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Fermented soybean meal, bacterial strain and application thereof

A technology for fermenting soybean meal and soybean meal, applied in application, fungi, climate change adaptation, etc., can solve the problems of high content of anti-nutritional factors, low protein utilization rate, low protein content, etc., and achieve low content of anti-nutritional factors, small peptides The effect of high content and high protein content

Active Publication Date: 2018-01-12
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing fermented soybean meal with high protein content, a new bacterial strain for preparing fermented soybean meal---Awamori Aspergillus strain42 (Aspergillus awamori strain42), and the application of fermented soybean meal in the preparation of carnivorous fish feed, It is used to solve the problems of low protein content, low protein utilization rate, high anti-nutritional factor content and poor palatability in the process of replacing fish meal with plant protein sources, so as to make up for the deficiencies of the existing technology

Method used

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  • Fermented soybean meal, bacterial strain and application thereof
  • Fermented soybean meal, bacterial strain and application thereof
  • Fermented soybean meal, bacterial strain and application thereof

Examples

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Effect test

Embodiment 1

[0023] The expanded cultivation of embodiment 1 Aspergillus awamori

[0024] Inoculate Aspergillus awamori strain42 into PDA medium and culture at 25-30°C for 2-3 days to obtain slant bacteria; inoculate slant bacteria to seed medium and culture at 25-30°C for 2-3 days to expand The final Aspergillus awamori spores are washed with sterile water, the spores are collected, and the spores are prepared into a spore suspension with sterile water; the final concentration of the seed medium is composed of: bran 1000g / L, soybean meal 200g / L, solvent is water, pH nature. The composition of PDA medium is: potato 200g / L, glucose 20g / L, agar 15-20g / L, solvent is tap water, pH is natural.

Embodiment 2

[0025] The preparation of embodiment 2 fermented soybean meal

[0026] (1) Soak 1kg of soybean meal in 0.5L nutrient solution, soak at 25°C for 15min, and sterilize in a cabinet type sterilizer at 100°C for 20min to obtain the fermentation substrate; the final concentration of the nutrient solution is composed of: K 2 HPO 4 7g / L, NaCl 5g / L, (NH 4 ) 2 SO 4 4g / L, glucose 6g / L, solvent is water.

[0027] (2) According to the ratio of inoculating 1.5g wet weight of spores per kilogram of soybean meal (dry weight), inoculate the spore suspension prepared in Example 1 into the fermentation substrate of step (1), at a temperature of 28-30°C and a humidity of 100%. After 33 hours of fermentation, the culture was taken out, dried at 50°C, crushed, and passed through an 80-mesh sieve to obtain fermented soybean meal.

Embodiment 3

[0029] (1) Soak 1kg of soybean meal in 0.5L nutrient solution, soak at 25°C for 15min, and sterilize in a cabinet type sterilizer at 100°C for 20min to obtain the fermentation substrate; the final concentration of the nutrient solution is composed of: K 2 HPO4 5 / L, NaCl 3 / L, (NH 4 ) 2 SO 4 4 / L, glucose 6g / L, solvent is water.

[0030] (2) According to the ratio of inoculating 1.5g wet weight of spores per kilogram of dry weight of soybean meal, the spore suspension prepared in Example 1 was inoculated into the fermentation substrate, and fermented and cultivated at 28° C. and 100% humidity respectively for 0, 24, and 33, 42, 51, 60 hours, 3 repetitions for each treatment. After the cultivation, the culture was taken out, dried at 50°C, crushed, and passed through an 80-mesh sieve to obtain fermented soybean meal. The protein content of fermented soybean meal in each group was detected by the Kjeldahl method, and the results are shown in figure 1 As shown, it was found tha...

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Abstract

The present invention discloses a fermented soybean meal, a strain for preparing the fermented soybean meal, and an application of the fermented soybean meal in preparing carnivorous fish feed additives. The prepared fermented soybean meal is characterized by having a high content of protein, a high content of small molecules such as free amino acids and small peptide, a low content of anti-nutritional factors, a fermented flavor, and a good palatability. According to the fermented soybean meal, the content of crude protein is increased to 60.5-61.7%, the content of the small peptide is increased from 3.9-4.3% to 18.6-19.8%, a soybean trypsin inhibitor can be completely eliminated, and the content of the amino acid is also increased. The fermented soybean meal can be used for the preparation of the carnivorous fish feed additives instead of fish meal, in particular for the preparation of turbot feed. The fermented soybean meal containing the high content of protein can effectively replace 45% of fish meal protein of full fish meal feed when being used for the preparation of the turbot diet.

Description

(1) Technical field [0001] The invention relates to a fish feed additive, in particular to a preparation method of fermented soybean meal, a bacterial strain used for preparing fermented soybean meal and an application for preparing carnivorous fish feed additive. (2) Background technology [0002] The nutritional requirement of farmed fish for protein is about 35%-55% of the dry weight of their feed, which is 150%-300% higher than that of birds and mammals, and fish meal is the most important source of protein in the aquatic feed industry. Carnivorous fish has tender, delicious meat and high nutritional value, but there is a large demand for fishmeal. In recent years, with the rapid development of intensive farming, the demand for fishmeal has risen sharply, and the price of fishmeal has continued to rise, which has seriously hindered the development of aquaculture industry. Therefore, the development of new protein sources and the reduction of feed costs have become an ur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K10/18C12N1/14C12R1/665
CPCY02A40/818
Inventor 何艮张蓓莉皮雄娥王欣费迪波周慧慧刘伟麦康森
Owner OCEAN UNIV OF CHINA
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