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A kind of multi-layer semi-dry adherent culture device and culture method

A technology of adherent culture and culture method, which is applied in the field of multi-layer semi-dry adherent culture devices, can solve the problems of uneven distribution of light intensity, and achieve the effects of uniform illumination, reduced light energy loss, and consistent growth status

Inactive Publication Date: 2017-04-19
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a multi-layer semi-dry adherent culture device, which not only solves the problem of uneven distribution of light intensity, but also facilitates inoculation and harvesting, and realizes light energy. Efficient use of

Method used

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  • A kind of multi-layer semi-dry adherent culture device and culture method
  • A kind of multi-layer semi-dry adherent culture device and culture method
  • A kind of multi-layer semi-dry adherent culture device and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Place six adherent culture units 2 horizontally on the support system 1. The adherent culture units 2 are 1m wide and 5cm high, and the lengths from top to bottom are 1m, 1.2m, 1.4m, 1.6m, 1.8m, 2.0 m. All adherent culture units 2 are aligned near the side of the algae mud scraper 7, and the other side is distributed in steps, and the next layer is 0.2m wider than the upper layer, so that each layer of adherent culture units 2 has a part that can accept vertical The light on the ground is not blocked by other layers, the interlayer height of the adherent culture unit 2 is 0.1m, and the moving surface of the adherent culture unit 2 is a stainless steel wire mesh conveyor belt, which is driven by the same motor 12 to rotate . Ordinary towels are spread and fixed on steel wire mesh as the culture surface. The Spirulina medium used in this embodiment consists of 1 liter of medium containing: 16.80 grams of sodium bicarbonate, 0.5 grams of potassium hydrogen phosphate, 2.5 ...

Embodiment 2

[0042] Place 3 adherent culture units 2 on the support system 1, each adherent culture unit 2 is composed of a fiber fabric belt group to form a closed conveyor belt with a width of 1m and a height of 5cm, and the lengths of the adherent culture units 2 from top to bottom are 1.0 m, 1.5m, 2.0m. All adherent culture units 2 are fixed on the support system 1 at an angle of 30 degrees with the horizontal, such as Figure 4 As shown, two adherent culture units are placed in parallel and obliquely, and a part of any layer directly receives the light perpendicular to the ground without being blocked by other layers. The distance between adjacent adherent culture units 2 is 0.1 m. One end of each adherent culture unit 2 is fixed with a drive sprocket, and multiple adherent culture units 2 are coupled with the drive chain 9 through the drive sprocket and rotate under the same motor 12 . The culture device is placed outdoors to receive natural light, and the light intensity of sunlig...

Embodiment 3

[0044] The culture device is the same as in Example 2, inoculate the seed liquid containing 900g (dry weight) spirulina on the same culture area, open the medium delivery pump 11 and adjust the flow to make the culture liquid drip onto the adherent culture unit 2 to keep the culture surface moist . After cultivating for 3 days, all the biomass of the spirulina was collected to obtain a dry weight of 1342 grams. After deducting the inoculated biomass of 900 grams, the device covers an area (based on the area of ​​the largest adherent culture unit 2) of 1m×2m=2m 2 , equivalent to (1342-900) g / 2m yield per unit area of ​​Spirulina 2 / 3d=73.7g / m 2 / d.

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Abstract

The invention belongs to the field of microalgae culture and specifically relates to a multilayer semi-dry adherent culture device provided with multilayer adherent culture units. The adherent culture units are arranged horizontally or arranged forming a certain inclination angle from the horizontal plane, and one end of each multilayer adherent culture unit is aligned and the multilayer adherent culture units are parallelly arranged on a bracket system. Lengths of the multilayer adherent culture units are progressively increased successively from top to bottom, and the multilayer adherent culture units are driven by a motor to rotate. During operation of the device, a microalgae seed solution is firstly sprayed through a microalgae inoculators onto each layer of the adherent culture unit successively; a medium drips onto culture surfaces of all the adherent culture units through a medium distributor, and the surplus liquid returns back to a medium collecting tank under the action of gravity so as to be repeatedly used; and when an algae mud scraper is used for harvesting algae mud, the blade sticks close to the surfaces of the adherent culture units, and algae mud is scrapped off into an algae mud collecting tank with rotating of the adherent culture units. According to the invention, the problem of uneven light distribution is solved, the device is convenient for inoculation and harvesting, and efficient utilization of luminous energy is realized.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, in particular to a multi-layer semi-dry adherent culture device provided with multi-layer adherent culture units. Background technique [0002] Microalgae refers to aquatic planktonic algae capable of photosynthesis. Some microalgae are rich in protein and can be used as aquatic bait or livestock feed (such as spirulina). More importantly, some microalgae can synthesize a large amount of secondary metabolites under specific conditions, such as oils, carotenoids, polysaccharides, etc. These substances are often biologically active substances with high economic value and can be used in functional foods, Food additives, pharmaceuticals, bioenergy and other fields. In particular, extracting microalgae oil through large-scale cultivation of microalgae, and then transforming it into biodiesel is considered to be one of the most important ways to solve the problem of bioenergy production and carb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N1/12C12R1/89
Inventor 刘天中王俊峰陈林汪辉高莉丽周文俊张维董庆喆
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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