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Application of betulinic acid in preparation of drugs for resisting fungus biofilms

A technology of betulinic acid and biofilm, which is applied in the field of medicinal chemistry and can solve problems that have not been reported

Active Publication Date: 2015-09-30
山东省阳信金缘纺化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there is no report on the antifungal biofilm effect of betulinic acid

Method used

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  • Application of betulinic acid in preparation of drugs for resisting fungus biofilms
  • Application of betulinic acid in preparation of drugs for resisting fungus biofilms
  • Application of betulinic acid in preparation of drugs for resisting fungus biofilms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The effect of embodiment 1 betulinic acid on Candida albicans biofilm

[0025] 1. Drugs

[0026] Betulinic acid: purchased from Shanghai Yiyan Biotechnology Co., Ltd.; fluconazole: purchased from Sigma; amphotericin B: purchased from Sigma; XTT was purchased from Sigma; dimethyl sulfoxide (DMSO) was purchased from Sigma ; PBS solution was purchased from Sigma

[0027] Betulinic acid was dissolved in DMSO, sterilized by filtering through a 0.22 μm filter before use, and stored in the dark at -20°C; amphotericin B and fluconazole were dissolved in DMSO solution respectively. XTT was made into a saturated solution of .5 mg / ml with sterile PBS buffer solution, and sterilized by filtering through a 0.22 μm filter; menadione was made into a 10M solution with 100% acetone, and XTT and menadione were stored at -80°C. Before the experiment, the drug solution was taken out and melted gradiently in an incubator.

[0028] 2. Experimental strains

[0029] Candida albicans SC5315...

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Abstract

The invention relates to application of betulinic acid and pharmaceutically acceptable salts thereof in preparation of drugs for resisting fungus biofilms. The invention provides a method for effectively resisting candida albicans biofilms. The adopted compound betulinic acid has effects of inhibiting the growth of candida albicans biofilms or inhibiting mature biofilms at a low concentration, and the minimal effective concentration of the compound betulinic acid is only 2.0 micron mol / L. The application of betulinic acid in preparation of drugs for resisting fungus biofilms, provided by the invention, not only provides a new application for betulinic acid, but also provides an effective drug for clinically inhibiting formation of fungus biofilms or damaging mature fungus biofilms.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to the application of betulinic acid in the preparation of antifungal biofilm drugs. Background technique [0002] Biofilm is a microbial aggregation group, which is a micro-ecological structure formed by microorganisms irreversibly contacting the surface of non-viable organisms or living tissues, and the extracellular matrix produced by themselves wraps living bacterial cells. Biofilms can be formed in the natural environment or in human hosts. They can protect microbial cells, resist various physical and chemical stimuli, and regulate gene expression. In daily life and industrial production, bacterial biofilms are ubiquitous, and most bacteria gather to form biofilms for production, which can grow in the protective film on the surface of various objects. As early as 1991, studies have shown that bacterial biofilms have a three-dimensional structure, and the bacterial ...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P31/10A01N45/00A01P3/00
Inventor 曹晓宏
Owner 山东省阳信金缘纺化有限公司
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