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Method of controlling vegetable soil pests via multiple Chinese caterpillar fungi

A technology of Cordyceps fungus and vegetable land, applied in the direction of botany equipment and methods, applications, pesticides, etc., can solve the problems of short survival time, inability to be used to control vegetable underground pests, harsh living conditions, etc.

Active Publication Date: 2015-08-19
YUNNAN MORALGOODS AGRI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Underground pests of vegetables mainly harm the underground parts of vegetables, often causing damage to roots, dead seedlings, and even lack of seedlings. The survival time is short and the survival conditions are harsh

Method used

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  • Method of controlling vegetable soil pests via multiple Chinese caterpillar fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Vegetable name: eggplant; main underground pests: grub; vegetable garden area: 1 mu; month: April.

[0017] Methods as below:

[0018] (1) Inoculate multiple Cordyceps bacteria into the culture medium, the culture medium components are: mashed potato 100g / L, egg white 15g / L, glucose or sucrose 4g / L, potassium dihydrogen phosphate 2g / L, magnesium chloride 1g / L, ferric citrate 0.05g / L, agar 4g / L, sterilized and poured into the culture plate to cool, after inoculation, cultivated in sterile dark light, temperature 28℃, humidity 80% for 26 hours, after taking out Cultivate for 9 hours under the environment of light and temperature of 28°C and humidity of 80%±5, then transfer to the environment of dark light and temperature of 25°C and humidity of 80% for 18 hours, take it out and put it in the environment of light and temperature of 30°C and humidity of 40% Induce spore production;

[0019] (2), put the culture plate that tawny beetle and mycelium are cultiva...

Embodiment 2

[0033] Embodiment 2. Vegetable name: Chinese cabbage; Main underground pest: mole cricket; Vegetable garden area: 300 square meters; Month: November.

[0034] Methods as below:

[0035] (1) Inoculate multiple Cordyceps fungi into the medium, the medium components are: mashed potato 99g / L, egg white 14L, glucose or sucrose 3.9L, potassium dihydrogen phosphate 1.9L, magnesium chloride 0.9g / L, Ferric citrate 0.04g / L, agar 4.1g / L, sterilized and poured into the culture plate to cool, after inoculation, cultured in sterile dark light, temperature 26 ℃, humidity 85% environment for 30 hours, took it out and placed it in the light Cultivate at a temperature of 30°C and a humidity of 85% for 12 hours, then transfer to a dark light environment and cultivate at a temperature of 26°C and a humidity of 85% for 12 hours. After taking it out, induce spore production under light and an environment of a temperature of 30°C and a humidity of 45% ;

[0036] (2), put the 5th instar larvae of N...

Embodiment 3

[0044]Example 3. Vegetable name: sweet potato; main underground pests: yellow ants; vegetable garden area: 2 mu; month: November.

[0045] Methods as below:

[0046] (1) Inoculate multiple Cordyceps fungi into the medium, the medium components are: mashed potato 99g / L, egg white 14L, glucose or sucrose 3.9L, potassium dihydrogen phosphate 1.9L, magnesium chloride 0.9g / L, Ferric citrate 0.04g / L, agar 4.1g / L, after sterilization, pour the culture tray to cool, after inoculation, culture in sterile dark light, temperature 25°C, humidity 75% environment for 30 hours, take it out and place it under light and Cultivate for 12 hours at a temperature of 30°C and a humidity of 75%, then transfer to a dark light environment and cultivate at a temperature of 25°C and a humidity of 75% for 12 hours, and then induce spore production under light and an environment of a temperature of 30°C and a humidity of 40%;

[0047] (2), the Asian migratory locust and the cultivation dish with mycelium...

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Abstract

The invention relates to a method of controlling vegetable soil pests via multiple Chinese caterpillar fungi and belongs to the technical field of vegetable cultivation. The method includes: cultivating multiple Chinese caterpillar fungi, infecting artificially bred insects with strains, drying or powdering the insects died of infection, during ridge rectifying before vegetable planting, burying the insects or the powder into soil, burying the insects or the powder one week after seedling planting or seedling emergence, and spreading the insects or the powder to the surface of the soil on a second week after vegetable seedling planting or emergence. The method has the advantages that the use of the single strains helps reduce competitive suppression effect of multiple strains during cultivating and greatly shorten the cultivation time of the strains and spores; the strain sores infected by the insects have a higher ability to survive in the natural environment, the storage requirements are lower, and the use of burying in application helps the multiple Chinese caterpillar fungi take effect longer in the soil and evidently enhance the effect of controlling soil superficial pests.

Description

technical field [0001] The invention belongs to the technical field of vegetable cultivation, and in particular relates to a method for preventing and controlling underground pests of vegetables. technical background [0002] One of the important ways to realize vegetable green cultivation or organic cultivation is to use biology to control pests. Cordyceps fungus is a type of fungus that lives in the body of insects. It infects insects through spore transmission, contact infection, etc., and uses the nutrients in the insect's body to mature quickly, causing the insect to die. The Chinese patent document with the application number 200410034974.8 has announced "Cordyceps fungus kills crop pest method", uses the extermination liquid prepared by Cordyceps sinensis, Cordyceps militaris, and Cordyceps gunis to spray on the leaf surface to kill pests on the ground. Underground pests of vegetables mainly harm the underground parts of vegetables, often causing damage to roots, dea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01N63/02A01P7/04
CPCA01G13/00A01N63/10
Inventor 田柏青
Owner YUNNAN MORALGOODS AGRI & TECH
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