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Preparation of excellent single aseptic explants of Pinus massoniana and induction method of initial buds

A technology of explants and masson pine, which is applied in the field of plant propagation, can solve the problems of explant disinfection difficulties, low initial bud incidence, and poor activity of sprouts, and achieve high initial bud occurrence, good economic benefits and social benefits. Benefits, the effect of rapid elongation of sprouts

Active Publication Date: 2016-04-20
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical problems such as difficult explant disinfection, browning, low incidence of initial buds, poor activity of sprouts, etc. in the tissue culture of the existing excellent adult Pinus massoniana, and provides a preparation of excellent single-plant aseptic masson pine explants and the method of initial bud induction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for the preparation of aseptic explants of an excellent single plant of Masson pine and the initial bud induction method, using half-year-old masson pine tissue culture seedlings as the rootstock, and selecting the adult masson pine excellent plant GL38 from the excellent forest stand of Guangxi State-owned Paiyang Mountain Forest Farm Take tender shoots as scions. After the scion was cut from an adult good-quality masson pine mother tree, it was wrapped with a wet towel, put into an incubator equipped with ice packs, brought back to refrigerate at 4°C, and grafted by conventional methods within 24 hours. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouting strips reached 3.0-5.0 cm in length, the conventional method was used to spray 0.1% carbendazim at a volume concentration, and after 1-2 days, they were harvested in fine weather and vigorously harvested. The long sprout stri...

Embodiment 2

[0027] A method for the preparation of aseptic explants of an excellent single plant of Masson pine and the initial bud induction method, using half-year-old masson pine tissue culture seedlings as the rootstock, and selecting the adult masson pine excellent plant GL38 from the excellent forest stand of Guangxi State-owned Paiyang Mountain Forest Farm Take tender shoots as scions. After the scion was cut from an adult good-quality masson pine mother tree, it was wrapped with a wet towel, put into an incubator equipped with ice packs, brought back to refrigerate at 4°C, and grafted by conventional methods within 24 hours. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouting strips reached 3.0-5.0 cm in length, the conventional method was used to spray a 0.1% carbendazim solution with a volume concentration. After 1-2 days, the harvested plants were vigorous in fine weather. , and the long sprout...

Embodiment 3

[0032] A method for the preparation of aseptic explants of an excellent single plant of Masson pine and the initial bud induction method, using half-year-old tissue cultured seedlings of Masson pine as rootstock, and selecting the adult masson pine excellent plant GL105 from the excellent forest stand of Guangxi State-owned Paiyang Mountain Forest Farm. Take tender shoots as scions. After the scion was cut from an adult good-quality masson pine mother tree, it was wrapped with a wet towel, put into an incubator equipped with ice packs, brought back to refrigerate at 4°C, and grafted by conventional methods within 24 hours. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouting strips reached 3.0-5.0 cm long, the conventional method was used to spray 0.1% thiophanate-methyl solution, and 1-2 days later, the vigor was harvested in fine weather. Exuberant, long sprouting strips of 3.0-5.0 cm are use...

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Abstract

The invention discloses a method for preparation of a pinus massoniana lamb superior individual sterile explant and induction of an initial bud. The method comprises the following steps: grafting by selecting a sprout twig with a newly grown tender top in the current year of an adult superior individual as a scion and taking a pinus massoniana lamb tissue culture seedling as a stock, performing disinfection treatment on the sprout twig by adopting a series of disinfectants to match for disinfection, and putting the explant scion in an optimized induction culture medium to perform initial bud induction to obtain the sterile explant and the pinus massoniana lamb initial bud with rapid growth and vigorous vitality. By adopting the method disclosed by the invention, the technical problems of difficulty in disinfection of the explant, browning, low incidence of the initial bud, poor activity of a bud shoot and the like in tissue culture of the pinus massoniana lamb adult superior individual are solved, the incidence of the initial bud and the activity of the bud shoot are improved, the robust explant which is sterile above 90% can be obtained, and better economic benefits and social benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to tissue culture and seedling raising technology, in particular to a method for preparing excellent single aseptic explants of Pinus massoniana and inducing initial buds. Background technique [0002] Masson pine (Pinusmassoniana Lamb.) belongs to Pinaceae (Pinaceae), Pinus subfamily (Pinoideae), Pinus L. (SubgenPinus) plant, and is a unique native afforestation resin dual-purpose tree species in subtropical regions of my country. As a tree species with high comprehensive utilization value and broad promotion prospects, masson pine can not only be used to produce three-board, papermaking and chemical fiber industrial manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. In recent years, with the intensification of contradictions between people's growing living needs and the shortage of timber resources and the gradual depletion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 姚瑞玲王胤苏烜雷军芳
Owner GUANGXI FORESTRY RES INST
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