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Camellia oleifera pollen ultra low temperature storage method

A technology for ultra-low temperature preservation and Camellia oleifera pollen, which is applied in the fields of plant preservation, botanical equipment and methods, application, etc., can solve the problems of unfavorable Camellia oleifera germplasm resources research, hybrid breeding, difficulty in research on Camellia oleifera hereditary traits, and short lifespan of Camellia oleifera pollen. The ultra-low temperature preservation method of Camellia oleifera pollen is simple and easy to operate, overcomes the difficulties of hybrid breeding, and the drying time is short.

Inactive Publication Date: 2015-12-02
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Camellia oleifera is a self-incompatible plant, and the flowering period between species does not meet. Under natural conditions, the pollen life of camellia oleifera is short, which is not conducive to the research of camellia oleifera germplasm resources and its hybrid breeding, and also brings many difficulties to the study of camellia oleifera genetic traits.

Method used

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  • Camellia oleifera pollen ultra low temperature storage method
  • Camellia oleifera pollen ultra low temperature storage method
  • Camellia oleifera pollen ultra low temperature storage method

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: A method for ultra-low temperature preservation of camellia pollen, including the following steps:

[0025] Step (1) Dehydration treatment: The thermostat is adjusted to a constant temperature of 30°C, and the collected 500mg of "Changlin No. 4" Camellia oleifera fresh pollen is evenly spread on the sulfuric acid paper, placed in the thermostat, and dried to a moisture content of 13.48% ;

[0026] Step (2) Pre-freezing treatment: Pre-freeze the dehydrated "Changlin No. 4" Camellia pollen at -26℃ for 1h, 4℃ for 1h, 4℃(1h)→-26℃(1h)→- 80℃ (1h) gradually lower the temperature and pre-freeze or not, put it into liquid nitrogen for storage;

[0027] Step (3) Thawing treatment: After storing "Changlin No. 4" Camellia pollen in liquid nitrogen for 30 days, thaw it in a 38 ℃ water bath for 3 minutes, measure the pollen viability, and determine the best pre-freezing method for "Changlin No. 4" Camellia pollen:- Pre-freeze at 26°C for 1h.

[0028] Table 1 "Changlin No. 4" Cam...

Embodiment 2

[0031] Step (1) Dehydration treatment: the thermostat is adjusted to a constant temperature of 30°C, and the collected 500mg of "Gan 5" Camellia oleifera fresh pollen is evenly spread on sulfuric acid paper, placed in the thermostat, and dried to a moisture content of 14.17%;

[0032] Step (2) Pre-freezing treatment: pre-freeze the dehydrated "Gan 5" Camellia pollen at -26℃ for 1h, 4℃ for 1h, 4℃(1h)→-26℃(1h)→-80℃ (1h) Pre-freezing and non-pre-freezing are gradually lowered and stored in liquid nitrogen;

[0033] Step (3) Thawing treatment: "Gan 5" Camellia pollen is stored in liquid nitrogen for 30 days, and then thawed in a 38℃ water bath for 3 minutes. The viability of the pollen is measured and the best pre-freezing method for "Gan 5" Camellia pollen is -26℃ for 1 hour. .

[0034] Table 2 "Gan 5" Camellia pollen was pre-frozen by different pre-freezing methods, and the pollen germination rate after 30 days of ultra-low temperature storage.

[0035] Pre-freezing method

Embodiment 3

[0037] Step (1) Dehydration treatment: The thermostat is adjusted to a constant temperature of 30°C, and the collected 500mg of "91" Camellia oleifera fresh pollen is evenly spread on the sulfuric acid paper, placed in the thermostat, and dried to a moisture content of 15.52%;

[0038] Step (2) Pre-freezing treatment: Pre-freeze the dehydrated "91" Camellia pollen at -26℃ for 1h, 4℃ for 1h, 4℃(1h)→-26℃(1h)→-80℃( 1h) Pre-freezing and non-pre-freezing are gradually lowered and stored in liquid nitrogen;

[0039] Step (3) Thawing treatment: After storing "No. 91" camellia pollen in liquid nitrogen for 30 days, thaw it in a 38℃ water bath for 3 minutes, measure the pollen viability, and determine the best pre-freezing method for "No. 91" camellia pollen at -26°C for 1 hour .

[0040] Table 3 "No. 91" camellia pollen was pre-frozen by different pre-freezing methods, and the pollen germination rate after 30 days of ultra-low temperature storage.

[0041] Pre-freezing method

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Abstract

The present invention relates to a camellia oleifera pollen ultra low temperature storage method, which comprises: 1, dewatering: placing collected camellia oleifera pollen into a constant temperature box to dry; 2, pre-freezing: pre-freezing the obtained camellia oleifera pollen; 3, freezing: pouring the pre-frozen camellia oleifera pollen into liquid nitrogen so as to be stored; and 4, thawing: thawing the stored camellia oleifera pollen. According to the present invention, with the camellia oleifera pollen ultra low temperature storage method, the pollen aging can be effectively inhibited, the respiration rate and the enzyme activity can be reduced, the camellia oleifera pollen life can be prolonged, the cross breeding difficulty caused by the non-meeting flowering seasons among the camellia oleifera species can be overcome, the vitality is up to 47.05% after the camellia oleifera pollen is stored in liquid nitrogen for six months, the drying time is short, the pollen viability is ensured, the storage is easily achieved, the drying method input cost, the pre-freezing cost and the thawing cost are low, the operation is simple, the hybridization viability of the camellia oleifera pollen can be effectively ensured, and the important promotion value is provided for the camellia oleifera cross breeding and production practice.

Description

Technical field [0001] The invention relates to a preservation method of plant pollen, in particular to an ultra-low temperature preservation method of camellia pollen. Background technique [0002] Camelliaoleifera belongs to the Camellia family (Theaceae). It is an evergreen shrub or small tree. It has strong adaptability, tolerance to barrenness and drought. It is one of the unique woody oil tree species in my country and the main source of red soil transformation. Tree species, known as the "Oriental Olive Tree" by the West. Camellia oleifera has been listed as a key edible oil tree species developed in my country. The tea oil has a clear taste, rich nutrition, and contains natural vitamins A and E. It is storage-resistant and easily absorbed by the human body. It can prevent and treat high blood pressure and cardiovascular diseases. It is a green health-care edible oil. In summary, Camellia oleifera has both high ecological and economic value. [0003] Pollen, as one of the ...

Claims

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Application Information

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IPC IPC(8): A01N3/00
Inventor 姚小华龙伟常维霞
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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