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Novel stem cell preparation and application thereof in vascular intervention therapy of cerebral apoplexy

A technology of stem cell preparation and interventional therapy, which is applied in the field of biotechnology and regenerative medicine, can solve the problems of low degree of differentiation of neural stem cells into nerve cells and the need to further improve the treatment effect of patients, and achieves easy collection and transportation, low preparation cost, The effect of a large number of cells

Active Publication Date: 2015-12-02
奥思达干细胞有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the main problem faced by the use of stem cell transplantation in the treatment of stroke is that the degree of differentiation of the transplanted neural stem cells into nerve cells is still low, and the therapeutic effect on patients needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Extraction and preparation of human umbilical cord stem cells

[0045] The extraction method is as follows:

[0046] 1. The umbilical cords of newborns delivered by cesarean section from healthy pregnant women were taken, and the donors were tested negative for hepatitis virus markers, syphilis, and HIV. The donor gave informed consent to the experiment, which was approved by the ethics committee of the hospital.

[0047] 2. Under sterile conditions, remove the umbilical cord of the newborn by cesarean section from the operating table, immerse it in F12 medium, and store it at 4°C; take out the umbilical cord from the medium in the ultra-clean bench, containing 1× double antibody and 250 μg Rinse the outer surface of the umbilical cord with D-Hank'S solution of / ml amphotericin, and insert a 20ml syringe into two umbilical veins and one umbilical artery for repeated washing to remove the remaining blood in the umbilical vein and artery.

[0048] 3. Cut the...

Embodiment 2

[0057] Example 2: Preparation of stem cells and stem cell preparations for treating stroke

[0058] In this example, on the basis of Example 1, the extracted stem cells were further cultured, induced to differentiate and identified.

[0059] 1. Take P4-P6 generation umbilical cord stem cells. When the cells adhere to the wall and grow to 80-90%, absorb the original culture medium in the culture bottle, take 10ml0.01MPBS and gently add it into the culture bottle to wash twice, discard the washing liquid .

[0060] 2. Add 1.0ml of 0.025%~0.05% trypsin-0.01%EDTA digestion solution, soak to cover the bottom of the bottle, and when the cells are observed under an inverted microscope to float in a round shape, add 1.0ml of FBS to stop the trypsin digestion.

[0061]3. Add 10ml of 0.01MPBS to wash repeatedly by pipetting, and centrifuge at 900rpm for 10 minutes at room temperature; 2 Inoculate 2×10 5 stem cells.

[0062] 4. The culture system is F12 complete culture medium cont...

Embodiment 3

[0072] Example 3: Observing the repairing effect of stem cell therapy on the limb function of patients

[0073] One of the important attributes of stem cells is the ability to repair and regenerate damaged tissues in the body. We adopted the method of interventional therapy, injected the femoral artery into the patient, and transplanted it every 7 days. After 3 consecutive transplants, the limb function was significantly improved compared with the control group.

[0074] Adopt 47 patients with cerebral apoplexy, one month after treatment, 10 experimental group patients injected with stem cell preparations of the present invention, 8 patients of control group 1 injected with EGF stem cell preparations, 10 patients of control group 2 injected with EGF and CTP stem cell preparations, The European Stroke Scale (ESS) and life function score (BI) of the 9 patients in the control group 3 who were injected with EGF and LiCl stem cell preparations and the 10 patients in the blank gro...

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Abstract

The invention discloses a novel stem cell preparation and application thereof in vascular intervention therapy of cerebral apoplexy and provides a preparation method and steps of stem cells in the stem cell preparation and their application in the preparation of pharmaceuticals used for treating patients with cerebral apoplexy. The novel stem cell preparation has a good effect after transplanting, preparation cost is low, acquired cells are easy to massively amplify, new fields are opened for the treatment of nervous system diseases such as cerebral apoplexy, and good news are brought to the patients with cerebral apoplexy. The stem cells in the preparation are higher in differentiation degree, is capable of promoting neural functional recovery of patients during vascular intervention therapy of cerebral apoplexy, enables higher cell colonization, is easy to prepare and convenient to apply, and has curative effect evidently higher than like drugs.

Description

technical field [0001] The invention belongs to the field of biotechnology and regenerative medicine, in particular to a novel stem cell preparation and its use in vascular interventional treatment of stroke. Background technique [0002] Stroke is a sudden-onset cerebral blood circulation disorder disease, which is the second cause of death and the first cause of disability in the world, and about half of the survivors are permanently disabled. According to data from the Chinese Center for Disease Control and Prevention, stroke is also the first cause of disability and death in my country. There are more than 7 million stroke patients in my country, and the incidence rate increases by 8.7% every year. About 1.6 million people die of stroke every year, and the mortality rate is 157 / 100,000 people. Similar to other countries, ischemic stroke is the most common type of stroke in my country, accounting for about 43-79% of all strokes. Therefore, stroke is one of the major pub...

Claims

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Application Information

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IPC IPC(8): A61K35/51C12N5/074A61P9/10
Inventor 周萱王云娟尤丹丹
Owner 奥思达干细胞有限公司
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