Exosome extraction kit and application thereof in liquid biopsy of diseases including tumors

An exosome and solution technology, applied in the field of molecular biology, can solve the problems of difficult extraction and easy degradation of RNA, and achieve the effect of optimizing a simple liquid diagnosis method

Active Publication Date: 2015-11-25
骞和生物科技(广州)有限公司
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Problems solved by technology

Therefore, some researchers have tried to extract RNA from biological samples such as serum and saliva for early diagnosis, screening and diagnosi

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  • Exosome extraction kit and application thereof in liquid biopsy of diseases including tumors
  • Exosome extraction kit and application thereof in liquid biopsy of diseases including tumors
  • Exosome extraction kit and application thereof in liquid biopsy of diseases including tumors

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Embodiment Construction

[0045] Malignant tumor is one of the diseases that seriously threaten human health, and it is one of the three major causes of death in human diseases, accounting for a quarter of all disease deaths.

[0046] Esophageal cancer is one of the most common tumors of the digestive system. According to the report of the World Health Organization (WHO), 400,000 patients died of esophageal cancer worldwide in 2012, ranking sixth among tumors. In 2014, the mortality rate of esophageal cancer in my country ranked fourth among malignant tumors in my country. The symptoms of early esophageal cancer are not obvious, and most patients are approaching the middle and late stages when they are discovered or when clinical symptoms appear. Although the diagnosis and treatment of esophageal cancer have improved with the development of scientific and medical technology, the 5-year survival rate is still low. The 5-year survival rate of early esophageal cancer can reach more than 80% after surgery ...

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Abstract

The invention relates to a method and a kit for extracting RNA and application of RNA. The method comprises the following steps: (S1) centrifuging a biological sample to obtain supernate; (S2) adding an exosome precipitation solution into the supernate, and carrying out uniform mixing and centrifugation, so as to obtain exosome precipitates; (S3) adding a total RNA extraction reagent and isometric alcohol into the exosome precipitates, acutely mixing, and adding the mixture into an adsorption column; (S4) centrifuging the adsorption column, adding RNA pre-washing liquid, RNA cleaning buffer liquid and deionized water, and carrying out repeated flushing and centrifugation, so as to obtain total RNA. The invention further relates to application of fused RNA and other tumor-specific RNA extracted from exosome of body fluid in aspects of early diagnosis, screening and diagnosis of tumors. Fused RNA and other tumor-specific RNA are extracted from the exosome and are applied to the early diagnosis and screening of malignant tumors such as esophagus cancer and breast cancer, thereby simply realizing the cancer liquid diagnosis with the characteristics of little wound, good patient compliance and convenience in collection and storage.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for extracting RNA from biological samples, a kit and the application of the RNA extracted by the method. Background technique [0002] Because biological samples such as blood and saliva can reflect the physiological and pathological state of the whole body. Liquid diagnosis using serum saliva as an example has the following advantages: 1. Small trauma and good patient compliance; 2. Convenient collection and storage; 3. Simple, convenient and easy to operate, and low cost. Therefore, some researchers have tried to extract RNA from biological samples such as serum and saliva for early diagnosis, screening and diagnosis and treatment of tumors. Therefore, a method that can conveniently and accurately extract RNA from biological samples is needed. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for ...

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Application Information

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IPC IPC(8): C12N15/10C12Q1/68A61K48/00A61K31/713A61P35/00
Inventor 张灏
Owner 骞和生物科技(广州)有限公司
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