Method for extracting Choerospondin

A technology of jujube jujube and its structural formula, which is applied in the field of extracting active ingredients of traditional Chinese medicine, and can solve the problems of less research on jujube jujube jujube

Inactive Publication Date: 2015-09-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are few studies on zyoside, and through literature search, it has not ...

Method used

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  • Method for extracting Choerospondin

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Embodiment 1

[0021] Take 1kg of southern jujube bark and pulverize it into a coarse powder, add it to a supercritical extraction kettle, and carry out supercritical CO extraction with ethyl acetate as an entraining agent. 2 Extraction, CO 2 The flow rate is 3.5ml / g crude drug / min, the amount of entrainer is 12% of the total extraction solvent, the extract is purified by neutral magnesium oxide column chromatography, the neutral magnesium oxide is 200 mesh, and the magnesium oxide column is a medium and low pressure column. The column pressure is 6.5bar, the column diameter to height ratio is 1:12, petroleum ether-methanol mixed solvent gradient elution, petroleum ether-methanol volume ratio is 20:1-1:1, the eluate is collected and concentrated under reduced pressure, and dried at low temperature to obtain The crude product was dissolved in methanol, injected into a preparative high-performance liquid chromatograph, 35% acetonitrile solution was used as the mobile phase, and the ultraviolet...

Embodiment 2

[0023] Take 1kg of southern jujube bark and pulverize it into a coarse powder, add it to a supercritical extraction kettle, and carry out supercritical CO extraction with ethyl acetate as an entraining agent. 2 Extraction, CO 2 The flow rate is 3.0ml / g crude drug / min, the amount of entrainer is 15% of the total extraction solvent, the extract is purified by neutral magnesium oxide column chromatography, the neutral magnesium oxide is 200 mesh, and the magnesium oxide column is a medium and low pressure column. The column pressure is 6bar, the column diameter to height ratio is 1:12, the petroleum ether-methanol mixed solvent gradient elution, the petroleum ether-methanol volume ratio is 20:1-1:1, the eluate is collected and concentrated under reduced pressure, and dried at low temperature to obtain the crude product , dissolved in methanol, injected into a preparative high-performance liquid chromatograph, 35% acetonitrile solution as the mobile phase, ultraviolet on-line dete...

Embodiment 3

[0025] Take 1kg of southern jujube bark and pulverize it into a coarse powder, add it to a supercritical extraction kettle, and carry out supercritical CO extraction with ethyl acetate as an entraining agent. 2 Extraction, CO 2 The flow rate is 4.0ml / g crude drug / min, the amount of entrainer is 16% of the total extraction solvent, the extract is purified by neutral magnesium oxide column chromatography, the neutral magnesium oxide is 200 mesh, and the magnesium oxide column is a medium and low pressure column. The column pressure is 7bar, the column diameter-to-height ratio is 1:12, the petroleum ether-methanol mixed solvent gradient elution, the petroleum ether-methanol volume ratio is 20:1-1:1, the eluate is collected and concentrated under reduced pressure, and dried at low temperature to obtain the crude product , dissolved in methanol, injected into a preparative high-performance liquid chromatograph, 35% acetonitrile solution as the mobile phase, ultraviolet on-line dete...

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Abstract

The invention provides a preparation method for Choerospondin and application of the Choerospondin in antibacterial drugs. The method comprises the following steps: using barks of Choerospondias axillaris as a raw material, carrying out crushing, adding crushed barks into a supercritical extraction kettle, and using ethyl acetate as an entrainer and then carrying out supercritical CO2 extraction; purifying obtained extract through neutral magnesium oxide column chromatography, carrying out gradient elution by using a mixed solution of petroleum ether and methanol, then collecting obtained eluent, and carrying out pressure-reduced condensation and low-temperature drying; and carrying out preparative highly-efficient liquid-phase chromatographic separation so as to obtain the Choerospondin. According to the invention, pharmacological experiments show that the Choerospondin has certain antibacterial activity against a plurality of bacterium.

Description

technical field [0001] The invention belongs to the field of extracting effective components of traditional Chinese medicines, and relates to a method for extracting jujube glycosides. Background technique [0002] Choerospondin is a flavonoid glycoside compound, which is mainly isolated from the bark of Choerospondias axillaris (Roxb.) Burtt et Hill, Anacardiaceae. Pharmacological studies have shown that jujube glycoside has antibacterial effect. It has inhibitory effect on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and nitrate-negative bacilli; it also has inhibitory effect on aldose reductase. The molecular formula of jujube glycoside is C 21 h 22 o 10 , the molecular weight is 434.4, and the structural formula is: [0003] . [0004] At present, there are few studies on zyoside, and through literature search, there is no domestic use of zyoside in the preparation of antibacterial drugs or health care products. Contents of the invention [...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H1/08A61K31/7048A61P31/04
CPCY02A50/30Y02P20/54
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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