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A kind of bacillus amyloliquefaciens hn-11 and bacterial agent thereof

A technology of amylolytic spores and bacillus, applied in the direction of fungicides, bacteria, biocides, etc., can solve the problems of a small amount of ineffective effects, achieve huge economic and ecological benefits, good control effects, and great application prospects

Active Publication Date: 2018-12-25
深圳华农中绿生物科技研究有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The object of the invention is to provide a kind of Bacillus amyloliquefaciens HN-11 compatible with neem dregs and the preparation method of novel microbial agent thereof according to the above-mentioned deficiencies in the prior art. The effect is not good, and a large number of seedlings are injured, so the organic combination of bio-control bacteria and neem residue expands the spectrum of control

Method used

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  • A kind of bacillus amyloliquefaciens hn-11 and bacterial agent thereof
  • A kind of bacillus amyloliquefaciens hn-11 and bacterial agent thereof
  • A kind of bacillus amyloliquefaciens hn-11 and bacterial agent thereof

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Effect test

Embodiment 1

[0030] Enrichment Screening and Identification of Example 1 Bacillus amyloliquefaciens HN-11

[0031] 1. Enrichment screening of Bacillus amyloliquefaciens HN-11

[0032] The rhizosphere soil of neem tree collected from the herbarium of insecticidal plants of South China Agricultural University in Guangzhou City, Guangdong Province was used as the soil sample. Weigh 10g of soil and add neem residue medium (neem residue 30g, glucose 10g, peptone 5g, pH natural) for enrichment and culture at 28°C for one week, then weigh 10g of soil culture and put it into a triangular flask filled with 90mL sterile water After shaking fully, let stand for 10min. Take 1mL supernatant and dilute to 10-4, 10-5, 10-6 concentration according to 10-fold gradient concentration, take 0.1mL dilution respectively, spread on LB medium (peptone 10g, yeast powder 5g, NaCl10g, agar powder 15g of distilled water, 1L of distilled water, natural pH) on a plate, cultured upside down at 28°C for 2-3 days, and p...

Embodiment 2

[0047] Embodiment 2 Bacillus amyloliquefaciens HN-11 grows in the culture medium that takes neem residue as the sole carbon source

[0048] Pick a ring of bacteria liquid from the surface of the preserved Bacillus amyloliquefaciens HN-11 bacterial classification, then in the nutrient broth liquid medium (0.5% of beef extract, 0.5% of peptone, NaCl0.5%, 1.5% of agar, all the other are distilled water , pH7.2) on the plate, placed at 30 ° C for 24 hours; pick a single colony and inoculate it into a nutrient broth liquid medium (0.5% beef extract, 0.5% peptone, 0.5% NaCl, the rest is distilled water, pH7. In 2), shake the flask at 200 rpm at 30°C for 24 hours to obtain a seed solution.

[0049] The obtained seed solution was inserted into the liquid medium (neem residue 5g, peptone 10g, yeast powder 5g, distilled water 1L, pH natural) with neem residue as the only carbon source by 2% volume ratio, and glucose, sucrose, The culture medium with maltose and corn flour as the sole c...

Embodiment 3

[0053] Example 3 Bacillus amyloliquefaciens HN-11 has good compatibility with neem residue

[0054] Take 10g of neem residue, add methanol, ethyl acetate and 10mL of sterile water, mix well, extract in the dark for 2 days, centrifuge to get the supernatant, filter and sterilize through a 0.22μm sterile filter, and use Oxford The cup method was used to determine whether the extract had antibacterial activity against Bacillus amyloliquefaciens HN-11, and the extraction solvent was used as a control. The results showed that the methanol, ethyl acetate and water extracts of neem residue had no antibacterial activity against Bacillus amyloliquefaciens HN-11, which indicated that the strain HN-11 had good compatibility with neem residue.

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Abstract

The present invention relates to bacillus amyloliquefaciens and a preparation method of a microbial inoculum of the bacillus amyloliquefaciens, and belongs to the field of bio-pesticides and plant protection. The bacillus amyloliquefaciens HN-11 is isolated from azadirachta indica tree rhizosphere soil, azadirachta indica residue is used as a medium raw material and a microbial inoculum adsorption carrier for secondary solid state fermentation composting to prepare the microbial inoculums, the microbial inoculums comprises an arbitrary content of the bacillus amyloliquefaciens, 0 to 30% of the azadirachta indica residue, 4 to 12% of the soybean powder, 5 to 25% of wheat bran, 30-50% of peat, 1-5% of KCl, 0.5 to 2% of urea and 1 to 8% of a surfactant. Tests show that the microbial inoculum can overcome the problem of seedling injury caused by direct use of the azadirachta indica residue, can expand the control spectrum, has obvious integral control effect on banana wilt and Radopholus similes Thorne, can also promote plant growth, and the field control effect on the banana wilt can reach 96.2%. The microbial inoculum has great significance to the promotion of integral pest control and agricultural and sideline product recycling, and has broad application prospects in the production practice.

Description

technical field [0001] The invention belongs to the field of biopesticides and plant protection, in particular to a bacillus amyloliquefaciens bacterial strain ( Bacillus amyloliquefaciens ) HN-11 and its microbial agent preparation method, and its application in the control of plant diseases and insect pests. The strain was deposited in the China General Microorganism Culture Collection Management Center on October 31, 2013, with the preservation number CGMCC NO: 8421, and the preservation address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Background technique [0002] Neem residue is the by-product of the extraction of azadirachtin from the neem seeds of the insecticidal plant. Due to the low utilization rate, it is usually discarded as waste, which greatly wastes the raw materials that can be used as plant pest control products. Our research finds that neem slag itself still contains a small amount of active ingredients with insecticidal and antibacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P5/00C12R1/07
Inventor 徐汉虹赖多康向辉
Owner 深圳华农中绿生物科技研究有限公司
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