Application of soracan gum in hair washing and caring
A sola gel and shampoo technology, which is applied in the field of cosmetics and medicine, can solve irritation and other problems, and achieve the effect of promoting hair growth, fast speed and short cycle
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Embodiment 1
[0021] Preparation of crude Sola gum: configure culture medium, weigh 1gNaH 2 PO 4 , 3gKNO 3 , 0.07gCaCl 2 , 0.3gMgSO 4 ·7H 2 O, 0.0125g FeSO 4 ·7H 2 O, 0.003gMnSO 4 , 0.0075gZnCl 2 , 20g sucrose, adding H 2 O to a volume of 1000 mL and adjust the pH to 7. The culture solution was sterilized by heating at 121°C for 20 minutes, cooled to room temperature, inoculated with 200 μL of colony solution, and incubated at 28°C for 12 hours to form a seed solution. Add 5% seed culture solution to the sterilized (sterilized at 121° C. for 20 minutes) culture solution, and ferment at 28° C. for 48 hours. Two times the volume of 95% ethanol was added to the fermentation broth to precipitate polysaccharides, and the precipitate was vacuum-dried at 50° C. to obtain crude sola gum.
Embodiment 2
[0023] Preparation of Sola gum solution: The crude Sola gum can be purified by the improved Sevag method. The purification steps are as follows:
[0024] a. Prepare 0.5% Sola gum solution, heat (105°C, 15min), and when cooled to room temperature, 7000r / 10min, take the supernatant.
[0025] b. Add pH4.0 acetic acid---sodium acetate solution at a ratio of 1:30 to remove DNA.
[0026] c. Mix the supernatant with phenol chloroform solution (prepared as a mixed solution at a ratio of 1:1) at a ratio of 1:1, shake vigorously for about 15 minutes, 7000r / 10min, and take the supernatant. Repeat this step 2-3 times to completely remove the protein.
[0027] d. Add 2 times the volume of industrial alcohol to the supernatant, the sugar flocculates into a ball, remove it, and squeeze out the alcohol.
[0028] e. Wash the precipitate with 75% alcohol for 2-3 times, and then wash the precipitate with 95% alcohol for 1-2 times.
[0029] f. Vacuum freeze drying.
Embodiment 3
[0031] On the basis of the above-mentioned technical scheme, it is said that the obtained Sola gum is dissolved in distilled water at 1% by mass percentage, and after being completely dissolved, it is added to the semi-finished product of shampoo or conditioner, stirred evenly, and the final concentration is 0.5%, that is to get the final finished shampoo or conditioner.
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