Lily culture method
A cultivation method and lily technology, applied in the field of plant cultivation, can solve the problems of species degradation, virus accumulation, low land utilization rate, etc., and achieve the effects of shortening the formation time, shortening the growth cycle, and shortening the cultivation cycle.
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Embodiment 1
[0032] Embryo selection and medium of edible lily (Songhualing No. 1):
[0033] Step a: Collect the capsules of Songhualing No. 1 30-60 days after pollination, wipe the surface three times with 75% alcohol cotton balls under sterile conditions, then soak in 0.1% mercury chloride solution for 15 minutes, and then rinse with sterile water 3 times. Cut the capsules in a sterile inoculation box, select the seeds with embryos to peel off the embryos, inoculate them on the medium MS+6-BA0.1mg / L+NAA0.2mg / L, and place them in the culture room for cultivation. The temperature is 24℃~27℃, the light is 3000Lx, and the light time is 12h / d;
[0034] Step b: Under aseptic conditions, peel off the scales of the seedlings obtained from the embryo culture, transplant to the proliferation medium of MS+NAA 0.01 mg / L+6-BA 0-1.0 mg / L and cultivate them. The concentrations of 6-BA are respectively 0 \0.25\0.5\0.75\1.0mg / L;
[0035] Step c: inoculate gained seedlings on the rooting medium of MS+6-...
Embodiment 2
[0038] Embryo selection and culture medium of Lily Lily (Iron Pao Lily):
[0039] Step a: Collect the capsules of Lilium japonica 30-60 days after pollination, wipe the surface with 75% alcohol cotton ball for 3 times under sterile conditions, soak in 0.1% mercuric chloride solution for 15 minutes, and then rinse with sterile water for 3 Second. Cut the capsules in the aseptic inoculation box, select the seeds with embryos to peel off the embryos, inoculate them on the medium MS+6-BA0.1mg / L+NAA0.2mg / L, place them in the cultivation room for cultivation, and the temperature The temperature is 24℃~27℃, the light is 3000Lx, and the light time is 12h / d;
[0040] Step b: Under aseptic conditions, peel off the scales of the seedlings obtained from the embryo culture, transplant to the proliferation medium of MS+NAA 0.01 mg / L+6-BA 0-1.0 mg / L and cultivate them. The concentrations of 6-BA are respectively 0 \0.25\0.5\0.75\1.0mg / L;
[0041]Step c: inoculate gained seedlings on the ro...
Embodiment 3
[0044] Embryo Selection and Medium of Ornamental Lily (Siberia):
[0045] Step a: collect the capsules 30-60 days after pollination in Siberia), wipe the surface with 75% alcohol cotton ball for 3 times under sterile conditions, soak in 0.1% mercuric chloride solution for 15 minutes, and then rinse with sterile water for 3 times .Cut the capsules in a sterile inoculation box, select the seeds with embryos to peel off the embryos, inoculate them on the medium MS+6-BA0.1mg / L+NAA0.2mg / L, place them in the culture room for cultivation, and the temperature is 24℃~27℃, light 3000Lx, light time 12h / d;
[0046] Step b: Under aseptic conditions, peel off the scales of the seedlings obtained from the embryo culture, transplant to the proliferation medium of MS+NAA 0.01 mg / L+6-BA 0-1.0 mg / L and cultivate them. The concentrations of 6-BA are respectively 0 \0.25\0.5\0.75\1.0mg / L;
[0047] Step c: inoculate gained seedlings on the rooting medium of MS+6-BA1.0mg / L+2.4-D0.5mg / L+NAA0~1.0mg / ...
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