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Honeybee body surface parasitic mite control method and mite repellent

A technology for repelling mites and parasitic mites, applied in the field of biology, can solve problems such as allergic and poisoning reactions, and achieve the effects of strong specificity, environmental friendliness and strong specificity

Inactive Publication Date: 2015-09-09
XIAN JIAOTONG LIVERPOOL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first problem to be solved by the present invention is to overcome the problems of allergies and poisoning reactions caused by existing acaricides, and provide a method for controlling honeybee epiparasitic mites, which is simple, sensitive and specific

Method used

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  • Honeybee body surface parasitic mite control method and mite repellent
  • Honeybee body surface parasitic mite control method and mite repellent
  • Honeybee body surface parasitic mite control method and mite repellent

Examples

Experimental program
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Effect test

Embodiment 1

[0131] Concentration test of embodiment 1 α-terpineol in bee colony infected by Varroa mite

[0132] 30 Varroa mites were collected in a test container (diameter 90mm). The test container is a circular piece of paper wetted with 5 μl of DMSO and α-terpineol and divided into two parts from the middle (C: DMSO area, T: α-terpineol area). After 5 minutes, the mites were evenly distributed in the two parts; but after 50 minutes, more mites were concentrated in area C. If we replace the α-terpineol in region T with DMSO, the above phenomenon will not be observed.

[0133] We obtained the number of mites in the two areas and calculated the avoidance coefficient by (C-T) / (C+T) (where C and T represent the number of mites in the corresponding area after 5 minutes). Data median ± SEM (n=3). In addition to 0.03M, the concentration of α-terpineol was 0.3M, 0.5M and 1.0M, and the avoidance response of Varroa mites was clearly observed. P values ​​corresponding to the 3 concentrations:...

Embodiment 2

[0134] Field test of embodiment 2 α-terpineol in bee colony infected by Varroa mite

[0135] Then we tested the anti-mite effect of α-terpineol by field test. We first selected a bee colony infected by Varroa mites, then added 1 μl of 0.3M α-terpineol to each worker bee hive containing L5 larvae, and added 1 μl of DMSO to the same number of hives as an internal reference. Two days later, the hives were opened to check the number of mites parasitizing the hives. This experiment was repeated 4 times on different combs within the same colony, and a total of 320 hives were tested. Compared with the control group, the number of parasitized hives added with α-terpineol was significantly less than 37% (internal reference hive parasitism=42.5%, hive parasitized with α-terpineol=26.6%; X2=18.26, P<0.0001, Mantel -Haenszel detection). This result actually verifies that α-terpineol can inhibit the parasitism of Varroa mites on worker bee larvae. Therefore, similarly, α-terpineol and / ...

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Abstract

The invention provides a honeybee body surface parasitic mite control method. A natural compound in plant essential oil, capable of activating TRP protein channels, is screened out with the TRP channels of mites as target sites. The natural compound is used to stimulate and activate the TRP channels of the mites in order to induce aversion behavior of the mites, so the mite repelling purpose is achieved.

Description

technical field [0001] The invention relates to the technical field of biology, in particular, the invention relates to an acaricide and a method for controlling ectoparasitic mites of bees. Background technique [0002] The health of bees is threatened by a wide variety of pathogens such as viruses, bacteria, microsporidia and protists. The decline in bee populations caused by these pathogens has caused severe economic losses to beekeepers and those who need bees for pollination. However, mites that are ectoparasitic on bees have a more serious impact on bee health. In all continents with beekeeping, except Australia, Varroa desces is the main pest that threatens the health of bees. In most countries in Asia, the same degree of damage is caused by the mite. They can not only cause the death of bee larvae and bee pupae by sucking lymph, but also can indirectly transmit viruses and other bee pathogens. [0003] Most of the acaricides commonly used to control these two typ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/045A61K31/085A61K31/35A61K31/05A61K31/215A61K31/121A61K31/122A61K31/11A61K31/105A61P33/14
Inventor 门脇辰彦彭广大董晓峰李天邦朱静婷森本智美加塩麻纪子富永真琴
Owner XIAN JIAOTONG LIVERPOOL UNIV
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