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A method for prenatal paternity determination using SNP

A paternity relationship and site technology, applied in the field of prenatal paternity relationship determination, can solve problems such as intrauterine infection and difficulty in amniocentesis, and achieve the effect of simple operation.

Active Publication Date: 2018-04-13
厦门万基生物科技有限公司
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AI Technical Summary

Problems solved by technology

However, amniocentesis is very difficult, and currently it needs to be completed under the guidance of B-ultrasound visual monitoring in top-tier hospitals and large hospitals, but there is still a 0.5%-1% risk of intrauterine infection and miscarriage

Method used

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  • A method for prenatal paternity determination using SNP
  • A method for prenatal paternity determination using SNP
  • A method for prenatal paternity determination using SNP

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Experimental program
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Effect test

Embodiment Construction

[0025] A method for determining prenatal parental relationship by using SNP of the present invention will be described in detail below with an example.

[0026] 1. Design loci: In the human genome, find SNP loci with a minimum allele frequency (MAF) of 0.4-0.5, and select a total of 1035 loci on the 15 chromosomes 1-13, 18, and 21 , the number of SNPs on each chromosome is similar. (The number of sites is variable during the design process, and the distribution of chromosomes is variable).

[0027] 2. Design primers: According to the designed 1035 SNP sites, use the ION AMPLISEQ DESIGNER online design website to design primers covering the target SNP. (Designing primers can be replaced by other software).

[0028] 3. Sample DNA extraction.

[0029] 4. Amplify fragments containing SNP sites: DNA fragments, multiplex PCR reaction enzymes and SNP Primers are mixed and then PCR reaction is performed.

[0030] 5. Purify the amplified PCR product.

[0031] 6. End repair: Mix th...

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Abstract

The invention provides a method for judging antenatal parental right relation with SNP. The SNP is used as a genetic marker, and judgment of the antenatal parental right relation is conducted through cooperation of the SNP and the high throughput sequencing technology. Only 10 ml of maternal peripheral blood is needed, free DNA extracted from the maternal peripheral blood plasma contains the free DNA of a fetus already, so that only one sample of a mother and the fetus is needed. Due to the fact that only the venous blood of a pregnant woman needs to be extracted, operation is simple and convenient, the pregnant woman and the fetus cannot be damaged, and the fetus can be identified after 10 weeks of pregnancy.

Description

technical field [0001] The invention relates to a method for judging prenatal parental relationship by using SNP. Background technique [0002] The paternity test is based on the theory and practice of human genetics, analyzes the genetic characteristics from the similar characteristics of the morphological structure or physiological function of the offspring and the parent, judges the suspicious father-son relationship or mother-child relationship, and makes affirmative or negative conclusions . [0003] The theoretical basis for judging the parent-child relationship is Mendel's law of segregation. According to this rule, when gametocytes are formed, paired alleles separate from each other and enter their respective gametocytes. Sperm and egg cells are fertilized to form offspring, and the child's two genomes are one from the mother and one from the father: therefore, the same pair of alleles is one from the mother and one from the father. If the identification results c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6869
CPCC12Q1/6869C12Q2531/113C12Q2535/122
Inventor 陈洪亮郑海灵段利朋祝兴强
Owner 厦门万基生物科技有限公司
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