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Culture method of holboellia fargesii reaub germchits

A cultivation method and seedling technology, applied in the field of plant cell engineering, can solve the problems of unreported and public application, and achieve the effects of short cultivation period, robust seedlings and high proliferation rate

Inactive Publication Date: 2015-07-22
JIUJIANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The cultivation and rapid propagation of Maymelon vine seedlings by means of plant tissue culture is a better method for obtaining Maymelon vine seedlings on a large scale, and this method has not been reported or publicly applied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method of using stem segments as explants and using plant tissue culture methods to comprehensively treat Maymelon vines for rapid propagation to obtain a large number of seedlings. The specific steps are as follows:

[0023] (1) Pretreatment: The pretreatment method is to soak the stem segment in an aqueous solution added with 4 g / L PVP+1.5 g / L Vc for 4 hours immediately after isolation, and then store it at 4°C for 9 days to keep it moist and refrigerated ;

[0024] (2) Obtaining sterile explants: the pretreated stem segments were sterilized with 75% alcohol for 30 seconds, then sterilized with mercury chloride for 10 minutes, and then rinsed with sterile water for later use;

[0025] (3) Callus induction culture: inoculate the obtained aseptic stem explants on liquid shallow callus induction medium for callus induction culture; the treatment method is: inoculate the obtained stem explants Cut to a length of 1 cm and inoculate on liquid superficial callus induction...

Embodiment 2

[0029] A kind of method that uses petiole as explant and utilizes plant tissue culture method to comprehensively process and carry out the rapid propagation of Maymelon and vine culture to obtain a large number of seedlings. The specific steps are as follows:

[0030] (1) Pretreatment: The pretreatment method is to soak the petiole in an aqueous solution added with 3 g / L PVP+1g / L Vc for 4 hours immediately after the petiole is isolated, and then put it in refrigerated storage at 4°C for 7 days;

[0031] (2) Obtaining sterile explants: pretreated petioles were sterilized with 75% alcohol for 30 seconds, sterilized with mercury chloride for 5 minutes, and then rinsed with sterile water for later use;

[0032] (3) Callus induction culture: inoculate the obtained sterile petiole explants on liquid shallow callus induction medium for callus induction culture; the processing method is: the obtained petiole sterile explants Cut to a length of 1 cm and inoculate them on liquid superfici...

Embodiment 3

[0036] A method of using leaves as explants and using plant tissue culture methods to comprehensively treat Maymelon and vines for rapid propagation to obtain a large number of seedlings. The specific steps are as follows:

[0037] (1) Pretreatment: The pretreatment method is to soak the leaves in an aqueous solution added with 3 g / L PVP+1 g / L Vc for 3 hours immediately after they are isolated, and then store them in refrigerated water at 4°C for 5 days.

[0038] (2) Obtaining sterile explants: the pretreated leaves were sterilized with 75% alcohol for 30 seconds, sterilized with mercury for 5 minutes, and then rinsed with sterile water for later use.

[0039] (3) Callus induction culture: inoculate the obtained sterile leaf explants on the liquid superficial callus induction culture medium for callus induction culture; the treatment method is: aseptically explant the obtained leaves Body cut to size 1cm 2 After inoculation on the liquid superficial callus induction medium, t...

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Abstract

The invention belongs to the technical field of plant cell engineering. A culture method of holboellia fargesii reaub germchits sequentially comprises the following steps: pre-treating, obtaining sterile explants, inducing calluses, carrying out induction and multiplication on cluster buds, carrying out rooting culture and transplanting. With the adoption of the technical scheme for producing the holboellia fargesii reaub germchits, the culture method has the beneficial effects that (1) the multiplication rate is high, the biological yield is great and the culture period is short; (2) the quality of tissue culture seedlings is good, the germchits are strong and the culture survival rate is high; the holboellia fargesii reaub germchits are cultured by using the method comprising the steps of pre-treating, obtaining the sterile explants, inducing the calluses, carrying out induction and multiplication on the cluster buds, carrying out rooting culture and transplanting; compared with other culture manners, the culture method has high multiplication efficiency, short culture time and convenience for transplanting; the germchits are strong, the culture survival rate is high and the cost is low; and therefore, the culture method is suitable for large-scale culture and production on the holboellia fargesii reaub germchits.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and in particular relates to a method for cultivating and rapidly propagating Maymelon vines to obtain a large number of seedlings through comprehensive treatment of plant tissue culture. Background technique [0002] Holboellia fargesii Reaub. is a plant of the genus Holboellia in the family Lardizabalaceae, which is produced in Yunnan, Guizhou, Sichuan, Hubei, Hunan, Shaanxi, Anhui, Guangxi, Guangdong and Fujian. Wildly distributed in hillside miscellaneous forests and valley forests at an altitude of 500-3000 meters. May melon vine is an evergreen woody vine with monoecious flowers, red, purple, dark purple, green, white or light yellow. Several flowers form corymb-style short racemes, which have high ornamental value and can be used as vertical greening materials. Cultivation; its flowering period is 4-5 months, and the fruiting period is 7-8 months. The fruit skin is purple,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 贾明良廖亮徐玲玲李同建方荷芳苏慧慧
Owner JIUJIANG UNIVERSITY
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