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Method for inoculating fresh corn indoors by using pathogenic fusarium verticillioides

A technology of Fusarium verticillium and fresh corn, which is applied in the field of plant disease inoculation identification, can solve the problems of limited identification of corn materials, small amount of injected bacteria, and large environmental impact, so as to solve the problems of early identification, small environmental impact, The effect of a small amount of inoculum

Active Publication Date: 2015-07-15
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is also a method of combining soil burying inoculation with corn sowing and root burial inoculation at the adult plant stage. Generally speaking, this method inoculates a large amount of pathogenic bacteria, is labor-intensive, and time-consuming, and the identification of a large number of corn materials is limited.
Under field conditions, the infected corn kernels are buried in the root soil of the plant at 2 to 3 cm, which is greatly affected by the environment, and the effect and controllability are not strong
The stalk acupuncture method is to use a needle to pierce the base of the stalk to inject pathogenic bacteria into the tissue during the growth period of corn. It also has disadvantages such as labor-intensive, time-consuming, small amount of injected bacteria, and ineffective effects.

Method used

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  • Method for inoculating fresh corn indoors by using pathogenic fusarium verticillioides
  • Method for inoculating fresh corn indoors by using pathogenic fusarium verticillioides
  • Method for inoculating fresh corn indoors by using pathogenic fusarium verticillioides

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Embodiment 1

[0047] The invention provides a method for inoculating fresh corn indoors with pathogenic Fusarium verticillium, capable of identifying varieties and germplasm materials for resistance. The invention comprises the isolation and multiplication of the stem rot pathogenic Fusarium verticillium, the co-cultivation and inoculation of the pathogenic Fusarium verticillium and corn seeds and the identification of corn plant resistance.

[0048] The detailed process of the inoculation method of fresh sweet corn inbred line stem rot is as follows:

[0049] (1) Treatment of susceptible materials: Take diseased stalks from maize plants that have stalk rot under natural conditions in the field, cut them open, take the stem marrow tissue from the damaged parts, cut them into small pieces of 0.3-0.5cm, and use them under aseptic conditions. Soak in 0.1% mercuric chloride solution for about 1 min, rinse once with sterile water, take out the tissue block and disinfect it with 75% (v / v) alcohol...

Embodiment 2

[0066] The detailed process of the inoculation method for stem rot of fresh sweet corn varieties is as follows:

[0067] (1) Treatment of susceptible materials: Take diseased stalks from maize plants that have stalk rot under natural conditions in the field, cut them open, take the stem marrow tissue from the damaged parts, cut them into small pieces of 0.3-0.5cm, and use them under aseptic conditions. Soak in 0.1% mercuric chloride solution for about 1 min, rinse once with sterile water, take out the tissue block and disinfect it with 75% (v / v) alcohol for 5 mins, rinse with sterile water for 3 times, take out the material, inoculate on solid PDA medium, 26 ~28℃ incubator dark culture to isolate pathogenic bacteria;

[0068] (2) Isolation of the pathogenic Fusarium verticillium strain: the above-mentioned susceptible tissue was cultured on the medium for 2-3 days, and a single mycelium was picked from a culture dish with mycelium and transferred to a new solid PDA medium. Cu...

Embodiment 3

[0084] The detailed process of the inoculation method of fresh waxy corn germplasm material stem rot is as follows:

[0085] (1) Treatment of susceptible materials: Take diseased stalks from maize plants that have stalk rot under natural conditions in the field, cut them open, take the stem marrow tissue from the damaged parts, cut them into small pieces of 0.3-0.5cm, and use them under aseptic conditions. Soak in 0.1% mercuric chloride solution for about 1 min, rinse once with sterile water, take out the tissue block and disinfect it with 75% (v / v) alcohol for 5 mins, rinse with sterile water for 3 times, take out the material, inoculate on solid PDA medium, 26 ~28℃ incubator dark culture to isolate pathogenic bacteria;

[0086] (2) Isolation of the pathogenic Fusarium verticillium strain: the above-mentioned susceptible tissue was cultured on the medium for 2-3 days, and a single mycelium was picked from a culture dish with mycelium and transferred to a new solid PDA medium....

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Abstract

The invention discloses a method for inoculating fresh corn indoors by using pathogenic fusarium verticillioides. The method disclosed by the invention comprises the steps of separating to obtain bacterial stains of the pathogenic fusarium verticillioides; carrying out expanding propagation of the fusarium verticillioides; preparing a corn variety to be inoculated; carrying out co-culture inoculation on the fusarium verticillioides and corn seeds; seeding; carrying out evaluation of disease resistance of a corn plant. Without depending on natural conditions of land for growing field crops, the method disclosed by the invention is few in inoculated bacteria, little in environmental influence, better in effect and higher in controllability, can be combined into field inoculation tests, and verifies resistance of the variety on corn stalk rot in an auxiliary mode. The method disclosed by the invention provides an effective corn inoculation technology for fusarium verticillioides, and can solve the early evaluation problem of corn for stalk rot resistance.

Description

technical field [0001] The invention relates to plant disease inoculation identification technology, in particular to a method for indoor inoculation of fresh corn in the early stage of stem rot pathogenic fungus Fusarium verticillium. Background technique [0002] Corn is one of the most important food crops in the world, yet it can be infected by many soil-borne pathogens, resulting in yield loss and quality degradation. There are two common types of corn stalk rot, bacterial stalk rot and fungal stalk rot. Bacterial stalk rot is caused by bacterial infection of mid-developing maize plants causing stalk rot. Fungal stalk rot is an important soil-borne fungal disease caused by Fusarium or Pythium fungi that damage the base of corn roots and stems. The pathogen can infect the root system throughout the growth period and cause root rot. The primary root and adventitious root rot become shorter, the pith is hollow, the fibrous roots are few and black, and the entire root sys...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00A01G7/06C12N1/14C12R1/77
Inventor 李余良韩福光刘建华李春艳夏磊
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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