Cordycepin fermentation solid medium and preparation method and application thereof
A technology of solid culture medium and fermentation culture liquid, which is applied in the field of cordycepin fermentation solid medium and its preparation, can solve the problems of high production cost, difficult product purification, complicated synthesis process, etc. The effect of favoring accumulation
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Embodiment 1
[0029] Example 1: Preparation of solid medium for Cordyceps militaris fermentation.
[0030] 1) Glucose 42g / L, yeast extract 6g / L, peptone 10g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, Tween 80 2g / L, The solvent is water, and the pH value of the solution is adjusted to 5.8 after fully stirring and dissolving to obtain a fermentation culture solution.
[0031] 2) Mix and stir the fermentation medium in step 1) and the expanded perlite with a particle size of 6mm according to the volume-to-mass ratio of 40ml / g. After spray drying, the water content of the material is controlled at 4ml / g and put into a fermenter at 121°C. Steam sterilize for 20 minutes to obtain a solid culture medium for cordycepin fermentation.
Embodiment 2
[0032] Example 2: Preparation of Cordyceps militaris fermentation medium.
[0033] 1) Glucose 42g / L, yeast extract 6g / L, peptone 10g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, Tween 80 2g / L, The solvent is water, and the pH value of the solution is adjusted to 5.8 after fully stirring and dissolving to obtain a fermentation culture solution.
[0034] 2) Mix and stir the fermentation medium in step 1) and the expanded perlite with a particle size of 4mm according to the volume-to-mass ratio of 30ml / g. After spray drying, the water content of the material is controlled at 5ml / g and put into a fermenter at 121°C. Steam sterilize for 20 minutes to obtain a solid culture medium for cordycepin fermentation.
Embodiment 3
[0035] Example 3: Preparation of Cordyceps militaris fermentation medium.
[0036] 1) Glucose 42g / L, yeast extract 6g / L, peptone 10g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, Tween 80 2g / L, The solvent is water, and the pH value of the solution is adjusted to 5.8 after fully stirring and dissolving to obtain a fermentation culture solution.
[0037] 2) The fermentation culture liquid in step 1) is mixed with the expanded perlite with a particle size of 8mm according to the volume to mass ratio of 50ml / g. After spray drying, the water content of the material is controlled at 2.5ml / g and loaded into a fermenter. 121 ℃ steam sterilization for 20 minutes to obtain a solid medium for cordycepin fermentation.
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