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Preparation method for desert soil micro drip irrigation microbial bacterial manure

A microbial fertilizer and soil technology, applied in the direction of fertilization device, fertilizer mixture, application, etc., to increase effectiveness, prevent salinization, solve the effect of nutrient supply and soil quality improvement

Inactive Publication Date: 2015-10-28
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a kind of micro-drip irrigation microbial bacterial fertilizer for desert soil aiming at the problems existing in desert soil barren treatment at present, especially a kind of special microbial bacterial fertilizer preparation method which is comprehensive in nutrition and suitable for drip irrigation under the condition of drip irrigation in desert saline-alkali areas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis subsp.subtilis), Potsdam bacillus (Brevibacillus borstelensis), colloid bacillus (Bacillus mucilaginosus), Rhodopseudomonas palustris (Rhodop seudanonas palustris), 6 kinds of fermentation stocks of Azotobacter vinelandii Lipman were mixed according to volume ratio 1:1:1:2:2; 3 ratio, in (NH 4 ) SO 4 2.0g / L, NaH 2 PO 4 1.5g / L, pH6.5, 2.0% starch aqueous solution by mass, aeration and stirring fermentation under the condition of 34℃, 48h, until the microbial content in the culture solution is not less than 4.0×10 8 CFU (colony forming unit) / mL, that is, to obtain the desert soil micro-drip irrigation biological liquid bacterial fertilizer, or to obtain the desert soil micro-drip irrigation biological powder bacterial fertilizer by spray drying.

Embodiment 2

[0025] Embodiment 2: Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis subsp.subtilis (Bacillus subtilis subsp.subtilis), Bacillus Potsdam (Brevibacillus borstelensis), Bacillus mucilaginosus (Bacillus mucilaginosus), Rhodopseudomonas palustris (Rhodop seudanonas palustris), 6 kinds of fermentation stocks of Azotobacter vinelandii Lipman were mixed according to volume ratio 1:1:1:2:2; 3 ratio, in (NH 4 ) SO 4 4.0g / L, NaH 2 PO 4 3.0g / L, pH7.5, 2.0% by mass starch aqueous solution, under the conditions of 28°C, 36h, aeration and stirring fermentation, until the microbial content in the culture solution is not less than 4.0×10 8 CFU (colony forming unit) / mL, that is, to obtain the desert soil micro-drip irrigation biological liquid bacterial fertilizer, or to obtain the desert soil micro-drip irrigation biological powder bacterial fertilizer by spray drying.

Embodiment 3

[0026] Embodiment 3: Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis subsp.subtilis), Potsdam bacillus (Brevibacillus borstelensis), colloid bacillus (Bacillus mucilaginosus), Rhodopseudomonas palustris (Rhodop seudanonas palustris) ), six kinds of fermentation stocks of Azotobacter vinelandii Lipman were mixed according to the ratio of volume ratio 1:1:1:2:2; 3, in (NH 4 ) SO 4 3.0g / L, NaH 2 PO 4 2.5g / L, pH7.0, 2.0% starch aqueous solution by mass, aeration and stirring fermentation under the condition of 30℃, 42h, until the microbial content in the culture solution is not less than 4.0×10 8 CFU (colony forming unit) / mL, that is, to obtain the desert soil micro-drip irrigation biological liquid bacterial fertilizer, or to obtain the desert soil micro-drip irrigation biological powder bacterial fertilizer by spray drying.

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Abstract

The invention discloses a preparation method for desert soil micro drip irrigation microbial bacterial manure. The method includes the steps of conducting fermentation in a basic culture medium of one of similar bacterial strains of six types of bacteria including bacillus licheniformis, bacillus subtilis, Potsdam bacillus, bacillus mucilaginosus, rhodopseudomonas palustris and azotobacter vinelandii according to the inoculation quantity of 2.0% at the temperature of 28 DEG C to 37 DEG C and at the speed of 180 r / min for 36 hours to obtain original fermentation fluid of all the bacteria, mixing the six types of original fermentation fluid according to the size ratio of 1 to 1 to 1 to 2 to 2 to 3, and conducting ventilating, stirring and fermenting in a starch aqueous solution for 36 hours to 48 hours at the temperature of 28 DEG C to 34 DEG C till the microorganism content in the culture solution is higher than or equal to 4.0 *108 colony-forming unit (CFU) / mL to obtain the desert soil micro drip irrigation microbial bacterial manure, wherein the starch aqueous solution contains (NH4)SO4 with the density of 2.0 g / L to 4.0 g / L and NaH2PO4 with the density of 1.5 g / L to 3.0 g / L, the pH of the starch aqueous solution ranges from 6.5 to 7.5, and the percentage mass content is 2.0%.

Description

technical field [0001] The invention relates to microbial bacterial fertilizer, in particular to a preparation method for micro-drip irrigation microbial bacterial fertilizer in desert soil. Background technique [0002] Microbial fertilizer, referred to as bacterial fertilizer, also known as microbial inoculant. It is fermented (artificially cultivated) by microorganisms with special effects, and contains a large number of beneficial microorganisms. After being applied to the soil, it can fix nitrogen in the air, or activate nutrients in the soil, and improve plant nutrition. The environment, or the specific microbial products that produce active substances and stimulate plant growth during the life activities of microorganisms, play an important role in the improvement of soil quality, high crop yield, high-quality and efficient agriculture. [0003] Micro-drip irrigation technology is an irrigation method with better water-saving effect in the world today. The principle...

Claims

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Application Information

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IPC IPC(8): C05G3/04C05G3/80
Inventor 张宇曾雪
Owner SOUTHWEAT UNIV OF SCI & TECH
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