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Application of hog cholera virus detection primer pair and detection kit

A detection kit, swine fever virus technology, applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of multiple reagents, multiple noises, affecting the accuracy or reliability of test results, etc., to avoid The effect of misdiagnosis, strong specificity and high detection rate

Inactive Publication Date: 2015-08-12
张家口市动物疫病预防控制中心
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Problems solved by technology

Typically, gene fragments larger than 1000nt have some problems, for example, more reagents are used compared to shorter gene fragments, and time-consuming
In addition, more importantly, more noise is likely to be generated during amplification, which will affect the accuracy or reliability of the detection results.

Method used

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  • Application of hog cholera virus detection primer pair and detection kit
  • Application of hog cholera virus detection primer pair and detection kit
  • Application of hog cholera virus detection primer pair and detection kit

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Embodiment Construction

[0027] Unless otherwise stated in the present invention, the nouns, terms, and phrases used in this application have the usual meanings in this field. In addition, unless otherwise specified in the present invention, the reagents, instruments, etc. used in this application are conventional reagents or instruments in the art, and they are freely available in the market.

[0028] The "swine fever virus" described in the present application is an ssRNA virus belonging to the Pestivirus genus of the family Flaviviridae, and its RNA is a single positive strand. It includes multiple virus species and subspecies, eg various serotype subspecies, different genotypes. In addition, it also includes various virus strains with strong, medium and weak virulence, for example: Shimen2004 (accession number: AY775178) and HCLV (accession number: AF091507) belonging to the attenuated strain of classical swine fever virus 1.1; HEBZ (accession number: GU592790), HEB5 (accession number: JQ268754) ...

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Abstract

A hog cholera virus nested RT-PCR detection kit is provided. A hog cholera virus PCR monitoring method is established on the basis of CSFV hog cholera wild virus strain complete genome sequence HEBZ (GU592790) included in Gen Bank as a reference, wherein amplified target fragment is 546 nt. The method is excellent in specificity, sensitivity and repeatability.

Description

technical field [0001] The invention relates to the use of a primer pair and a kit for nested RT-PCR detection of swine fever virus. Background technique [0002] Hogcholera virus (Swine fever virus) is the pathogen of swine fever, which harms pigs and wild boars. Swine fever is an acute, febrile, highly contagious infectious disease characterized by high temperature and microvascular degeneration that can cause systemic hemorrhage, necrosis, and infarction. Swine fever is extremely harmful to pigs and will cause heavy losses in the pig industry. [0003] Regarding the detection of swine fever, in 2007, Zhu Xiaofu et al. designed and synthesized primer pairs with reference to the gene sequence of the CSFV Shimen strain published on GenBank, wherein the primers used for amplification were in the E2 gene, and established a PCR detection method for CSFV ( Zhu Xiaofu et al. 2007). However, this method is based on the large variability in the E2 gene, thus, the sensitivity and...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6848C12Q2549/119
Inventor 杨若松
Owner 张家口市动物疫病预防控制中心
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