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A kind of production method of adenosine triphosphate (ATP)

A technology of adenosine triphosphate and production method, which is applied in the field of two-step fermentation of adenosine triphosphate fermentation and synthesis, can solve the problems of consumption, low recovery rate of adenosine triphosphate, equipment utilization rate, and many impurities, and achieve the goals of improving economic benefits, reducing pollution discharge, and simplifying the production process Effect

Inactive Publication Date: 2018-05-08
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the ion exchange adsorption process, ions and ATP compete for adsorption on the ion exchange resin, resulting in only about 5-13 grams of ATP adsorbed by the resin per 100 grams of resin (201×7). to 18-22g / 100g resin (201×7), but it needs to consume 4-5 times the volume of absolute ethanol of ATP fermentation broth
ATP recovery rate and equipment utilization rate are low, and there are many impurities in the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Add 400L of deionized water in the reaction pot, start the mixer, heat the jacket with hot water to make the water temperature in the reaction pot reach 40°C, add 280 kg of beer yeast sludge that has been frozen to dissolve it, and then add Add 100 kg of 40% glucose solution, 125 kg of 20% dipotassium hydrogen phosphate solution, and 100 kg of 20% potassium dihydrogen phosphate solution, adjust the pH value to 7.0, and ferment for 2.5 hours at a temperature of 40-42°C to extract invertase. Obtain fermented enzyme liquid.

[0043] The fermented enzyme extract liquid is first filtered through a plate frame, and the filter cloth is a polyester filter cloth with a precision of about 300 mesh. Then, it is filtered through a microfiltration membrane with a precision of 1 μm to obtain an enzyme-clearing solution. The form of the microfiltration membrane is flat plate, the membrane material is PP, the operating pressure is less than 0.5MPa, and the temperature is 30...

Embodiment 2

[0057] Example 2: Add 500L of deionized water in the reaction pot, start the mixer, heat the jacket with hot water to make the water temperature in the reaction pot reach 40°C, add 320 kg of beer yeast sludge that has been frozen to dissolve, and then add Add 120 kg of 40% glucose solution, 150 kg of 20% dipotassium hydrogen phosphate solution, and 100 kg of 20% potassium dihydrogen phosphate solution, adjust the pH value to 6.5, and ferment for 2.5 hours at a temperature of 38-40°C to extract invertase. Obtain fermented enzyme liquid.

[0058] The fermented enzyme extract liquid is first filtered through a plate frame, and the filter cloth is a polyester filter cloth with a precision of about 300 mesh. Then, it is filtered through a microfiltration membrane with a precision of 0.22 μm to obtain an enzyme-clearing solution. The form of the microfiltration membrane is flat plate, the membrane material is PP, the operating pressure is less than 0.5MPa, and the temperature is 30...

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Abstract

A production method of adenosine triphosphate (ATP) mainly includes following steps: (1) treatment of beer yeast; (2) fermentation to extract a conversion enzyme; (3) plate-frame pressure filtration; (4) micro-filtration; (5) fermentation to synthesize the ATP; (6) nano-filtering desalination; (7) resin adsorption; (8) resin washing elution; (9) ultra-filtration to remove protein and heat source; (8) precipitation with ethanol; and (9) a drying operation. In the invention, due to a high-efficient energy-saving membrane separation technology, after the step of fermentation to synthesize the ATP, the purity of the conversion enzyme can be increased through the micro-filtration, thereby increasing synthetic conversion rate of the ATP. During the separation and purification process of a fermentation synthetic liquid of the ATP, the nano-filtration is employed for removing inorganic salt nutrients added during the synthesis process so that the ATP adsorption capacity of ion exchange resin can be increased. By means of the ultra-filtration to remove protein and heat source, the purity of the ATP is increased. The production method can increase the production capacity, recycle rate and purity of the ATP, can reduce the consumption of chemical reagents, is simplified in processes, can reduce pollution emission and the like.

Description

technical field [0001] The invention relates to a method for producing adenosine triphosphate (ATP) by using adenosine (AR) as a raw material, and specifically relates to a two-step fermentation of adenosine triphosphate fermentation synthesis: the first step is to ferment and extract invertase, and the second step is to extract invertase under the catalysis of invertase Phosphorylates adenosine to adenosine triphosphate. Background technique [0002] Adenosine triphosphate (ATP) is a nucleotide drug composed of an adenine, a ribose sugar and a triphosphate unit, the molecular formula C 10 h 16 N 5 o 13 P 3 , the molecular weight is 507. It is the energy converter and storage in the living body, and plays an important role in the energy metabolism of the human body. ATP is also an important metabolite in organisms, as a metabolic intermediate and coenzyme, it participates in the metabolism of fat, protein, sugar and nucleic acid in organisms. In clinical application, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/32C07H19/20C07H1/06C12R1/865
CPCY02P20/10Y02P20/50
Inventor 黄宝能
Owner ZHEJIANG GONGSHANG UNIVERSITY
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