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Screening method for virus-free potato test-tube plantlet early blight prevention

A screening method and potato technology, which is applied in the field of screening anti-early blight materials, can solve the problems that are not suitable for the evaluation of anti-early blight of virus-free potato plantlets in test tubes, have large deviations, and take a long time to detect, so as to achieve small errors and improve timeliness , the effect of screening low cost

Active Publication Date: 2015-11-11
XINJIANG YUWEIXIAN AGRI SCI & TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a screening method for anti-early blight of virus-free potato plantlets, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the existing early blight The evaluation method has the problems of large deviation, long detection time and high cost, and is not suitable for the evaluation of virus-free potato plantlets against early blight

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1, the screening method of the anti -villain potato test tube seedlings resisting early epidemic, according to the following steps: the first step, the separation of pathogenic bacteria, collect potato premature disease leaf in different regions, collect potato premature epidemic leaf leavesRinse through the tap water once to 3 times, soak 20s to 30s in the ethanol water solution, soak it in the mercury solution after soaking for 10s to 20s, rinse with sterile water 3 times to 5 times, and then use it to destroy it.Take out the leaves of the diseased and healthy junction of the bacterial anatomical knife, place the back of the leaves of the sickness at the back of the PDA tablet, put 3 to 4 leaves per dish, and place it in the dark cultivation of dark culture in the training box of 25 ° C to 3D to 3D to5D, separate the pathogens on the leaves after cultivation to obtain pathogenic bacteria;

[0016] In the second step, the purification of pathogen bacteria, placed t...

Embodiment 2

[0021] Example 2, as the optimization of the above embodiments, the PDA medium is obtained according to the method as follows: the 200g fresh potato, washed and peeled, add ionic water 1000ml to boil for 15min, add ionic water to 1000ml after filtering gauze filtering to 1000mlAdd 12g of glucose and 10g to 12g agar powder. After boiling the agar powder completely melted, the PDA medium is obtained.After boiling, the agar powder is completely melted; the packed PDA medium should be sterilized within 1H, and the high -pressure steam is sterilized. The temperature is 121 ° C under the 0.1MPa steam, and the temperature is 121 ° C. It is maintained for 20min.

[0022] Dacking method: A.Triangular bottle (cone bottle) is packed; 250ml of triangular bottle is packed. Each bottle is filled with about 200ml medium.

[0023] B.IVF; test tubes to make a slope medium, which are mainly used for the transfer and preservation of bacteria.Take a clean test tube of 20ml, add about 5ml (1 / 4 test tu...

Embodiment 3

[0026] Example 3, as the optimization of the above embodiments, the size of the leaves at the junction of the disease and health is 0.5cm 2 To 1cm 2 Essence

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Abstract

The invention relates to the technical field of early blight prevention material screening methods, and discloses a screening method for virus-free potato test-tube plantlet early blight prevention. The method includes the following steps that firstly, pathogenic bacteria are separated; secondly, the pathogenic bacteria are purified; thirdly, sporangium turbid liquid is prepared; fourthly, inoculation is carried out. Compared with a traditional screening method, the screening method for virus-free potato test-tube plantlet early blight prevention is small in error, short in screening time and low in screening cost. Meanwhile, the screening method for virus-free potato test-tube plantlet early blight prevention makes up for the blanks in the aspect of potato early blight research and prevention in the Xinjiang region. The screening method suitable for potato early-blight-prevention test-tube plantlets in the Xinjiang region is established, timeliness, effectiveness and accuracy of early blight identification are greatly improved, and the method plays has great significance in quick epidemic disease identification and prevention.

Description

Technical field [0001] The invention involves the technical field of anti -premature epidemic materials screening method. It is a screening method for anti -early epidemic disease to resist early -dotted potato test tube seedlings. Background technique [0002] Potato is a one -year mycotus plant in the genus Egishin family. It is an important crop for food and vegetables.Potato premature disease caused by the infection of eggplant groma is severe diseases in potato production, which occurs in all potato production areas, and aggravate the trend year by year.Especially in developing countries, it is considered to be the second largest potato disease that is second only to potato disease.In terms of production, there are many evaluations of potato disease and viral diseases, and less research on premature diseases.And the existing early epidemic evaluation method has large deviations, long testing time and high cost, and is not suitable for the evaluation of anti -virus potato tes...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨茹薇冯怀章罗正乾孙慧徐琳黎吴燕古力米拉·热合木土拉朱荣贵倪萌李江涛沈洪飞马静
Owner XINJIANG YUWEIXIAN AGRI SCI & TECH CENT
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