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Maize yellow-green leaf gene ygl-1, and coded protein and application thereof

A technology of corn yellow-green leaves and mutation genes, applied in the field of genetics, can solve the problem of preliminary positioning of limited genes

Active Publication Date: 2015-10-14
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some leaf color mutants have been obtained in maize, but most of the research is limited to the preliminary mapping of genes

Method used

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  • Maize yellow-green leaf gene ygl-1, and coded protein and application thereof
  • Maize yellow-green leaf gene ygl-1, and coded protein and application thereof
  • Maize yellow-green leaf gene ygl-1, and coded protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Phenotype and Genetic Analysis

[0030] (1) Analysis of blade characteristics

[0031] The maize ygl-1 mutant was bred inbred lines (two maize inbred lines are Yuanwu 02 and Ye 478, the materials are common breeding materials in this unit, Dr. found in the progeny materials found in the test base), the leaves of the whole growth period are light green ( figure 2 ).

[0032] (2) Determination of chlorophyll-related components

[0033] At the seedling stage and the adult plant stage, samples were taken from the corresponding positions of the leaves of the normal plant Lx7226 and the yellow leaf mutant ygl-1, and the contents of chlorophyll a, chlorophyll b and carotenoids were measured. Compared with normal plants, the contents of chlorophyll a, chlorophyll b and carotenoids in yellow-leaf mutants in the early stage decreased significantly, and the content of chlorophyll b decreased the most. It is speculated that the phenotype of yellow-green leaf mutant yg...

Embodiment 2

[0044] Example 2 Location of mutant gene ygl-1 site

[0045]Using 224 pairs of core SSR primers synthesized in the laboratory, a polymorphic molecular marker P3 linked to the target gene was found by means of BSA segregation analysis method, located in the Bin1.01 region, and the known functional gene of the yellow-green leaf mutant in maize elm1(8.06), elm2(9.03) and vyl(Chr.9) are not on the same chromosome. It is preliminarily concluded that the gene controlling this trait is a new gene whose function is still unclear. Using the 23 pairs of SSR primers in the Bin1.01 area published on the maizegdb website to mine polymorphic molecular markers linked to the target gene, 5 polymorphic markers were found, namely P1, P2, P4, P5, and P6 (as shown in Table 3). Show). These six polymorphic molecular markers were used to detect the genotypes of 231 yellow-green leaf mutants in the F2 segregation population, and combined with their phenotypes, a genetic linkage map of the yellow-gr...

Embodiment 3

[0051] Example 3 Cloning of mutant gene ygl-1

[0052] The sequence of the gene coding region was amplified from wild-type and mutant leaf genomes using PCR primers (as shown in SEQ ID NO.3 and 4), directly connected to the cloning vector, sequenced, and then compared with the sequence analysis software DNAStar. PCR amplification program: 95°C for 5min, 95°C for 45s, 65°C for 45s, 72°C for 90s, a total of 30 cycles, finally 72°C for 10min, 4°C forever.

[0053] Sequence analysis found that the mutant gene ygl-1 lacked a G at ORF713, which caused a frameshift mutation in the coding region and caused premature termination ( Image 6 ).

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Abstract

The invention discloses a maize yellow-green leaf gene ygl-1 disclosed as SEQ ID NO.1 and a coded protein thereof disclosed as SEQ ID NO.2. The invention also discloses primers for amplifying the maize yellow-green leaf gene ygl-1, which are disclosed as SEQ ID NO.4 and SEQ ID NO.5. The maize yellow-green leaf gene ygl-1 is detected in maize for the first time, can be used for maize high-quality seed breeding and crossbreeding, and can be used as a molecular marker for germplasm resource identification. After the germplasm material or transformed descendant containing the maize yellow-green leaf gene ygl-1 is selected, the marker can be utilized to accurately select the genotype of the backcross transformed descendant, and can also be used for screening corn germplasm resources to search for the mutant material with more abundant genetic background, thereby having important application value for improving the hybrid seed purity control effect.

Description

technical field [0001] The invention belongs to the field of genetics, and in particular relates to a corn yellow-green leaf mutation gene ygl-1, a protein encoded by the gene and an application thereof. Background technique [0002] Among all the mutation types existing in nature, the leaf color mutation is one of the most common mutation forms, and the leaf color mutation can usually be displayed intuitively, the characters are relatively obvious, and it is easy to identify and distinguish. As early as the 1930s, there were reports of related leaf color mutants in biological research. So far, leaf color mutants have been found in various plants such as rice, soybean, corn, barley, wheat, tomato and rapeseed. The mutated genes often directly or indirectly affect the synthesis and degradation of photosynthetic pigments, especially chlorophyll, resulting in changes in the content and proportion of various pigments, thereby causing variations in plant leaf color. [0003] Ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/68C12N15/11
Inventor 关海英汪黎明何春梅徐相波刘铁山刘春晓董瑞刘强
Owner MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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