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Method and culture device for detecting yeasts and molds

A culturing device, yeast technology, applied to the culturing device of yeast or mold microorganisms, the field of detecting yeast and mold in samples, can solve the problem of low reproducibility, labor-intensive, extended type of mycelial growth hindering accurate counting of living cells, etc. question

Active Publication Date: 2015-10-07
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods, although widely accepted, have several disadvantages: These methods are generally labor-intensive and give low reproducibility
In addition, a common problem encountered in traditional methods is that the mycelial growth of some molds tends to extend beyond nearby colonies and prevent accurate counting of viable cells in the sample.

Method used

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  • Method and culture device for detecting yeasts and molds
  • Method and culture device for detecting yeasts and molds
  • Method and culture device for detecting yeasts and molds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment A

[0102] Embodiment A is a kind of cultivating device, and this cultivating device comprises:

[0103] a body comprising a self-supporting substrate having a first major surface and a second major surface;

[0104] a first adhesive composition disposed on a portion of the first major surface of the substrate;

[0105] a substantially dry, cold water soluble first hydrogel-forming composition attached to the first adhesive composition; and

[0106] Multiple indicators, said multiple indicators include:

[0107] Three indicators for detecting different glycosidase activities;

[0108] An indicator for detecting the enzyme activity of alkyl esterase;

[0109] Indicator for detecting phosphatase activity;

[0110] wherein each of said plurality of indicators comprises a detectable reporter group.

[0111] Embodiment B is the culture device of embodiment A, further comprising a cover sheet attached to the body member.

[0112] Embodiment C is the culture device of embodiment B,...

Embodiment W

[0132] Embodiment W is a method for detecting yeast and mold in a sample, the method comprising:

[0133] contacting the sample and the aqueous liquid with a gelling agent disposed on the first adhesive composition or the second adhesive composition of the culture device according to any one of embodiments A to V, to form an inoculated culture device;

[0134] incubating the inoculated culture device for a period of time; and

[0135] Detection of yeast or mold colonies in the culture device.

[0136] Embodiment X is the method of embodiment W, wherein detecting yeast or mold colonies in the culture device comprises detecting the presence of a detectable reporter group of at least one of a plurality of indicators in the culture device, wherein Detection of the presence of a detectable reporter group indicates the presence of colonies of yeast or mold microorganisms.

[0137] Embodiment Y is the method of embodiment W or embodiment X, wherein the sample is contacted with the...

example

[0145] indicator

[0146] Chromogenic substrate indicators used in the Examples are listed in Table 4 and were purchased from Biosynth International, Inc., Itasca, IL.

[0147] Table 4.

[0148] Indicator name

CAS number

5-Bromo-4-chloro-3-indole acetate (X-3-acetate)

3252-36-6

5-Bromo-4-chloro-3-indole-β-D-galactopyranoside (X-Gal)

7240-90-6

5-Bromo-4-chloro-3-indole-β-D-glucopyranoside (X-b-Glu)

15548-60-4

5-Bromo-4-chloro-3-indole-α-D-glucopyranoside (X-a-Glu)

108789-36-2

5-Bromo-4-chloro-3-indole phosphate p-toluidine salt (BCIP p-toluidine)

6578-06-9

[0149] Each of the indicator coating formulations A-D (Table 5) was composed of five chromogenic substrate indicators in the amounts specified in Table 5 with 100 g of a non-inhibiting binder of isooctyl acrylate and acrylamide Copolymers (described below) were prepared.

[0150] table 5.

[0151]

[0152] Medium formula

[0153] Six s...

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Abstract

A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.

Description

[0001] Cross references to related patent applications [0002] This application claims the benefit of US Patent Application Serial No. 13 / 775,495, filed February 25, 2013, and US Provisional Patent Application No. 61 / 760,412, filed February 4, 2013, which are hereby incorporated by reference in their entirety. Background technique [0003] Yeast and mold are eukaryotic microorganisms. They are ubiquitous in the natural environment, namely in soil, air, water and plant surfaces. Due to their heterotrophic nature and their ability to adapt to a wide range of environmental conditions, in many cases in a variety of commodities (including food ingredient ingredients, processed foods, beverages), food processing equipment that has not been adequately cleaned, and food storage facilities or encounter these microbes that are considered costly nuisances. In addition, some yeasts and molds have potential hazards to human and animal health. For example, many molds produce mycotoxins...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/00C12Q1/04G01N33/52
CPCC12Q1/04C12M23/04C12M23/20G01N33/52C12M23/24
Inventor 塞拉嘉·常德拉帕蒂特拉·M·诺德比
Owner 3M INNOVATIVE PROPERTIES CO
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