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Method for detecting p-nitrophenol based on molecular imprinting ratio type fluorescent probe

A technology of p-nitrophenol and molecular imprinting, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., and can solve the problem of reducing the selectivity of the detection process

Active Publication Date: 2015-11-25
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This probe based on a single fluorescence intensity change is not only susceptible to the influence of the detection substrate, photobleaching and other shortcomings, but also may be interfered by fluorescent quenchers such as heavy metal ions and active oxygen radicals at the same time, which reduces the detection process. selectivity

Method used

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  • Method for detecting p-nitrophenol based on molecular imprinting ratio type fluorescent probe
  • Method for detecting p-nitrophenol based on molecular imprinting ratio type fluorescent probe
  • Method for detecting p-nitrophenol based on molecular imprinting ratio type fluorescent probe

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Effect test

Embodiment 1

[0025] A method for detecting p-nitrophenol based on molecularly imprinted ratiometric fluorescent probes, the steps are as follows:

[0026] (1) Preparation of fluorescent silica nanoparticles coated with quantum dots;

[0027] Fluorescent silica nanoparticles coated with quantum dots are prepared by adopting the methods of published Chinese patents (authorized notification numbers: CN102757790B and CN1306002C).

[0028] (2) Preparation of siloxane functionalized carbon dots;

[0029] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0030] (3) Preparation of core-shell dual-emission fluorescent silica nanoparticles;

[0031] Take 5 mg of fluorescent silica nanoparticles coated with quantum dots obtained in step (1) and ultrasonically disperse them in 50 mL of anhydrous toluene, add 5 μL of siloxane functionalized carbon dots obtained in step (2), stir evenly, and heat to Reflux te...

Embodiment 2

[0037] (1) Preparation of fluorescent silica nanoparticles coated with quantum dots;

[0038] Fluorescent silica nanoparticles coated with quantum dots are prepared by adopting the methods of published Chinese patents (authorized notification numbers: CN102757790B and CN1306002C).

[0039] (2) Preparation of siloxane functionalized carbon dots;

[0040] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0041] (3) Preparation of core-shell dual-emission fluorescent silica nanoparticles;

[0042]Take 5 mg of fluorescent silica nanoparticles coated with quantum dots obtained in step (1) and ultrasonically disperse them in 100 mL of anhydrous toluene, add 15 μL of the siloxane functionalized carbon dots obtained in step (2), stir evenly, and heat to Reflux temperature, after 16 hours of reaction, the product was centrifuged to obtain 5.9 mg of solid powder of core-shell dual-emission flu...

Embodiment 3

[0048] (1) Preparation of fluorescent silica nanoparticles coated with quantum dots;

[0049] Fluorescent silica nanoparticles coated with quantum dots are prepared by adopting the methods of published Chinese patents (authorized notification numbers: CN102757790B and CN1306002C).

[0050] (2) Preparation of siloxane functionalized carbon dots;

[0051] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0052] (3) Preparation of core-shell dual-emission fluorescent silica nanoparticles;

[0053] Take 5 mg of fluorescent silica nanoparticles coated with quantum dots obtained in step (1) and ultrasonically disperse them in 75 mL of anhydrous toluene, add 10 μL of siloxane functionalized carbon dots obtained in step (2), stir evenly, and heat to Reflux temperature, after 12 hours of reaction, the product was centrifuged to obtain 5.8 mg of solid powder of core-shell dual-emission fluores...

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Abstract

The invention relates to a method for detecting p-nitrophenol based on a molecular imprinting ratio type fluorescent probe. The molecular imprinting ratio type fluorescent probe is composed of a core-shell type double-emissivity molecular imprinting silicon dioxide nanoparticle, a quantum-dot-covering silicon dioxide nanoparticle and a siloxane functionalized carbon dot are employed for preparing the double-emissivity fluorescent nanoparticle, and then a silicon dioxide layer containing an imprinting molecule p-nitrophenol is grown on the surface of the double-emissivity fluorescent nanoparticle , so that the molecular imprinting double fluorescent composite nanoparticle is obtained. In the structure, the quantum dot in the silicon dioxide nanoparticle shell is taken as a reference fluorescent signal, and the carbon dot in the outer molecular imprinting layer is taken as a response fluorescent signal and is used for selective identification on p-nitrophenol. The method fully gives play to the advantages that molecular imprinting is resistant to interference, high in selectivity, high in sensitivity and the like, overcomes the disadvantage that a conventional single-fluorescent-signal analysis method is easily influenced by detection substrate concentration, external environment and other factors, and possesses significant meaning in the fields such as environment pollutant monitoring and control, and the like.

Description

technical field [0001] The invention relates to a fluorescence analysis and detection method for p-nitrophenol, in particular to a method for detecting p-nitrophenol based on a molecular imprinting ratio fluorescent probe. Background technique [0002] The pollution of phenol and phenolic compounds to water has attracted widespread attention, and my country has listed it as one of the pollutants that need to be controlled first in water. Among them, p-nitrophenol, as an important chemical raw material, is widely used in fine chemical industries such as explosives, dyes, and pharmaceutical intermediates, and is a class of highly toxic and refractory compounds. Because of its extensive use, a large amount of wastewater is produced, which not only pollutes the environment but also endangers biological safety. In order to ensure human health, the concentration of p-nitrophenol in water should be lower than 70 μg / L, so it is very important to accurately detect the content of p-n...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 苏荣欣高召黄仁亮齐崴王梦凡
Owner TIANJIN UNIV
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