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Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles

A technology for water purification and bacteria, applied in chemical instruments and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc. External environmental damage, poor mechanical properties of particles, etc., to achieve the effect of reducing bacterial loss, shortening start-up time, and enhancing stability

Active Publication Date: 2015-09-09
CENT LAB FUJIAN ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing preparation method of bacteria-immobilized granules is difficult to realize industrialized production. Poor performance, weak settling performance, easy loss, etc., provide a kind of bacteria-immobilized particles for water purification and its preparation method, the bacteria-immobilized particles prepared by the method of the present invention can effectively maintain a high concentration of bacteria and biological activity, Enhanced poison resistance, short start-up time, fast reaction speed, less microbial loss, and efficient, durable, and stable treatment of various types of sewage; at the same time, the preparation process of the present invention is simple, can be mass-produced with high efficiency, and has good mechanical properties for immobilization of bacteria particles

Method used

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  • Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles
  • Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles
  • Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles

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Embodiment 1

[0037] Bacterial liquid: Bacillus subtilis liquid.

[0038] The preparation method of bacillus subtilis immobilized granules comprises the following preparation steps:

[0039] (1) Add 1.6 kg of activated carbon and 0.4 kg of diatomaceous earth to 18 L of bacteria liquid in the late logarithmic growth period, stir and adsorb for 20 min, and prepare Bacillus subtilis liquid;

[0040] (2) Add 160 mL of 12wt% chitosan acetic acid solution to the microbial bacterial liquid prepared in step (1), first stir rapidly for 20 min, then slowly stir for 30 min, then let it stand for 6 h, and then suck it up with a siphon supernatant to make 3.8 kg of bacterial concentrate;

[0041] (3) Add 2.4 kg of diatomaceous earth, 0.8 kg of sodium bentonite and 0.8 kg of silicon dioxide to the bacterial concentrate prepared in step (2), and stir for 20 minutes;

[0042] (4) Add 6.24 L (15wt%PVA+0.75wt%SA) mixed solution to the bacterial mixture material obtained in step (3), and use a mixer to stir...

Embodiment 2

[0050] The microbial bacterial liquid is a mixture of Bacillus subtilis, Bacillus thuringiensis, yeast and photosynthetic bacteria.

[0051] The preparation method of mixed bacteria-immobilized particles comprises the following preparation steps:

[0052] (1) Add 200 g of activated carbon and 50 g of diatomaceous earth to 2L of bacterial mixture in the late logarithmic growth period (the mixing ratio of Bacillus subtilis, Bacillus thuringiensis, yeast and photosynthetic bacteria is 2:1:1:1) , stirred and adsorbed for 20 minutes to obtain the microbial bacterial liquid;

[0053] (2) Add 20 mL of 12wt% chitosan acetic acid solution to the microbial bacterial solution prepared in step (1), stir rapidly for 20 min, then slowly stir for 30 min, let stand for 6 h, and then use a siphon to absorb the Clear liquid, make the bacterial concentrate of 450 g;

[0054] (3) Add 300 g of diatomite, 100 g of sodium bentonite and 100 g of silicon dioxide to the bacterial concentrate prepared...

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Abstract

The invention discloses bacteria immobilization particles for water purification and a preparation method of the bacteria immobilization particles, and belongs to the technical field of water treatment. The preparation method comprises the following steps: preparing a bacteria concentrate through flocculation by utilizing activated carbon, diatomite and a chitosan acetic acid solution; adding diatomite, bentonite, silicon dioxide and a polyvinyl alcohol-sodium alginate mixed solution into the concentrate for stirring; carrying out granulation with a granulator to prepare the bacteria immobilization particles. The prepared bacteria immobilization particles are high in biological activity, high in bacteria content (5*10<9>-1*10<10> CFU / g), short in starting time, and high in reaction speed, has a honeycomb structure (100-300 m<2> / g in specific surface area) suitable for bacteria apposition growth and mass transfer, and can be used for immobilizing bacteria for long, prohibiting the invasion of external hazardous materials, and permanently and efficiently treating sewage in different environments and of different water quality types; meanwhile, industrialized production is available for a whole bacteria immobilization particle preparation process, and the prepared bacteria immobilization particles are high in mechanical property, long in service life, and convenient to store and convey.

Description

technical field [0001] The invention belongs to the technical field of water treatment, in particular to a bacteria-immobilized particle for water purification and a preparation method thereof. Background technique [0002] Immobilized microorganism technology is a method that uses physical or chemical means to limit free microbial cells to a certain space area, so that microorganisms can maintain high density and activity and can be used repeatedly. Compared with the traditional free microorganism treatment method, the immobilized microorganism technology can greatly increase the concentration of microorganisms, so that the microorganisms are not easy to be lost and polluted, the use time is long, the anti-toxicity and tolerance are significantly increased, and the solid-liquid separation is easy, and the secondary pollution is less. . These advantages make immobilized microorganism technology have broad application prospects in the field of water treatment. [0003] Curr...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/04C02F3/34
Inventor 黄薇宋永康林代炎陈丽华刘文静王命福
Owner CENT LAB FUJIAN ACADEMY OF AGRI SCI
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