Frozen semen artificial insemination method for increasing conception rate of donkeys

Abstract

The invention discloses a frozen semen artificial insemination method for increasing the conception rate of donkeys. The method includes the steps of fresh semen freezing treatment, frozen semen unfreezing treatment and ovulation induction timing insemination. In the process of implementation, first, collected semen of the male donkeys is diluted and placed into a full-automatic freezing instrument to automatically complete freezing, wherein the motility rate is larger than or equal to 70%; before artificial insemination is performed on the female donkeys to be pregnant, the semen is rapidly placed into a water bath of 38 DEG C to be instantly unfrozen, and the step of ovulation induction timing insemination is completed within 1 hour. By the adoption of the method, the technical measure of ovulation induction timing insemination is adopted, so that the conception rate at estrus reaches 70%.

Description

technical field [0001] The invention relates to a method for artificial insemination of livestock. technical background [0002] Donkeys belong to the genus Equus. In order to increase the breeding capacity, artificial insemination is generally adopted. Due to the particularity of the ovarian structure of equine animals, the duration of estrus changes greatly after the appearance of estrus, which generally lasts for 3 to 14 days. There is a big difference, the ovulation time is mostly on the 7th to 10th day of estrus, and the ovulation varies from 12 to 24 hours before the cessation of estrus, which makes it difficult to determine the time of donkey insemination. The traditional technique is through rectal palpation of the ovaries, and insemination is performed when the follicles are developed until the follicles are soft, elastic and fluctuating, that is, the third and fourth stages of rectal examination, but the rectal palpation technique requires technicians A long time...

AI Technical Summary

Problems solved by technology

Due to the particularity of the ovarian structure of equine animals, the duration of estrus changes greatly after the appearance of estrus, which generally lasts for 3 to 14 days. There is a large difference, the ovulation time is mostly on the 7th to 10th day of estrus, and the ovulation varies from 12 to 24 hours before the cessation of estrus, which makes it difficult to determine the time of donkey insemination

The traditional technique is through rectal palpation of the ovaries, and insemination is performed when the follicles are developed until the follicles are soft, elastic and fluctuating, that is, the third and fourth stages of rectal examination, but the rectal palpation technique requires technicians A long time of experience has accumulated, and different people have different judgments on the stage of follicle development. Even if the stage of follicle development is accurately determined, it is difficult to accurately determine the time of ovulation. Follicles at stage 3 will last for 1 to 3 days, and follicles at stage 4 will last for 1 to 3 days. It will also last for 1-3 days, and it is difficult to determine the ovulation time, which makes it difficult to control the ovulation during estrus. In addition, the frozen semen of the donkey is not handled properly, the survival rate of the frozen semen is low, and the conception rate after one artificial infusion of frozen semen Less than 25%, multiple artificial insemination of donkeys is required to increase the conception rate, which is time-consuming and labor-intensive