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R.vinctus and application thereof in promoting aquilaria plants to produce agilawood

A technology of hard hole bacteria and hole bacteria, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of short distance, limited penetration ability, inability to be widely used, etc., and achieve strong environmental tolerance Strength, the effect of promoting aroma

Active Publication Date: 2015-08-19
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In this subject, through the research on the microbes of Akiras chinensis, it is found that fungi play an important role in the formation of agarwood, but the existing reported fungal strains and fungal aroma-promoting methods have a short distance of forming aroma and limited penetrating ability, so that they cannot be widely used. Therefore, it is of great significance to find strains that can improve the quality of agarwood and apply them to production

Method used

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  • R.vinctus and application thereof in promoting aquilaria plants to produce agilawood
  • R.vinctus and application thereof in promoting aquilaria plants to produce agilawood
  • R.vinctus and application thereof in promoting aquilaria plants to produce agilawood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Isolation, screening and identification of R.vinctus ASAF02 strain

[0042] The invention utilizes the tissue separation method to isolate the bacterial strain. The specific method is as follows: take a natural agarwood sample in Tongtieling, Wanning City, Hainan Province, rinse it under the tap for 30min, then disinfect it with 70% alcohol for 3-5min, rinse it with sterile water for 3 times, then rinse it with 10% ( mass percent concentration) of sodium hypochlorite for 5 minutes, then rinsed with sterile water for 3 times, then cut into 2mm-3mm segments and put them in the medium containing streptomycin, and the last sterile water was used as a control. After 3d, check and pick strains every day and purify until 2 weeks.

[0043] Cultivate the isolated strains in PDA liquid medium for 3-7 days, inoculate fungal hyphae, or use the whole body aroma technology to input fermentation broth and sterilized filtrate, and the extract containing active ingredients in...

Embodiment 2

[0046] Example 2 Detection of growth conditions of R. vinctus ASAF02 strain.

[0047] The hyphae of R.vinctus ASAF02 were inoculated in the center of the petri dish containing solid PDA medium, and were set at 10°C, 15°C, 20°C, 25°C, 28°C, 30°C, and 35°C. It was cultured in an incubator, and it was found that R.vinctus ASAF02 could grow at 15°C to 35°C, but 30°C was the most suitable for growth. Regulate the pH value of PDA medium with 0.1mol / L HCL and 0.1mol / L NaOH, make the liquid PDA medium that pH value is respectively 3,4,5,6,7,8,9,10,11, will The mycelium was inoculated in liquid PDA medium with different pH values, and cultured on a shaker at 30°C and 200 rpm. It was found that the strains could grow from pH3 to pH11, and the optimum pH value was 7.

Embodiment 3

[0048] Example 3 Verification of the effect of R.vinctus ASAF02 on promoting the production of agarwood

[0049] Cultivate the R.vinctus ASAF02 strain for 3-7 days to obtain strain mycelia, fermentation broth, and sterilized filtrate. According to the size of the tree, inoculate in different doses, drill holes, or use the whole body incense technology to inoculate the bacteria containing bacteria The fermented liquid, the filtrate of the fermented liquid to remove the bacterium is inoculated with white wood to promote the aroma, and the specific inoculation method is as follows:

[0050] 1. Acquisition of inoculation material: the mycelium obtained by culturing R.vinctus ASAF02 strain in liquid medium is the inoculation mycelium 1, which can be made into powder or ointment. The hyphae were inoculated into the PDA liquid medium, and shake cultured at 30° C. and 200 rpm for 3-7 days to obtain a culture solution, namely the bacterial solution 2. At the same time, after cultivati...

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PUM

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Abstract

The invention provides R.vinctus and an application thereof in promoting aquilaria plants to produce agilawood. The R.vinctus provided by the invention belongs to rigidoporus, meripilaceae, polyporales, agaricomycetes, basidiomycota, fungi. The R.vinctus provided by the invention is obtained by separating from aquilaria sinensis and is used for promoting the aquilaria with aquilaria sinensis as a representative to produce the agilawood, and a formed agilawood layer is relatively thick and has large penetration surface. The R.vinctus provided by the invention can improve the quality of the agilawood, and has great significance to production of the agilawood.

Description

technical field [0001] The invention relates to the field of microorganisms and their application, in particular to a Rigidoporus vinctus and its application for promoting the production of agarwood by agarwood plants. Background technique [0002] Agarwood is a kind of resin-containing wood of Aquilaria plants in the family Thymelaeaceae. The formation of agarwood is very special. Healthy white woods cannot produce agarwood substances, and only after external damage such as physical damage, chemical damage, and fungal infection It induces fragrance. High-quality agarwood takes years or even decades to develop. Due to the particularity of the formation of natural agarwood and the long cycle of agarwood formation, the technology of agarwood formation has become a research hotspot. [0003] In this subject, through the research on the microbes of Akiras chinensis, it is found that fungi play an important role in the formation of agarwood, but the existing reported fungal str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00A01G7/06C12R1/645
Inventor 陈旭玉魏建和甘炳春刘洋洋杨云苏智张争
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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