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A kind of marine bacillus amyloliquefaciens and its application

A technology of amylolytic spores and bacilli, which is applied in the direction of bacteria, antibacterial drugs, and medical preparations containing active ingredients, etc. It can solve problems such as unsatisfactory effects, environmental pollution, and residual aquatic product species, achieving good development prospects and remarkable effects Effect

Active Publication Date: 2018-10-12
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, chemical agents are often used in the prevention and control of aquatic products, but the effect is not ideal, and it will pollute the environment and remain in aquatic species.

Method used

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  • A kind of marine bacillus amyloliquefaciens and its application
  • A kind of marine bacillus amyloliquefaciens and its application
  • A kind of marine bacillus amyloliquefaciens and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, the acquisition of marine bacillus amyloliquefaciens B01

[0040] 1. Collect samples: Collect the mud and seawater from sea cucumber culture ponds in Weihai, enrich and cultivate them for 20 days, then take them out and dilute them 1000 times, and culture them in an incubator at 28 °C for 24 hours.

[0041]2. Isolation of bacteria: Carefully pick out the bacteria with an inoculation needle, streak and purify them, and cultivate them in a 28 °C incubator. If bacterial colonies with different shapes, sizes and colors are found, they need to be picked separately for further purification until each plate present bacterial colonies of the same shape, size and color;

[0042] 3. Screening of antagonistic bacteria: Streak the above two aquatic pathogenic bacteria on the center of the 2216E medium plate, connect the purified bacteria at approximately equidistant four points around the pathogenic bacteria, cultivate them in an incubator at 28 °C, and observe the ...

Embodiment 2

[0060] Embodiment 2, the preparation of marine Bacillus amyloliquefaciens B01 fermentation metabolic liquid, the specific process is as follows:

[0061] 1. Preparation of marine Bacillus amyloliquefaciens B01 seed liquid: After activation, the marine Bacillus amyloliquefaciens B01 strain stored in a 4°C refrigerator was inoculated in LANDY liquid medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, Adjust the pH to 7.0-7.2, in 1000ml of water, sterilize at 121°C for 20min), and culture at 28°C with shaking at 150rpm / min for 12h, as the seed solution.

[0062] 2. Obtaining the fermentation and metabolism liquid of Bacillus amyloliquefaciens B01: the above seed liquid was inoculated into LANDY liquid medium at an inoculum size of 2%, and cultured by shaking at 150 rpm / min for 48 hours at 28°C to obtain a fermentation liquid. Centrifuge at 10000rpm / min for 10min, take the fermentation supernatant, filter it with a 0.2μm microporous membrane, and store it at -20°C f...

Embodiment 3

[0063] Embodiment three, fermented liquid (embodiment two) to the inhibitory effect of aquatic pathogenic bacteria:

[0064] The antagonistic activity of the fermentation supernatant was detected by agar diffusion method. Inoculate the above two aquatic pathogenic bacteria into the center of the 2216E medium plate by drawing a line, culture at 28 °C for a period of time, punch holes in the pathogenic bacteria with a hole puncher with a hole diameter of 8 mm, and number them, and add 100 µl B01 to each hole The fermentation supernatant of filter sterilization, 28 DEG C cultivates for a period of time, observes its antibacterial effect. The result is as image 3 shown.

[0065] It can be seen from the figure that the fermentation supernatant of marine Bacillus amyloliquefaciens B01 can effectively inhibit various aquatic disease bacteria such as Acinetobacter baumannii and Vibrio parahaemolyticus.

[0066] In summary, the present invention has isolated and obtained a strain o...

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Abstract

The invention discloses a strain of marine Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B01, which is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, with a preservation date of December 11, 2014 and a preservation number of CGMCC No.10154. The fermentation supernatant of marine Bacillus amyloliquefaciens B01 disclosed by the invention can inhibit aquatic diseases caused by various pathogenic bacteria such as Acinetobacter baumannii and Vibrio parahaemolyticus. The effect of preventing and controlling aquatic pollution diseases has a good prospect for the development of biological pesticides.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a marine bacillus amyloliquefaciens and its application. Background technique [0002] In recent decades, driven by the limitation of wild resources and market demand, my country's aquaculture industry has developed continuously and rapidly. It is currently the only country in the world where the production of aquaculture exceeds that of fishing. Aquaculture has become a major part of my country's national economy. The pillar industry continues to provide my country with a large amount of high-quality protein, and may become a key factor in solving my country's food security problems. [0003] With the rapid development of my country's aquaculture industry, large-scale and intensive farming models have gradually replaced extensive, semi-extensive, and semi-intensive farming models. The transformation of the breeding mode has brought disasters to the breeding industry while bringing hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/742A61P31/04C12R1/07
CPCA61K35/742C12N1/205C12R2001/07
Inventor 杜宗军穆大帅陈冠军
Owner SHANDONG UNIV
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