Mixed inoculant agent for effective degradation of mushroom bran, and preparation method and application thereof

A technology of mixing bacterial agents and degrading bacteria is applied in the field of degradation of edible fungi industrial waste, which can solve the problems of high crude fiber content and difficult degradation, and achieve the effects of obvious degradation effect, reduced crude fiber content and convenient operation.

Active Publication Date: 2015-11-25
ZHEJIANG NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the fungus chaff feed has not been popularized and utilized on a large scale, because the content of crude fiber in the fungus chaff is high and it is difficult to degrade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1) Aspergillus niger strains and Penicillium (both purchased from Yiyuan Kangyuan Biotechnology Co., Ltd.) were respectively inserted on the PDA slant medium and cultured for 3 days, and the spores on the slant were washed with sterile water to make a spore suspension , viable count 10 6 CFU / mL; Aspergillus niger and Penicillium were fermented and cultured in liquid at a ratio of 20% for 7 days to obtain a mixed fermentation broth;

[0018] 2) Prepare the medium first. The raw material formula of the medium is: brown sugar 100g, sterile water 1000mL; add 5g of straw starter (purchased by Nongfukang Biotechnology Co., Ltd.) into a plastic bucket containing 1000mL of medium, seal it, After 3 days of anaerobic fermentation under the condition of ℃, the straw fermentation liquid can be obtained;

[0019] 3) Take 0.5g of the special bacterial agent for crude fiber degradation (purchased from Guangzhou Nongguan Biotechnology Co., Ltd.), add it to a plastic bucket containing ...

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Abstract

The present invention relates to a mixed inoculant agent for effective degradation of mushroom bran. The mixed inoculant agent is prepared by mixing a straw fermentation broth with a crude fiber degrading broth by 1:5-5:1 to prepare a mixed inoculant stock solution, then inoculating 0.25%-1.0% of the mixed stock solution into sterile distilled water, adding a mixed broth containing Aspergillus niger and Penicillium and accounting for 1.0-5.0 wt.% of the sterile distilled water, and stably culturing for 2-3 d. The preparation method of the mixed broth is as follows: inoculating Aspergillus niger and Penicillium to a PDA slant medium for 3-4 d respectively, washing the spores on the slant with sterile water to prepare a spore suspension with viable cell count of 106 CFU / mL; and conducting liquid fermentation culture on the Aspergillus niger and Penicillium according to the proportion of 15%-25% for 7-8 d to obtain a mixed fermentation broth. The mixed inoculant agent of the present invention to is convenient for the mushroom bran degradation, and has obvious degradation effect.

Description

technical field [0001] The invention relates to the field of degradation of edible fungus industrial waste, in particular to a mixed bacterial agent for effectively degrading fungus chaff and its preparation method and application. Background technique [0002] Mushroom chaff, also known as fungus residue, refers to the culture medium waste produced after the cultivation of edible fungi, mainly including cellulose components such as cotton shells, sawdust and straw. In recent years, with the continuous expansion of my country's edible fungi industry, people's demand for edible fungi has continued to increase, which has led to an increase in the output of fungus chaff. If the fungus chaff is discarded or burned at will, it will easily cause environmental pollution and waste of resources. Therefore, the rational development and utilization of fungus chaff is very important to the recycling of cellulose resources and the reduction of environmental pollution. The fungus chaff ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/00A23K1/14C12R1/685C12R1/80
CPCY02P60/87
Inventor 蔡妙珍张丽影邢承华潘婷陈碧春
Owner ZHEJIANG NORMAL UNIVERSITY
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