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Jettisonable one-step electro-deposition immune-biosensor for detecting histamine and preparation method of immune-biosensor

A biosensor and electrodeposition technology, applied in the field of biosensors, can solve the problems of small molecular weight of histamine, only one antigen active site, and it is difficult to directly detect histamine, and achieve good viscosity, good electrocatalytic activity, and obvious stability sexual effect

Active Publication Date: 2015-09-09
深圳深镧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight of histamine is extremely small (111 Da) and there is only one antigenic active site, so it is difficult to directly detect histamine using an electrochemical immunosensor

Method used

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  • Jettisonable one-step electro-deposition immune-biosensor for detecting histamine and preparation method of immune-biosensor
  • Jettisonable one-step electro-deposition immune-biosensor for detecting histamine and preparation method of immune-biosensor
  • Jettisonable one-step electro-deposition immune-biosensor for detecting histamine and preparation method of immune-biosensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This embodiment provides a disposable one-step electrodeposition immunobiological sensor for detecting histamine, which uses an electrode with a Prussian blue-chitosan-nano-gold composite film on the surface, and the histamine coating agent is immobilized on the electrode.

[0045] Nano gold and Prussian blue (Fe 4 [Fe(CN) 6 ] 3 ) molar concentration ratio of 1:1000, chitosan content of 0.05%.

[0046] A preparation method of the above immune biosensor, comprising the steps of:

[0047] S1. Dissolve 0.05g chitosan in 100 mL of 1.0% acetic acid solution, stir at room temperature for 3 hours to completely dissolve chitosan, and prepare a 0.05% chitosan solution. 0.0406 g FeCl 3 , 0.0822g K 3 [Fe(CN) 6 ], 0.7455g KCl and 1mL concentrated hydrochloric acid were added to 100 mL of the above-mentioned chitosan solution, and ultrasonically dispersed at room temperature until a stable dark green dispersion was obtained; solution, and then ultrasonically disperse at room ...

Embodiment 2

[0055] This embodiment provides a disposable one-step electrodeposition immunobiological sensor for detecting histamine, which uses an electrode with a Prussian blue-chitosan-nano-gold composite film on the surface, and the histamine coating agent is immobilized on the electrode.

[0056] Nano gold and Prussian blue (Fe 4 [Fe(CN) 6 ] 3 ) The molar concentration ratio is 1:2000, and the chitosan content is 0.05%.

[0057] A preparation method of the above immune biosensor, comprising the steps of:

[0058] S1. Dissolve 0.05 g chitosan in 100 mL 1.0% acetic acid solution, stir at room temperature for 3 h to completely dissolve chitosan, and prepare a 0.05% chitosan solution. 0.0813 g FeCl 3 , 0.1644 g K 3 [Fe(CN) 6 ], 0.7455g KCl and 1 mL of concentrated hydrochloric acid were added to 100 mL of the above chitosan solution, and ultrasonically dispersed at room temperature until a stable dark green dispersion was obtained; In the above solution, ultrasonically disperse at ...

Embodiment 3

[0065] This embodiment provides a disposable one-step electrodeposition immunobiological sensor for detecting histamine, which uses an electrode with a Prussian blue-chitosan-nano-gold composite film on the surface, and the histamine coating agent is immobilized on the electrode.

[0066] Nano gold and Prussian blue (Fe 4 [Fe(CN) 6 ] 3 ) molar concentration ratio of 1:400, chitosan content of 0.01%

[0067] A preparation method of the above immune biosensor, comprising the steps of:

[0068] S1. Dissolve 0.01g chitosan in 100 mL of 1.0% acetic acid solution, stir at room temperature for 2 h to completely dissolve chitosan, and prepare a 0.01% chitosan solution. 0.0163 gFeCl 3 , 0.0329 g K 3 [Fe(CN) 6 ], 0.7455 g KCl and 1 mL of concentrated hydrochloric acid were added to 100 mL of the above chitosan solution, and ultrasonically dispersed at room temperature until a stable dark green dispersion was obtained; then 1 mL of prepared nano-gold colloid was added to the mixed ...

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Abstract

The invention belongs to the technical field of biosensors, and specifically discloses a jettisonable one-step electro-deposition immune-biosensor for detecting histamine and a preparation method of the immune-biosensor. The immune-biosensor adopts an electrode coated with a prussian blue, chitosan and nanogold composite film, wherein histamine coating antigen is fixed on the electrode, the molar concentration ratio of nanogold to prussian blue in the composite film is 1:2000 to 1:20, and the massic volume content of chitosan is 0.01-0.05%. Furthermore, the composite film is formed on the surface of the electrode through a one-step electrode deposition manner, the deposition step is simpler than a preparation step of the conventional electrode modified by high-molecular polymer, and the antigen binding site can be easily exposed out. According to the electro-deposition immune-biosensor, competition immunoreaction and a biosensor are combined, and a series of property characteristics such as a histamine detection working curve can be obtained according to needs, and the jettisonable one-step electro-deposition immune-biosensor has the advantages of simplicity in operation, high sensitivity, rapidness, convenience and the like, and can be used for on-site rapid detection for histamine in a sample.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and more specifically relates to a throwable one-step electrodeposition immunobiological sensor for detecting histamine and a preparation method thereof. Background technique [0002] my country is rich in fish resources and is also a big country in the production and consumption of aquatic products. Aquatic products and their processing play an important role in the national economy and consumption. Histamine is formed by the decarboxylation reaction of free histidine under the catalysis of histidine decarboxylase. It is a toxic biogenic amine and widely exists in various organisms and foods, mainly aquatic products. Especially pelagic fish with green skin and red meat and their processed products. Studies have shown that excessive intake of histamine can cause a series of allergic reactions such as headache, nausea, nausea and vomiting, diarrhea, heart palpitations, itchy rash, and changes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N33/53
Inventor 徐振林孙远明董秀秀梁祖培沈玉栋杨金易王弘雷红涛
Owner 深圳深镧科技有限公司
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