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Diluent for hog cholera live vaccine (spleen and lymph tissue origin)

A swine fever spleen shower source and diluent technology, which is applied to medical preparations containing active ingredients, plant raw materials, tripeptide ingredients, etc., can solve the problems of unsatisfactory immune effect, uneven quality, and improper use methods. To enhance the ability of B cells to produce and secrete antibodies, improve the use effect, and promote the effect of differentiation

Inactive Publication Date: 2015-09-02
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the market believes that the immunization effect of the live vaccine of classical swine fever spleen and lymph is not ideal, and there are still misunderstandings about the live vaccine of classical swine fever and spleen. Excessive stress and uneven quality

Method used

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  • Diluent for hog cholera live vaccine (spleen and lymph tissue origin)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of Chinese medicine polysaccharide and Chinese medicine flavone

[0030] a. According to 20 parts of forsythia, 20 parts of ginseng, 10 parts of Guanzhong, 10 parts of thistle, 30 parts of Burnet, 10 parts of mountain bean root, 10 parts of antelope horn and 20 parts of Viola chinensis, After chopping and washing, soak in cold water overnight, then add purified water 15 times the weight of raw materials, water bath to 90°C, and keep at 90°C, cook for 2 hours, stir every 10 minutes during the cooking process;

[0031] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0032] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, and the supernatant is the crude Chinese medicine flavonoid;

[0033...

Embodiment 2

[0038] Embodiment 2: the preparation of bursalin

[0039] a. Take healthy bursa tissue, remove the fascia and adipose tissue from the bursa tissue, and wash it with sterilized cold PBS at pH 7.2.

[0040] b. Weigh the bursa of Fabricius, add PH7.2 sterilized cold PBS at a ratio of 1:1 (W:V), and perform high-speed homogenization in a tissue homogenizer.

[0041] c. Add 2.5% trypsin to the homogenate, freeze and thaw three times, centrifuge at 12000rpm for 20min, and discard the precipitate.

[0042] d. The supernatant is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000da, and the liquid under the membrane is the crude extract of bursalin.

[0043] e. The crude extract in d is sterilized by filtration through a 0.22um filter membrane, and then freeze-dried in a vacuum to make bursalin freeze-dried powder.

Embodiment 3

[0044] Embodiment 3: the preparation of a kind of diluent for swine fever splenic gonorrhea source live vaccine

[0045] Add 25g of traditional Chinese medicine polysaccharide, 40g of traditional Chinese medicine flavonoids, 7g of vitamins (70mg of D-biotin, 30mg of folic acid, 4g of nicotinamide and 2.9g of vitamin E) and 20mg of bursalin in every 1000ml of phosphate buffer solution, and then get swine fever splenosis. Diluents for source live vaccines. Among them, the polysaccharide of traditional Chinese medicine and the flavonoid of traditional Chinese medicine are prepared according to the steps in Example 1, and the bursalin is prepared according to the steps in Example 2. Phosphate buffer solution consists of 6-10g sodium chloride, 0.05-0.5g potassium chloride, 1-1.2g disodium hydrogen phosphate, 0.05-0.5g potassium dihydrogen phosphate, 0.05-0.2g calcium chloride, containing 6 crystal water Dissolve 0.05-0.2g of magnesium chloride in 1000ml of double-distilled water a...

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Abstract

The invention relates to a diluent for a hog cholera live vaccine (spleen and lymph tissue origin), belonging to the technical field of veterinary biological products. Every 1000ml of phosphate buffer solution of the diluent contains 15-50g of Chinese medicine polysaccharide, 20-60g of Chinese medicine flavone, 5-10g of vitamin and 5-40mg of bursin, wherein the Chinese medicine polysaccharide and the Chinese medicine flavone are extracted from the following Chinese medicine compositions: fructus forsythia, bistort, cyrtomium fortunei, cirsium japonicum, sanguisorba officinalis, subprostrate sophora, antelope's horn and Chinese violet. After an organism is immunized by the vaccine prepared from the diluent, differentiation and proliferation of a B lymphocyte precursor of the organism can be facilitated, the speed of transcribing DNA (deoxyribonucleic acid) into mRNA (Messenger Ribonucleic Acid) in B lymphocyte is accelerated, the generation of proteins in the B lymphocyte is promoted, and thus the generation and antibody secretion capabilities of the B lymphocyte are enhanced, and the immunity of the organism can be improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a diluent for a live vaccine of swine fever and splenic gonorrhea. Background technique [0002] Classical swine fever (CSF) is a Class A legal infectious disease of the International Organization for Animal Health (OIE) and China. It is one of the major animal diseases that are subject to compulsory immunization in my country, and is the "first killer" that endangers my country's pig industry. Swine fever is caused by swine fever virus and cannot be controlled with drugs. Vaccination is the most effective way. [0003] At present, the CSF vaccines on the market at home and abroad mainly include CSF splenic vaccine, CSF cell vaccine and CSF subunit vaccine. As a classical-refined live vaccine of classical swine fever and splenic gonorrhea in vaccines, it still has an irreplaceable role. [0004] The Chinese swine fever splenic and gonorrhea li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K38/06A61P37/04A61K36/86A61K31/715A61K35/32A61K31/4188A61K31/519A61K31/455A61K31/355
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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