Recombined new castle disease virus vaccine strain for expressing African swine fever virus p72 proteins

Abstract

The invention provides a preparation method of a recombined new castle disease virus vaccine strain which expresses African swine fever virus p72 proteins and a recombined new castle disease virus vaccine strain. The method provided by the invention comprises the following steps: constructing a recombinant transcriptional plasmid which is inserted with a p72 gene of African swine fever virus (ASFV); constructing a transcriptional helper plasmid system; carrying out a contransfection for the transcriptional plasmids and the transcriptional helper plasmids into host cells BHK-21 which can be duplicated in new castle disease attenuate strains; and saving and obtaining the recombined virus stain. The vaccine strain for expressing the African swine fever virus p72 proteins provided by the invention has important preservation and strategy meaning in the aspect of animal epidemic disease prevention and control, and can be applied to the treatment and prevention of African swine fever virus.

Description

technical field [0001] The invention provides a preparation method of a recombinant Newcastle disease virus vaccine strain expressing African swine fever virus p72 protein and the recombinant Newcastle disease virus vaccine strain. Background technique [0002] African swine fever (ASF) is an acute, febrile, highly contagious disease caused by the African swine fever virus (ASFV), the only member of the African swine fever virus family [1]. Domestic pigs and European wild boars are generally susceptible, showing complex clinical symptoms, causing congestion, hemorrhage and dysfunction of the digestive and respiratory systems, and is a highly contagious disease in domestic pigs [2-4]. African wild boars usually show subclinical and no clinical symptoms of infection, and are the storage hosts of the disease; members of the genus Ornithodoros erraticus (Ornithodoros erraticus, Ornithodoros moubata) in soft ticks can serve as biological agents of the disease and storage hosts of...

AI Technical Summary

Problems solved by technology

There have been many reports on the research of African swine fever vaccine abroad, but traditional inactivated vaccines cannot produce effective immune protection against African swine fever virus infection

In 1960, during the outbreak of African swine fever in Portugal, a weakened strain was used for immunization prevention, and some immunized pigs became ill or even died. In addition, a large number of infected pigs appeared due to immunization, which was very unfavorable for the later eradication of the disease , and there is a risk of virus virulence becoming stronger after inoculation with attenuated African swine fever vaccine that has been weakened in passage

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