Method for identifying and evaluating cold resistance of ramie germplasm resources
A technology of cold resistance and ramie, which is applied in the field of cold resistance identification and evaluation of ramie germplasm resources, can solve problems such as difficulty in unifying freezing damage temperature, no feasible cold resistance identification method, inconvenient judgment, etc., and achieves stable and reliable evaluation and classification data, It is convenient for cold resistance identification operation and statistical effect
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Embodiment 1
[0025] Use the method provided by the invention to evaluate the cold resistance of ramie germplasm resources
[0026] (1) Ramie germplasm seedling cultivation:
[0027] The 15 ramie germplasms used in this experiment were provided by the National Germplasm Ramie Garden of the Institute of Hemp, Chinese Academy of Agricultural Sciences, namely: Hubei fine leaf green, Wanxian ramie, green ramie, Japanese ramie, Yihuang ramie, Sichuan ramie No. 2, Dayong yellow shell hemp, Huangpi ramie, Zhongzhu No. 1, Qianzhu No. 1, Jiujiang hemp, Liping green hemp, Chuanzhu No. 8, Longdijia green hemp, and Qingpi ramie. On March 10, 2014, the ramie seed bushes were excavated from the ramie germplasm garden, and the seed bushes were required to be free of viruses and rot. Finely cut ramie seed bushes, plant about 25g seed bushes with 8-10 bud points in plastic flower pots, and repeat 3 times in 5 pots for each germplasm.
[0028] The soil configuration method in the flower pot is: the loess i...
Embodiment 2
[0037] Embodiment 2 comparative example
[0038] Correlation between frostbitten leaves and plant mortality of ramie germplasm under low temperature stress
[0039] The most intuitive effect of low temperature stress is the change of plant phenotype, especially the change of morphological characteristics. Plant death is one of the changes in morphological characteristics, but the first sensitive response to low temperature stress is the leaves, so some scientists evaluate the cold resistance of plant germplasm by leaf browning and shedding. The leaves of ramie began to brown at the edges under 4℃ stress for 2 days, and the browning degree of leaves increased under 2℃ stress, and the leaves appeared to fall off after 2 days of stress. In this case, the correlation between frostbitten leaves of ramie germplasm under 2°C low temperature stress and plant mortality of 0°C stress for 2 days was studied (see Table 2). Although there is a big difference between browned leaves and fa...
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