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A kind of polypeptide and its application and method for covalently linking polypeptide to solid phase interface

A solid-phase interface, covalent connection technology, applied in chemical instruments and methods, biochemical equipment and methods, peptides, etc., can solve the problems of time-consuming, inefficient, hindering the application of peptides, etc., and achieve high effective density and simple method. Effect

Inactive Publication Date: 2017-12-19
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the low immobilization density of peptides is also a defect that cannot be ignored in these methods.
Finally, most of these methods are time-consuming, laborious and inefficient, which greatly hinders the application of peptides in related fields.

Method used

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  • A kind of polypeptide and its application and method for covalently linking polypeptide to solid phase interface
  • A kind of polypeptide and its application and method for covalently linking polypeptide to solid phase interface
  • A kind of polypeptide and its application and method for covalently linking polypeptide to solid phase interface

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The polypeptide is shown by amino acid in SEQ ID No.1.

[0021] The sequence listing SEQ ID No.1 is:

[0022] AEGSDPRMV

[0023] (a) Sequential features:

[0024] ●Length: 9

[0025] ●Type: amino acid sequence

[0026] ●Chain type: single chain

[0027] ●Topological structure: linear

[0028] (b) Molecule Type: Protein

[0029] (c) Assumption: No

[0030] (d) Antisense: No

[0031] (e) Original Source: Artificial Design

Embodiment 2

[0033] Using polypeptide solid-phase synthesis technology, synthesize SEQ ID No.1 on the surface of Wang resin (with microsphere structure), and finally remove the protecting group. However, by maintaining the covalent connection between the polypeptide and the resin, the resin loaded symmetrically with the polypeptide SEQ ID No. 1 is obtained. In view of the density of active groups on the surface of Wang resin and the efficiency of solid-phase synthesis of peptides, the peptide sequence SEQ ID No. 1 can be covalently linked with high density and symmetry on the surface of Wang resin.

Embodiment 3

[0035] The polypeptide sequence SEQ ID No.1 is carried out through the standard genetic recombination operation in the prior art, using Ff phage as an axisymmetric carrier, and with the help of the phage VIII type display system, the amino acid sequence in the sequence table SEQ ID No.1 is loaded symmetrically On the surface of the phage, the organism (such as figure 1 shown). In view of the structure of the Ff phage itself and the characteristics of the VIII display system, each major capsid protein pVIII will be covalently linked to a polypeptide sequence SEQ ID No.1, thereby achieving a high degree of polypeptide sequence SEQ ID No.1 Density, symmetrical loading.

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Abstract

The invention belongs to the field of immobilization of biomolecules, in particular to a polypeptide and its application and a method for covalently linking the polypeptide to a solid phase interface. The polypeptide sequence is shown by amino acid in SEQ ID No.1. The polypeptide is used as a target recognition element for qualitative / quantitative monitoring of cellulase endoglucanase I (EG I). The invention realizes the immobilization of the polypeptide through the symmetrical carrier, so that the immobilized polypeptide has the characteristics of uniform orientation, high density and the like.

Description

technical field [0001] The invention belongs to the field of immobilization of biomolecules, in particular to a polypeptide and its application and a method for covalently linking the polypeptide to a solid phase interface. Background technique [0002] With the completion of the Human Genome Project, proteins will gradually become the focus of research in the field of life sciences. In view of the interaction between proteins and other biomolecules, such as proteins, sugars, lipids, etc., most of them are mediated by polypeptides. Therefore, peptides play a pivotal role in these interactions. In addition, the unique advantages of immobilization technology make peptide immobilization widely used in fields such as affinity purification, biosensing, biochips, and new materials. [0003] With the development of technology, more and more methods have been developed for the covalent attachment of peptides to solid phase interfaces. However, these methods either require modific...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K17/02C12Q1/34
Inventor 刘爱骅祁环
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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