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Microspheres with high fluorescence intensity and preparation method for microspheres

A technology of fluorescence intensity and microspheres, which is applied in the preparation of microspheres and high fluorescence intensity microspheres, can solve the problems of low quantum yield, complicated preparation process, difficulty in obtaining quantum dot fluorescent microspheres, etc., and achieve fluorescence intensity Improved effect

Active Publication Date: 2015-11-25
HANGZHOU JOINSTAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with oil-soluble quantum dots, water-soluble quantum dots have a lower quantum yield, and the preparation process of the layer-by-layer self-assembly method is complicated, and it is difficult to obtain quantum dot fluorescent microspheres with high fluorescence intensity.

Method used

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  • Microspheres with high fluorescence intensity and preparation method for microspheres
  • Microspheres with high fluorescence intensity and preparation method for microspheres
  • Microspheres with high fluorescence intensity and preparation method for microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Step 1. Weigh 1 portion of 6.85 mg polystyrene mesoporous microspheres with a pore size of 30 nm on a precision balance, and add it to a 2 mL centrifuge tube;

[0045] Step 2. Weigh 40 mg polystearyl maleic anhydride (PMAO, Mn ~ 30000-50000), dissolve it in 1 mL chloroform, and set aside;

[0046] Step 3. Preparation of alkaline aqueous solution: absorb 200 μL of 1M sodium hydroxide aqueous solution, add it to 4 mL of ultrapure water, and store in a 10 mL glass bottle;

[0047] Step 4, take 120 μL of LCdSe / ZnS dispersion (n-hexane dispersion, 30 μM) in a 500 μL centrifuge tube, add 200 μL of ethanol for flocculation;

[0048] Step 5, centrifuging the quantum dot ethanol mixture in step 4 (3min, 8000rpm), pouring the supernatant after centrifugation;

[0049] Step 6. Volatilize the residual ethanol on the surface of the quantum dot particles obtained in step 5 at room temperature, and then redisperse the quantum dots in 100 μL of chloroform;

[0050] Step 7. Add 50 μL ...

Embodiment 2

[0058] Step 1. Weigh 1 portion of 6.85 mg polystyrene mesoporous microspheres with a pore size of 30 nm on a precision balance, and pour it into a 2 mL centrifuge tube;

[0059] Step 2. Weigh 40 mg of poly(styrene-co-maleic anhydride) (PSMA, Mn~1700), dissolve it in 1 mL of chloroform, and set aside;

[0060] Step 3. Preparation of alkaline aqueous solution: absorb 200 μL of 1M sodium hydroxide aqueous solution, add it to 4 mL of ultrapure water, and store in a 10 mL glass bottle;

[0061] Step 4, take 120 μL of LCdSe / ZnS dispersion (n-hexane dispersion, 30 μM) in a 500 μL centrifuge tube, add 200 μL of ethanol for flocculation;

[0062] Step 5, centrifuging the quantum dot ethanol mixture in step 4 (3min, 8000rpm), pouring the supernatant after centrifugation;

[0063] Step 6. Volatilize the residual ethanol on the surface of the quantum dot particles obtained in step 5 at room temperature, and then redisperse the quantum dots in 100 μL of chloroform;

[0064] Step 7. Add 5...

Embodiment 3

[0072] Step 1. Weigh 1 part of 1.37 mg polystyrene mesoporous microspheres with a pore size of 30 nm on a precision balance, and add it to a 2 mL centrifuge tube;

[0073] Step 2, take 24μL of LCdSe / ZnS dispersion (n-hexane, 30μM) in a 500μL centrifuge tube, add 50uL of ethanol for flocculation;

[0074] Step 3, centrifuging the quantum dot ethanol mixture in step 2 (3min, 8000rpm), pouring the supernatant after centrifugation;

[0075] Step 4. Volatilize the residual ethanol on the surface of the quantum dot particles obtained in step 3 at room temperature, and then redisperse the quantum dots in 30 μL of chloroform;

[0076] Step 5. Add 0.4 mg of polystearyl maleic anhydride to the quantum dot chloroform dispersion obtained in step 4, and ultrasonically disperse to make the mixture uniform;

[0077] Step 6. Add 30 μL of the quantum dot chloroform dispersion obtained in step 5 to the weighed 1.37 mg polystyrene microspheres, so that the polystyrene particles are completely i...

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Abstract

The invention discloses microspheres with high fluorescence intensity and a preparation method for the microspheres. The preparation method comprises: dispersing oil soluble quantum dots and macromolecular mesoporous microspheres in chloroform; and by taking a maleic anhydride copolymer as a surface activity mediating molecule, adding an alkaline aqueous solution or an amine compound, wherein the oil soluble quantum dots enter into ducts of the macromolecular mesoporous microspheres so as to obtain the fluorescent microspheres with abundant carboxyl functional groups on the surface. Compared with a conventional swelling method, according to the fluorescent microspheres prepared by the preparation method, the fluorescence intensity is improved by over 10 times, and the fluorescent microspheres have abundant functional groups on the surface, so that the fluorescent microspheres are favorably applied to the downstream biomedical field.

Description

technical field [0001] The invention relates to a method for preparing microspheres, in particular to a method for preparing microspheres with high fluorescence intensity, and belongs to the field of nanobiological materials. Background technique [0002] Suspension microarray technology based on fluorescently encoded microspheres has the ability to simultaneously screen and quantify multiple proteins and cytokines in the same sample, and has high research and application value in the field of disease diagnosis. The core technology of suspension microarray technology is coded microspheres with unique labeling signals during the detection process, which can be obtained by marking organic fluorescent groups, Raman probes, or quantum dot nanocrystals and other luminescent materials. Compared with other luminescent materials, quantum dots are considered as ideal fluorescent coding materials because of their advantages such as wide excitation spectrum, narrow emission spectrum, a...

Claims

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Application Information

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IPC IPC(8): B01J13/14C09K11/88C09K11/02
Inventor 古宏晨徐宏张鼎晟子王叶菲
Owner HANGZHOU JOINSTAR BIOTECH
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