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Centipede analgesic peptide SLP_RhTx and gene and application thereof

A technology of pain polypeptide and centipede, which is applied in the field of biomedicine to achieve the effect of simple structure, strong analgesic activity and convenient artificial synthesis

Inactive Publication Date: 2015-11-25
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, researchers have not yet been able to discover an inactivator that can selectively block one of the TRPV1-gated pathways

Method used

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  • Centipede analgesic peptide SLP_RhTx and gene and application thereof
  • Centipede analgesic peptide SLP_RhTx and gene and application thereof
  • Centipede analgesic peptide SLP_RhTx and gene and application thereof

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Embodiment Construction

[0032] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0033] Separation and purification of centipede analgesic peptide SLP_RhTx:

[0034] I.SephadexG-50 gel filtration chromatography:

[0035]Dissolve 200mg of freeze-dried centipede venom in 20ml of 0.1M phosphate buffer (pH6.0), centrifuge at 12,000rpm for 10 minutes, take the supernatant and use a balanced SephadexG-50 gel filtration column (26×100cm) for preliminary separation Purify, elute with the same buffer, and collect with an automatic fraction collector, the flow rate is 3ml / tube / 10min, detect the concentration of protein or polypeptide in the collected solution by ultraviolet light at 280nm, combine the fractions with analgesic activity, freeze-dry,- Store at 20°C for later use.

[0036] II. Reverse High Pressure Liquid Chromatography (RP-HPLC):

[0037] Redissolve the active peak obtained by SephadexG-50...

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Abstract

The invention discloses centipede analgesic peptide SLP_RhTx and a gene and application thereof. The centipede analgesic peptide SLP_RhTx is composed of an amino acid sequence shown in SEQ ID NO: 1. The gene sequence which encodes the centipede analgesic peptide SLP_RhTx is shown in SEQ ID NO: 2. The centipede analgesic peptide SLP_RhTx is applied as a TRPV1 channel activator to mediate a TRPV1 desensitizing drug. The centipede analgesic peptide SLP_RhTx mediates inactivation of a TRPV1 thermal activated channel by activating TRPV2 to generate analgesic activity for preparing an analgesic drug. The centipede analgesic peptide SLP_RhTx has the advantages that the analgesic peptide can activate and inactivate the TRPV1 channel and specifically inactivate the TRPV2 thermal activated channel so as to generate a powerful analgesic function, and meanwhile, balance of a body temperature adjusting system is not affected. In addition, the analgesic peptide has the characteristics of being simple in structure, convenient to artificially synthesize, quick to respond, strong in analgesic activity and the like, is an excellent candidate medical molecule which takes TRPV1 as an analgesic drug target, and has an important prospect for developing and applying analgesic drugs.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to centipede analgesic polypeptide SLP_RhTx and its gene, cDNA and application. Background technique: [0002] Centipede is a kind of traditional Chinese medicine in my country. It has the effects of detoxifying and anti-cancer, calming wind and relieving spasm. Less spiny centipede (S. subspinipesmutilans L. Koch) is a similar species of mold spinipes. Mainly distributed in China and Japan. Common in the middle and lower reaches of the Yangtze River. It hides under rocks or among rocks during the day, and hunts insects (such as crickets, grasshoppers, scarabs, and various moths) at night, and can also kill small vertebrates (such as sparrows, lizards, snakes, etc.) with its poisonous jaws. ). In humans, a centipede's bite can be excruciatingly painful, similar to a high-temperature burn. Such physiological phenomenon may be caused by the activation of TRPV1 channel. [0003] TRPV1 is...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/20C07K1/16C12N15/12A61K38/17A61P29/00G01N24/08
CPCC07K14/43513A61K38/00G01N24/087
Inventor 赖仞杨仕隆
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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