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Composite microspheres for ordered release of growth factors and antibiotics, preparation method and application

A growth factor and composite microsphere technology, applied in the field of biomedical materials, can solve the problems of a large amount of burst release, the generation of acidic environment by microsphere degradation products, and the limitation of PLGA microspheres, and achieve good antibacterial, anti-inflammatory, and good biodegradability. , the effect of reducing the bursting phenomenon

Inactive Publication Date: 2018-01-12
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, PLGA microspheres have a very broad application prospect as a sustained-release drug delivery system, but there are also some disadvantages, such as a large amount of sudden drug release in the initial stage of sustained release, and acidic environment caused by degradation products of microspheres, etc., which limit PLGA microspheres. Practical application of the ball

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  • Composite microspheres for ordered release of growth factors and antibiotics, preparation method and application
  • Composite microspheres for ordered release of growth factors and antibiotics, preparation method and application

Examples

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Effect test

Embodiment 1

[0022] Example 1: A preparation method for ordered release of growth factor and antibiotic composite microspheres

[0023] 1) Dissolve 80 mg of PLGA polymer in 2 mL of dichloromethane to obtain an oil phase; dissolve 2 μg of bFGF in 200 μL of deionized water and slowly add it to the oil phase, and emulsify at 20,000 rpm / min for 1 min with a high-speed homogenizer Get colostrum W1 / O;

[0024] 2) Dissolve 250 mg PVA in 25 mL deionized water to obtain a 1% PVA aqueous solution; dissolve 50 mg GC and 125 mg chlorhexidine acetate (CHA) in the above PVA aqueous solution to obtain a GC / CHA / PVA solution;

[0025] 3) Slowly add the colostrum W1 / O obtained in step 1) to the GC / CHA / PVA solution obtained in step 2), and use a high-speed homogenizer to stir at 12000 rpm / min for 3 minutes to obtain double milk W1 / O / W2;

[0026] 4) The double emulsion was magnetically stirred at 800 r / min for 4 hours, centrifuged and washed with deionized water for 5 times, and freeze-dried to obtain comp...

Embodiment 2

[0027] Example 2: A preparation method for ordered release of growth factor and antibiotic composite microspheres

[0028] 1) Dissolve 100 mg of PLGA polymer in 2 mL of dichloromethane to obtain an oil phase; dissolve 2 μg of bFGF in 200 μL of deionized water and slowly add it to the oil phase, and emulsify at 15,000 rpm / min with a high-speed homogenizer1 min to get colostrum W1 / O;

[0029] 2) Add 500 mg PVA to 25 mL deionized water to obtain a 2% PVA aqueous solution; dissolve 125 mg GC and 25 mg CHA in the above PVA aqueous solution to obtain a GC / CHA / PVA solution;

[0030] 3) Slowly add the colostrum W1 / O obtained in step 1) to the GC / CHA / PVA solution obtained in step 2), and use a high-speed homogenizer to stir at 8000 rpm / min for 3 minutes to obtain double milk W1 / O / W2 ;

[0031] 4) The double emulsion was magnetically stirred at 800 r / min for 4 hours, centrifuged and washed with deionized water for 5 times, and freeze-dried to obtain composite microspheres co-loaded wi...

Embodiment 3

[0032] Example 3: In vitro release experiment of ordered release growth factor and antibiotic composite microspheres

[0033] 1) Weigh 10 mg of composite microspheres and put them in 1 mL of PBS solution (pH 7.4), shake at 37°C and 110 rpm, use BCA kit to measure the release of growth factors and calculate their cumulative release ; Using a UV-Vis spectrophotometer at 253 nm to measure the release of chlorhexidine acetate and calculate its cumulative release rate. Three parallel samples were performed, and the results were expressed as mean ± standard deviation.

[0034] 2) Experimental results: by figure 2 It can be seen that the release rate of chlorhexidine acetate is fast, with a cumulative release of more than 90% within one week, and the release of bFGF is slow, indicating that the composite microspheres can release growth factors and antibiotics in an orderly manner, so as to quickly inhibit inflammation and continuously induce disease-deficient tissue. purpose of re...

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Abstract

The invention relates to a compound microsphere for orderly releasing growth factors and antibiotics and its preparation method and application. The PLGA microspheres co-loaded with growth factors and antibiotics are modified by ethylene glycol chitosan, wherein the antibiotics are encapsulated in ethylene glycol. The chitosan shell contains growth factors in the PLGA core; the composite microspheres have an orderly release function, and the antibiotics in the shell are released quickly to achieve rapid anti-inflammatory effects, while the growth factors in the core are slowly released to induce tissue regeneration. The composite microsphere prepared by the invention has high drug encapsulation rate, simple preparation process and easy industrialization; it can be applied to the preparation of carrier materials for repairing diseased and defective periodontal tissue, bone or cartilage tissue.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a composite microsphere orderly releasing growth factors and antibiotics, a preparation method and application thereof. Background technique [0002] Soft and hard tissue disease defects are usually accompanied by infection. To realize the regeneration and repair of tissue disease defects, the inflammation must be eliminated first. Therefore, there is an urgent need to invent a drug carrier that can realize the orderly release of growth factors and antibiotics in the clinic, and rapidly inhibit inflammation while continuously inducing the repair of diseased and defective tissues. At present, polymer drug-loaded microspheres have attracted widespread attention, because they have the function of sustained release of drugs and targeted drug delivery, which can improve the bioavailability of drugs; in addition, microspheres can be administered through subcuta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K47/36A61K47/34A61K38/18A61K45/00A61P29/00A61P31/04A61L27/48A61L27/54A61L27/50
Inventor 张其清陈名懋曹欢刘园园方哲翔
Owner FUZHOU UNIV
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