Directional mating method for Penaeus vannamei Boone

Abstract

The invention discloses a directional mating method for Penaeus vannamei Boone. The method includes the steps that parent prawns are selected and subjected to nutrient enrichment cultivation, a mating scheme is set, male prawns are ripened and independently cultivated, female prawns are ripened and independently cultivated, directional mating parent prawns are selected and subjected to directional mating, and spawning and fertilization are performed. The method is designed according to the life habits and the mating, fertilization and spawning characteristics of the Penaeus vannamei Boone, and possible injuries to the male prawns and the female prawns in the artificial fertilization process can be avoided. In the whole mating process, it is only needed to determine the serial numbers of the parent prawns for mating, operation is convenient and easy, and the genealogical relationship of established families can be accurately established. The genetic relationship of the individuals of the families is determined, the mating scheme is designed artificially, and inbreeding depression of the established families can be effectively avoided. The method has the advantages of being high in success rate and low in parent prawn loss, and overcomes the defects that in the prior art, when Penaeus vannamei Boone families are established in an artificial fertilization mode, spermatophore is prone to falling off, the fertilization rate is low, and half sibs cannot be established easily.

Description

technical field [0001] The invention relates to a family selective breeding of Penaeus vannamei, in particular to a method for constructing a family of Penaeus vannamei for realizing directional mating of prawns according to individual markers. Background technique [0002] Litopenaeus vannamei ( Litopenaeus vannamei ), also known as Penaeus vannamei, belongs to the phylum Arthropoda (Athropoda), crustacean (Crustacean), decapoda (Decapoda), and is naturally distributed along the Pacific coast from Peru to Mexico. Litopenaeus vannamei has the characteristics of delicious meat, strong stress resistance, fast growth, long breeding period, high density and low salinity resistance, and is conducive to the transportation of live shrimp. After Litopenaeus vannamei was introduced into my country in the 1990s, it quickly solved the key technology of large-scale seed breeding, formed aquaculture models and technologies for different regional aquaculture environments in my country, p...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for constructing the Penaeus vannamei family by realizing directional mating of prawns instead of artificial fertilization according to individual marks. The artificial insemination method has problems such as damage to broodstock, easy shedding of sperm pods, low fertilization rate, and difficulty in establishing half-siblings.