterfeit species by applying mitochondria COI (cytochrome oxidase subunit I) sequence segments and specific primer for amplifying segments

Abstract

The invention discloses a method for identifying donkey skin from counterfeit species by applying mitochondria COI (cytochrome oxidase subunit I) sequence segments and a specific primer for amplifying the segments, belonging to the technical field of identification of Chinese medicine. According to the method disclosed by the invention, COI sequences of the donkey skin and the counterfeit species are amplified by using an autonomously designed primer, the donkey skin is distinguished from pigskin or cowhide by judging whether a PCR (Polymerase Chain Reaction) product exists in an amplified band in gel electrophoresis or not, and mitochondria COI sequences of the donkey skin from multiple sources and common counterfeit species are subjected to sequence assembly and compared, and then the donkey skin and counterfeit species thereof are intuitively identified through a K2P distance method. The method disclosed by the invention is convenient and rapid and high in accuracy and has the capability of well ensuring the quality of donkey-hide gelatin and donkey skin medicinal materials.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicinal materials, in particular to a method for identifying donkey hides and counterfeit products using mitochondrial COI sequence fragments and amplifying specific primers thereof. Background technique [0002] Donkey skin is the main raw material for making donkey-hide gelatin, but due to the shortage of supply, fake skins often appear in the market. Common fake hides are mainly horse hides and mule hides. Horse hides can kill insects and relieve itching. Mule hides are not used for medicine. Once mixed with donkey hides, the quality of donkey-hide gelatin will be seriously affected. Donkey skin is not listed in the current national pharmacopoeia. In order to avoid mixing with other animal skins, "Shandong Province Traditional Chinese Medicine Standards" (2010 Edition) stipulates that donkey skin must be the whole dry skin or fresh skin of a donkey, and the broke...

AI Technical Summary

Problems solved by technology

There are few reports on the authenticity identification of animals in Chinese herbal medicines [114] , there is no relevant research report on using COI sequence to identify donkey skin and its counterfeit products

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