Method for improving silkworm nuclear polyhedrosis resistance through hybridization and backcross

Abstract

The invention discloses a method for improving silkworm nuclear polyhedrosis resistance through hybridization and backcross. Congestion circulation formed by a nuclear polyhedrosis silkworm resistance strain and three modes of hybridization, backcross and selfing of conventional good varieties, the genes of current good silkworm varieties are gradually introduced into the silkworm germplasm resistant to the nuclear polyhedrosis, meanwhile, the selection means of using a moth area half dividing method for adding BmNPV virus liquid is adopted, under the premise that the BmNPV resistant major gene is kept not lost, the economic character of BmNPV is improved, and the requirement for current production variety use is achieved. Then, the improved nuclear polyhedrosis silkworm resistance strain is in hybridization with the corresponding current good variety, and a generation of hybrid is produced. The generation of hybrid produced through the method has the high resistance to the nuclear polyhedrosis and meanwhile has the good economic character, and the generation of hybrid is widely applied and popularized in rural areas of national silkworm areas and is well received by silkworm raisers.

Description

technical field [0001] The invention relates to a hybridization and backcrossing method for improving silkworm resistance to blood type pus. Background technique [0002] Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) is the main virus that harms silkworms, and the blood-type pus disease caused by it ranks first in the virus diseases of silkworms. According to the survey of several major silkworm regions such as Jiangsu, Sichuan, Zhejiang, Guangxi, etc., the loss of pustular disease to sericulture industry accounts for more than 50% of the total loss of silkworm disease, and in some places it is as high as 80%, and there is even a severe situation of no harvest. Seriously dampened the enthusiasm of silkworm farmers and hindered the development of sericulture. In response to this phenomenon, sericulture scientists from China, Japan, France and other countries studied BmNPV from different angles, and carried out research on BmNPV-resistant silkworm breeding, and made some pro...

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Problems solved by technology

According to the survey of several major silkworm regions such as Jiangsu, Sichuan, Zhejiang, Guangxi, etc., the loss of pustular disease to sericulture industry accounts for more than 50% of the total loss of silkworm disease, and in some places it is as high as 80%, and there is even a severe situation of no harvest. Seriously dampened the enthusiasm of sericulture farmers and hindered the development of sericulture

In response to this phenomenon, sericulture scientists from China, Japan, France and other countries studied BmNPV from different angles, and carried out research on BmNPV-resistant silkworm breeding, and made some progress, but the practical level of these disease-resistant varieties is not enough. Cannot play an effective role in production

When the "National Silkworm Germplasm Resources Preservation Center of the Chinese Academy of Agricultural Sciences" of Jiangsu University of Science and Technology screened silkworm germplasm resources for anti-virus, it was found that a silkworm germplasm had strong disease resistance to BmNPV, and genetic analysis showed that its disease resistance was influenced by Dominant main effect gene control, but the economic status is very poor, far inferior to the current silkworm varieties in my country, and has no direct use value in production, so the germplasm must be improved by breeding

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