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Preparation method of RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid)/modified hydroxyapatite porous composite material

A technology of porous composite materials and hydroxyapatite, which is applied in the fields of medical science and prostheses, can solve the problems of lack of cell identification signal, tissue aseptic necrosis, poor cell affinity, etc., and achieve simple manufacturing process and prevent sterile Bacterial necrosis, the effect of controlling the accumulation of acid

Inactive Publication Date: 2015-09-16
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural polymer materials have their own cell recognition signals, which are conducive to cell adhesion, good biocompatibility and cell affinity, but poor degradation performance
Synthetic polymers such as polylactic acid (PLA) have better biodegradability than natural polymer materials, but lack cell recognition signals, poor cell affinity, and acidic degradation, which can easily cause aseptic tissue necrosis

Method used

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  • Preparation method of RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid)/modified hydroxyapatite porous composite material
  • Preparation method of RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid)/modified hydroxyapatite porous composite material
  • Preparation method of RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid)/modified hydroxyapatite porous composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) First, dissolve 50ml of 5g polylactic acid (PDLLA), 0.5g maleic anhydride (MA), and 0.02g dibenzoyl peroxide (BPO) in dichloromethane, stir to make it evenly mixed, and vacuum-dry until constant Heavy, under the protection of nitrogen, high temperature melting reaction at 90 ℃ for 10h, THF-ethanol co-precipitation method to prepare maleic anhydride grafted polylactic acid MPLA;

[0026] 2) Dissolve 1g of the product MPLA and 0.0192g of hexamethylenediamine in 50ml and 5ml of tetrahydrofuran (THF) respectively, stir at a low temperature of 8°C, add the product MPLA dropwise in the tetrahydrofuran (THF) solution of hexamethylenediamine and react for 10min Rising to 28°C for 30 minutes, the mixed solution was dropped into excess distilled water to collect the surface film, and the product BMPLA modified by hexamethylenediamine was obtained. The infrared spectrum showed that it was at 1647cm -1 The characteristic absorption peak of the amide bond and the 1558cm -1 The -...

Embodiment 2

[0030] 1) First, dissolve 50ml of 5g polylactic acid (PDLLA), 0.5g maleic anhydride (MA), and 0.02g dibenzoyl peroxide (BPO) in dichloromethane, stir to make it evenly mixed, and vacuum-dry until constant Heavy, under the protection of nitrogen, high temperature melting reaction at 90 ℃ for 10h, THF-ethanol co-precipitation method to prepare maleic anhydride grafted polylactic acid MPLA;

[0031] 2) Dissolve 1g of the product MPLA and 0.0128g of hexamethylenediamine in 50ml and 5ml of tetrahydrofuran (THF) respectively, stir at a low temperature of 20°C, add the product MPLA dropwise in the tetrahydrofuran (THF) solution of hexamethylenediamine and react for 10min Rising to 28°C for 30 minutes, the mixed solution was dropped into excess distilled water to collect the surface film, and the hexamethylenediamine-modified product BMPLA was obtained;

[0032]3) Dissolve 1 g of product BMPLA in 50 ml of THF, adjust the pH, add 0.15 g of 1-ethyl-(3-dimethylaminopropyl) carbodiimide h...

Embodiment 3

[0035] 1) First, dissolve 50ml of 5g polylactic acid (PDLLA), 0.5g maleic anhydride (MA), and 0.02g dibenzoyl peroxide (BPO) in dichloromethane, stir to make it evenly mixed, and vacuum-dry until constant Heavy, under the protection of nitrogen, high temperature melting reaction at 90 ℃ for 10h, THF-ethanol co-precipitation method to prepare maleic anhydride grafted polylactic acid MPLA;

[0036] 2) Dissolve 1g of the product MPLA and 0.0159g of hexamethylenediamine in 50ml and 5ml of tetrahydrofuran (THF) respectively, stir at a low temperature of 8°C, add the product MPLA dropwise in the tetrahydrofuran (THF) solution of hexamethylenediamine and react for 30min Rising to 28°C for 1 hour, the mixed solution was dropped into excess distilled water to collect the surface film, and the hexamethylenediamine-modified product BMPLA was obtained;

[0037] 3) Dissolve 1g of the product BMPLA in 50ml of THF, adjust the pH, add 0.15g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydro...

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Abstract

The invention relates to a preparation method of an RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid) / modified hydroxyapatite porous composite material. The preparation method comprises the following steps: (1) introducing maleic anhydride and hexamethylendiamine to a side chain of polylactic acid at first, and then grafting adhesive RGD polypeptide; (2) dissolving the generated RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid) in dichloromethane, adding modified nano modified hydroxyapatite, performing ultrasonic dispersion, stirring fully to mix uniformly, weighing sodium chloride particles screened by a molecular sieve, adding the sodium chloride particles into the mixed solution and stirring; and (3) when a solvent is volatilized, drying to perform volatilization, and soaking the material in distilled water to separate out the sodium chloride particles, thereby obtaining the RGD polypeptide grafted poly(maleic anhydride-hexamethylendiamine-DL-lactic acid) / modified hydroxyapatite porous composite material at last. The preparation method provided by the invention has the advantages that a manufacturing process is simple and controllable.

Description

technical field [0001] The invention relates to a preparation method of RGD polypeptide grafted poly(maleic anhydride-hexamethylenediamine-DL-lactic acid) / modified hydroxyapatite porous composite material which can be used for repairing tissue defects. Background technique [0002] In the field of biomedical materials, in recent years, special attention has been paid to degradable biopolymer materials, which have excellent mechanical properties and controllable degradation cycle. The small molecules produced by hydrolysis or enzymatic hydrolysis during the degradation process can be absorbed or excreted by the human body. Biopolymer materials that can cause harm to the human body are increasingly favored by people. Natural polymer materials have their own cell recognition signals, which are conducive to cell adhesion, good biocompatibility and cell affinity, but poor degradation performance. Synthetic polymers such as polylactic acid (PLA) have better biodegradability than ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08L67/04C08K9/06C08K3/32C08G63/91A61L27/46A61L27/18A61L27/22A61L27/58A61L27/56
Inventor 王友法于晓
Owner WUHAN UNIV OF TECH
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